Role of Caspases, Bax and Bcl-2 in Chrysin-Induced Apoptosis in the A549 Human Lung Adenocarcinoma Epithelial Cells

Samarghandian, Saeed; Nezhad, Mohsen Azimi; Mohammadi, Gholamreza

doi:10.2174/1871520614666140209144042

Cited by 75 publications

(46 citation statements)

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“…For the cells treated with chrysin, cells stained with DAB (indicating apoptotic changes) were significantly increased compared to the controls. This is consistent with previous studies, which have reported an apoptosis-induction effect of chrysin in prostate cancer, lung cancer, leukemia and cutaneous melanoma cells (4,5,9,12).…”

Section: Discussionsupporting

confidence: 94%

“…However, the activities of caspase-8 were not altered following treatment with chrysin. These observations are consistent with previous reports (9,12), that suggested that chrysin induced apoptosis in cutaneous melanoma cells and lung adenocarcinoma epithelial cells primarily via the intrinsic mitochondrial signaling pathway.…”

Section: A B Csupporting

confidence: 94%

“…Previous studies have revealed that chrysin induces apoptosis of malignant cells through the mitochondrial signaling pathway (9,12). However, Monasterio et al (4) reported that chrysin induced human leukemia U937 cell apoptosis in a manner that required the activation of caspase-8 instead of caspase-9, which is the extrinsic signaling pathway.…”

Section: A B Cmentioning

confidence: 98%

“…Chrysin was initially identified for its anti-oxidant effects and has been shown to possess cancer chemopreventive and anti-tumor effects (6)(7)(8). It inhibits proliferation and induces apoptosis in a variety of cancer cells in vitro, including cells from the prostate, skin, breast, lung, cervix, thyroid cancer and leukemia (5,(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). However, to the best of our knowledge, the effects of chrysin on uveal melanoma cells have not been previously studied.…”

Section: Introductionmentioning

confidence: 99%

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Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Xue

Chen

et al. 2016

Oncology Letters

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Abstract. Uveal melanoma is the most common intraocular malignant tumor in adults. Chrysin is a flavonoid present in honey, propolis, various plants and herbs. In the present study, the cytotoxic effects of chrysin were investigated on human uveal melanoma cell lines (M17 and SP6.5) and associated signaling pathways, and a comparison to the effects on normal ocular cells [scleral fibroblasts and retinal pigment epithelial (RPE) cells] was performed. The effects of chrysin on cell viability were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was determined by using terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling assay. Mitochondrial permeability was determined by JC-1 fluorescein analysis. Cytosol cytochrome c levels, and the activities of caspase-3, -8 and -9 were measured by enzyme-linked immunosorbent assay or colorimetric assay. Chrysin reduced the viability of cultured human melanoma cells in a dose-dependent manner (0, 10, 30 and 100 µM) with IC 50 at 28.3 and 35.8 µM in SP6.5 and M17 cell lines, respectively. Chrysin at 30-100 µM levels selectively reduced the viability of melanoma cells without affecting the viability of scleral fibroblasts and RPE cells. Chrysin increased mitochondrial permeability, the levels of cytosol cytochrome c, and caspase-9 and -3 activities, but not capase-8 activity in uveal melanoma cells. The results of the present study indicate that chrysin induces apoptosis of human uveal melanoma cells via the mitochondrial signaling pathway and suggest that chrysin may be a promising agent in the treatment of uveal melanoma.

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Section: Discussionsupporting

confidence: 94%

Section: A B Csupporting

confidence: 94%

Section: A B Cmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Xue

Chen

et al. 2016

Oncology Letters

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“…The effect of Bcl-2 depends on the ratio of its expression with Bax; this ratio determines whether cells undergo apoptosis or survival upon signal stimulation (35). Excessive Bax expression in cells promotes apoptosis, whereas excessive Bcl-2 expression promotes survival (36).…”

Section: Discussionmentioning

confidence: 99%

Antitumor activity of the Ailanthus altissima bark phytochemical ailanthone against breast cancer MCF‑7 cells

Wang

et al. 2018

Oncol Lett

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Abstract. Ailanthone is isolated from the bark of Ailanthus altissima (Mill.) Swingle (Simaroubaceae). The mechanism that underlies the activity of ailanthone on MCF-7 cells was investigated by MTT assay. Breast cancer MCF-7 cells were treated with 0.5, 1.0, 2.0, 4.0 and 8.0 µg/ml ailanthone for 24, 48 and 72 h. The inhibition of proliferation induced by treatment with ailanthone was assessed by MTT assay. Apoptosis and cell cycle distribution in MCF-7 cells with the same doses of ailanthone for 48 h were determined by flow cytometry. Expression of apoptosis-associated genes and proteins were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis, respectively. The results revealed that ailanthone inhibited MCF-7 cell proliferation. Flow cytometry assay demonstrated that ailanthone induced apoptosis and G 0 /G 1 cell cycle arrest in MCF-7 cells. Western blotting and RT-PCR assays demonstrated that upregulation of pro-apoptotic caspase-3 and Bcl-associated X, and downregulation of anti-apoptotic apoptosis regulator B-cell lymphoma-2 in MCF-7 cells may be associated with the induction of apoptosis and inhibition of proliferation. To the best of our knowledge, the present study is the first to investigate the antitumor activity of ailanthone from A. altissima on MCF-7 cells and to attempt to elucidate the underlying mechanism. The present study revealed the presence of ailanthone-mediated antitumor effects, indicating that ailanthone may be a novel phytomedicine with potential use in breast cancer therapy.

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Nutraceuticals for Prevention and Treatment of Cancer

Das

Nathani

Kumar

et al. 2022

Handbook of Nutraceuticals and Natural Products

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Role of Caspases, Bax and Bcl-2 in Chrysin-Induced Apoptosis in the A549 Human Lung Adenocarcinoma Epithelial Cells

Abstract: This study shows that chrysin could also be considered as a promising chemotherapeutic agent and anticancer activity in treatment of the lung cancer cells in future.

Cited by 75 publications

References 0 publications

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Antitumor activity of the Ailanthus altissima bark phytochemical ailanthone against breast cancer MCF‑7 cells

Nutraceuticals for Prevention and Treatment of Cancer

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