Cell Surface Immunoglobulin

Vitetta, Ellen S.; Uhr, Jonathan W.

doi:10.1084/jem.136.4.676

Cited by 108 publications

(24 citation statements)

References 39 publications

(33 reference statements)

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“…The present findings extend earlier observations (10) and suggest that cell death is not responsible for these findings. Not only did release occur without evidence of cell death, but release slowed as cells began to die 6-24 h after cultivation.…”

Section: Ssupporting

confidence: 81%

“…In several experiments precipitates were reduced and alkylated (11), dialyzed (11) and electrophoresed on 5% acrylamide gels in sodium dodecyl sulfate (SDS), or electrophoresed without reduction and alkylation on agarose-2.5% acrylamide gels (10). #, ~, and L chains or 19S IgM, 8S IgM, and 7S IgG served as markers on companion gels.…”

Section: Methodsmentioning

confidence: 99%

“…Their findings are not incompatible with those reported here. We have suggested that H-2 alloantigens could be molecules in the walls of post-Golgi vesicles that shuttle to and from the plasma membrane transporting Ig (and other proteins) to the plasma membrane for exteriorization (10,17). This concept implies that H-2 antigens alternately become exteriorized and interiorized and that degradation (and synthesis) could occur at a slow rate.…”

Section: -1-2 Antigen and Io In Murine Splenocytesmentioning

confidence: 99%

“…The first point to be made is that Ig as well as many other cell-surface proteins are released on a fragment of plasma membrane (10). We suggest as a working hypothesis that Ig and these other cell-surface proteins represent peripheral proteins in the fluid mosiac model of Singer and Nicholson (16), whereas H-2 alloantigens represent integral ones.…”

Section: Smentioning

confidence: 99%

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Synthesis, Intracellular Distribution, and Secretion of Immunoglobulin and H-2 Antigen in Murine Splenocytes

Wernet

Vitetta

Uhr

et al. 1973

The Journal of Experimental Medicine

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Despite considerable interest in the genetic control and biochemistry of histocompatibility antigens (1), virtually nothing is known of their biosynthesis, intracellular transport, insertion into the plasma membrane, and subsequent fate. It has been suggested that histocompatibility (H-2) 1 alloantigens are primarily confined to the plasma membrane (2, 3). 1-1-2 alloantigens have been described in subcellular fractions such as the microsomes (4-6) ; however, the extent of contamination of these fractions by plasma membrane (7) was not adequately assessed.We have previously studied the biosynthesis and intracellular fate of immunoglobulin (Ig) in plasma cells and lymphocytes using two approaches: (a) labeling of Ig with precursors, subcellular fractionation, disruption of fractions in detergent, and isolation of radioactive Ig by means of specific immunoprecipitation (8); (b) enzymatic radioiodination of cell-surface Ig followed by cell lysis and immunoprecipitation of surface molecules of high specific activity (9) and determination of release of cell-surface Ig from cultured radiolabeled cells (10).In the present studies, these approaches have been applied to a study of H-2 alloantigen and Ig in splenocytes from normal A / J mice. The results suggest that both macromolecules remain membrane bound from synthesis until exteriorization.

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Section: Ssupporting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Section: -1-2 Antigen and Io In Murine Splenocytesmentioning

confidence: 99%

Section: Smentioning

confidence: 99%

See 2 more Smart Citations

Synthesis, Intracellular Distribution, and Secretion of Immunoglobulin and H-2 Antigen in Murine Splenocytes

Wernet

Vitetta

Uhr

et al. 1973

The Journal of Experimental Medicine

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“…Our earlier studies also reported that IgM was the dominant Ig on the surface of marine splenocytes even when substantial amounts of IgG were being synthesized (4). Three experiments were performed in which thoracic duct cells were enzymatically radioiodinated and the resultant cell lysates acid-and immuno-precipitated.…”

Section: Resultsmentioning

confidence: 99%

Cell Surface Immunoglobulin

Uhr

Vitetta

1974

The Journal of Experimental Medicine

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F o r reasons not relevant to the present studies, thoracic d u c t l y m p h o c y t e s from y o u n g B A L B / c mice were observed to synthesize an H chain with a different mobility from/~-chain. This finding was unexpected because the H chains synthesized b y and on the surface of splenocytes from similar animals are almost exclusively/z-chain (1). T o investigate this finding further, thoracic duct l y m p h o c y t e s and splenocytes of y o u n g B a l b / c mice were labeled with [3H]tyrosine or were surface radioiodinated with 125I. T h e classes of Ig synthesized and secreted b y these cells and a t t a c h e d to their cell surfaces were determined. Materials and MethodsPreparation of Cell Suspension.--Male Balb/c mice of 21~-3~ mo of age were purchased from Jackson Laboratories, Bar Harbor, Maine. Cell suspensions were prepared from perfused spleens and thoracic duct lymphocytes were obtained (2) from the first 18-24 h of drainage. 6-wk old germ-free mice (C3H) were obtained from Sprague Dawley, Madison Wis.Radiolabding of Cdls.--Cells were iodinated as previously described (1) at a conc'entration of 108 cells/ml with 2 mCi of 126I, 50/zg of lactoperoxldase (Sigma Chemical Co., Inc., St. Louis, Mo.) and 25 #1 of 0.03% hydrogen peroxide. The reaction was terminated after 5-10 rain by the addition of 25 volumes of cold phosphate-buffered saline pH 7.3 (PBS). Cells were centrifuged, washed once in PBS and once in Eagle's Minimal Essential Medium (MEM) (Grand Island Biological Corp., New York).For labe]ing with [3H]tyrosine, cells were washed in MEM containing 10% fetal calf serum and 2% antibiofic-anfimycotic mixture and lacking tyrosine. Cells were suspended at 5 X 10e/m]. 40-100/zCi/ml of L-tyrosine (3,5-3H) (New England Nuclear, Boston, Mass.) were added and the cultures were incubated at 37°C in a moist CO2 environment.

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Morphological and immunocytochemical studies of fibronectin‐coated, plasma membrane‐limited vesicles in the early chicken embryo

1988

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Vesticles with a mean outside diameter of 32.8 nm have been observed in the early chicken embryo after fixation with a mixture of glutaraldehyde and tannic acid. Densitometric tracing has revealed that the vesicles are limited by a unit membrane. The presence of complex carbohydrates is suggested by the increased electron density of the vesticles after addition of tannic acid to the fixative. Immunocytochemical staining with a monoclonal antibody directed against chicken cellular fibronectin demonstrated the presence of this glycoprotein along the surface of the vesicles. These results suggest a cellular origin of the vesicles, since their surface shares morphological and biochemical similarities with the cell surfaces of the embryonic tissue layers. Recycling of plasma-membrane vesicles may occur, as vesicles were found in the vicinity of coated vesicles. We postulate that extracellular materials of the cell surface, which may affect cell and tissue interactions, are shed in the environment together with plasma-membrane vesicles. The difficulties encountered in observing the vesicles stems from the facts that an adequate visualization method is necessary and that they are few in number. The latter reason suggests their transient nature. The vesicles probably rapidly disintegrate in the extracellular milieu or are recycled by the cell surface.

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Cell Surface Immunoglobulin

Cited by 108 publications

References 39 publications

Synthesis, Intracellular Distribution, and Secretion of Immunoglobulin and H-2 Antigen in Murine Splenocytes

Synthesis, Intracellular Distribution, and Secretion of Immunoglobulin and H-2 Antigen in Murine Splenocytes

Cell Surface Immunoglobulin

Morphological and immunocytochemical studies of fibronectin‐coated, plasma membrane‐limited vesicles in the early chicken embryo

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