Role of Contractile Microfilaments in Macrophage Movement and Endocytosis

Allison, A. C.; Davies, P.L.; Petris, S. de

doi:10.1038/newbio232153a0

Cited by 322 publications

(103 citation statements)

References 7 publications

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“…The change in shape effected by this agent in some cell types, giving rise to multiprocessed cells of a remarkable dendriform or arborized appearance has been depicted by other observers (6,61,78) . The importance of the strong, generalized contraction of ectoplasm as an almost immediate consequence of the action of cytochalasin, seems not to have been appreciated hitherto .…”

Section: Contractionmentioning

confidence: 99%

“…These effects of cytochalasin included, inter alia, flattening and "relaxation" of cells, (whence the name cytochalasin), paralysis of locomotion and of membrane movements (17,35,86,103), expulsion of the cell nucleus (17,74), prevention of cytokinesis (17,35,81), suppression of pinocytosis (6,95) and of phagocytosis (6,26,57,114), interference with platelet function and clot retraction (83), prevention of morphogenetic movements in epithelia (8,56,85,110), of axonal growth (111), cell sorting (89), suppression of cytoplasmic streaming (11,106), inhibition of secretion (14,63,80,105), blocking of the interaction of myosin and actin in vitro (88), the assembly of myofibrils, and contraction of cardiac muscle cells (58) .…”

Section: Introductionmentioning

confidence: 99%

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Action of Cytochalasin D on Cells of Established Lines

Miranda

Godman

Deitch

et al. 1974

The Journal of Cell Biology

216

119

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HeLa, Vero, L, HEp2, and MDBK cells respond immediately to 0.2–0.5 µg/ml cytochalasin D (CD) with sustained contraction (contracture), loss of microvilli, expression of endoplasmic contents (zeiosis), nuclear protrusion, and extension of cytoplasmic processes. The development of these changes is depicted, and the dose-response patterns in these cell lines are described. MDBK is generally most resistant and HeLa most sensitive to these effects of CD. Cells in G1 are most sensitive to CD; responsiveness decreases progressively during early S and is least in mid S through G2. CD inhibits transport of [14C]deoxyglucose in HeLa by about 45% but has no significant effect on hexose uptake in Vero and MDBK; sugar transport is thus apparently unrelated to any morphologic effect of CD. Although spreading and attachment are impeded, CD does not decrease and may even enhance the adhesiveness of established monolayers. Contraction appears to be a primary early effect of CD, upon which other visible changes follow. It is prevented by some inhibitors of energy metabolism (deoxyglucose and dinitrophenol) and does not occur in glycerinated models without ATP. The possible bases of the contractile response to CD are discussed. Although direct or indirect action of CD on some microfilaments may occur, a generalized structural disruption of contractile filaments by CD is considered unlikely.

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Section: Contractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Action of Cytochalasin D on Cells of Established Lines

Miranda

Godman

Deitch

et al. 1974

The Journal of Cell Biology

216

119

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“…Samples of the medium were then treated with NP40 and specifically precipitated. associated events such as pinocytosis and "cap" formation (cytochalasin) (16)(17)(18)(19). Therefore, samples of radiolabeled cells were incubated for 3 hr with concentrations of such agents that did not affect cell viability (10-4-10 -5 M).…”

Section: E ~Rect Of Temperature and Pharmacologic A Gents On Release mentioning

confidence: 99%

Cell Surface Immunoglobulin

Vitetta

Uhr

1972

The Journal of Experimental Medicine

108

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Turnover and release of cell surface Ig and secretion of total intracellular Ig has been studied in small lymphocytes from normal mouse spleen. The major findings to emerge are: (a) small lymphocytes secrete 8S IgM and IgG. A small portion of the 8S IgM, but virtually none of the IgG appears to have a cell surface phase. (b) Cell surface IgM is actively turned over with a half-life of 6–8 hr, and turnover can be accounted for by release into the incubation medium. Release is temperature dependent. (c) Released cell surface Ig is noncovalently bound to a fragment of plasma membrane. (d) H-2 antigens are not released during short-term incubation. Based on the above findings, we propose a model for the transport and release of both cell surface and conventionally secreted Ig.

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“…These movements seem to be related to a membraneassociated microfilament system (14,27,28,36,38) that has frequently been shown to contain actin (2,22,24,29,30). Many morphological studies showing disruption of thin filament arrays (3,6,7,18,31,32,39,40) and a few studies with purified actin (16,21,33,34) or myosin (footnote 2 and reference 26) suggest that cytochalasins can interact directly with proteins of the microfilament system.…”

mentioning

confidence: 99%

Cytochalasin B inhibits actin-related gelation of HeLa cell extracts.

Weihing¹

1976

The Journal of Cell Biology

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When the 100,000 g supernatant fraction (extract) of HeLa cells lysed in a buffer containing sucrose, ATP, DTE, EGTA, imidazole, and Triton X-100 is incubated at 25 degrees C, it gels, and actin and a HMWP are progressively enriched in the extract and in gel isolated from extract. CB (greater than or equal to 0.25 muM) inhibits gelation and specifically lowers the concentrations of actin and the HMWP in the fraction which sediments at 100,000 g after incubation. These results indicate that actin and HMWP are partly disaggregated by cytochalasin treatment, and thus that their aggregation is related gelation. Inasmuch as previous results showed that actin is present and HMWP is enriched in the plasma membrane fraction of HeLa cells, the results also point to a possible relation between plasma membrane-associated gel and in vivo effects of CB.

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Role of Contractile Microfilaments in Macrophage Movement and Endocytosis

Cited by 322 publications

References 7 publications

Action of Cytochalasin D on Cells of Established Lines

Action of Cytochalasin D on Cells of Established Lines

Cell Surface Immunoglobulin

Cytochalasin B inhibits actin-related gelation of HeLa cell extracts.

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