Role of carbohydrates in micropropagation of cork oak

Romano, Anabela; Noronha, Catarina; Martins-Loução, Maria Amélia

doi:10.1007/bf00037670

Cited by 71 publications

(43 citation statements)

References 26 publications

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“…Among the three carbon sources, sucrose proved to be better for shoot regeneration than fructose and glucose (data not shown). Similar results were obtained in micropropagation of Cork oak (Romano et al, 1995) and Kaempferi (Shirin et al, 2000). However, sucrose and glucose induced the highest frequency of organogenesis in Bixa orellana (De Paiva Neto et al, 2003).…”

supporting

confidence: 81%

An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

Baskaran

Jayabalan

2005

In Vitro Cell. Dev. Biol. - Plant

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SummaryAn efficient rapid and large-scale in vitro clonal propagation of the valuable medicinal herb Eclipta alba (Asteraceae) by enhanced axillary shoot proliferation in cotyledonary node segments was designed. The medium type, various carbon sources, plant growth regulators, and coconut water markedly influenced in vitro propagation of Eclipta alba. An in vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of benzyladenine (4.4 mM), kinetin (4.6 mM), 2-isopentenyladenine (4.9 mM), gibberellic acid (1.4 mM), 5% coconut water, and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length. Subculturing of cotyledonary node segments on a similar medium enabled continuous production of healthy shoots with similar frequency. Rooting was highest (94.3%) on full strength MS medium containing 9.8 mM indolebutyric acid. Micropropagated plants established in garden soil, farmyard soil, and sand (2:1:1) were uniform and identical to the donor plant with respect to growth characteristics as well as floral features. These plants grew normally without showing any morphological variation.

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confidence: 81%

An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

Baskaran

Jayabalan

2005

In Vitro Cell. Dev. Biol. - Plant

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“…Overall, sucrose at 3% (w/v) resulted in higher multiplication of shoots than glucose at all concentrations tested, however, differences were not significant. A number of studies advocate the use of 3% sucrose in culture media (Yaseen et al 2012) and similar results have been reported in Amaryllis belladonna (De-Bruyn 1992), Eclipta alba (Baskaran and Jayabalan 2005), and Quercus suber (Romano et al 1995).…”

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confidence: 52%

In vitro propagation of cherry birch (Betula lentaL.)

Rathwell¹,

Shukla²,

Jones³

et al. 2016

Can. J. Plant Sci.

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An efficient procedure for the micropropagation and conservation of cherry birch (Betula lenta L.), an endangered species in Canada, is reported. The model utilizes in vitro proliferation of fresh and dormant buds from both greenhouse and mature trees. Various factors were evaluated to optimize the protocol, including plant growth regulators, type and concentration of carbohydrates, and the composition of basal salts. 6-benzylaminopurine was the most effective cytokinin for shoot multiplication, with an optimal concentration of 5 μM. Sucrose was a more effective carbohydrate source than glucose, with the highest multiplication rate occurring at 3% sucrose (w/v). Shoot multiplication was similar on Murashige and Skoog (MS) and Driver and Kuniyuki Walnut (DKW) basal salts, but significantly lower on Woody Plant medium (WPM). Culture media with half-strength DKW basal salts and 20 μM indole-3-butyric acid was found to be the best for rooting (80%). Rooted plantlets were acclimatized to the greenhouse environment with a 37% survival rate. The micropropagation technology developed in the study offers excellent opportunities and tools for rapid replenishment of cherry birch trees in their natural environment, long-term conservation, and provides a platform for further research of this nationally endangered species.Key words: micropropagation, Betula lenta, cherry birch, conservation.Résumé : Les auteurs présentent une méthode efficace pour la micropropagation et la préservation du bouleau flexible (Betula lenta L.), une espèce menacée d'extinction au Canada. Le modèle fait appel à la multiplication in vitro de bourgeons frais et dormants prélevés sur des arbres cultivés en serre et matures. Plusieurs facteurs ont été évalués de manière à optimiser le protocole, notamment les hormones de croissance végétales, la nature et la concentration des hydrates de carbone, ainsi que la composition des sels de base. La 6-benzylaminopurine est la meilleure cytokinine pour multiplier les pousses et sa concentration optimale est de 5 μM. Le sucrose est plus efficace que le glucose comme source d'hydrates de carbone, le taux de multiplication le plus élevé ayant été enregistré à la concentration de 3 % (p/v). Les sels de base Murashige and Skoog (MS) et Driver and Kuniyuki Walnut (DKW) permettent une multiplication similaire des pousses, mais celle-ci est significativement plus faible quand on utilise le milieu de culture Woody Plant (WPM). Le milieu de culture contenant la moitié de la dose recommandée de DKW et 20 μM d'acide indole-3-butyrique est le meilleur pour l'enracinement (80 %). Trente-sept pour cent des plantules enracinées ont survécu en serre après acclimatation. La technique de la micropropagation élaborée dans le cadre de cette étude présente d'excellentes possibilités et offre des outils intéressants pour rétablir rapidement la population de bouleaux flexibles dans leur milieu naturel, pour la conservation à long terme et pour des recherches plus poussées sur cette espèce en voie de disparition au pays. [Tra...

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“…Copyright © 2010 The Japanese Society for Plant Cell and Molecular Biology crispa, A. cordata and A. rubra (Barghchi 1988;Welander et al 1989), Potentilla fruticosa and Ficus lyrata (Wainwright and Scrace 1989), and cork oak (Quercus suber) (Romano et al 1995). Root induction was observed in liquid modified WPM supplemented with 49.20 m M IBA after three weeks of culture ( Figure 1C, D).…”

mentioning

confidence: 97%

In vitro propagation of pecan [Carya illinoinensis (Wangenh) K. Koch]

Renukdas

Manoharan

Garner

2010

Plant Biotechnology

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The pecan [Carya illinoinensis (Wangenh.) K. Koch], a member of the family Juglandaceae, is an economically important nut crop. Propagation of pecan is done primarily by budding or grafting of improved cultivars onto seedling rootstocks. However, these methods suffer disadvantages such as considerable time, expense and poor transplanting survival of the plants. Propagation by cutting, though leads to uniform clonal rootstocks, is very difficult as the clones do not root readily. Even with the use of hormones, inconsistent results with only limited improvements have been reported (Brutsch et al. 1977;Smith et al. 1974;Wolstenholme and Allan 1975). In vitro propagation offers great potential for the pecan industry for large scale multiplication of selected clones and enables production of a high amount of constant quality plantlets within a very short time. Although somatic embryogenesis has been developed for pecan (Mathews and Wetzstein 1993;Merkle et al. 1987;Wetzstein et al. 1989Wetzstein et al. , 1990Yates and Reilly 1990), successful pecan micropropagation was not achieved because of poor regeneration and rooting. Wood (1982) and Knox and Smith (1981) have used nodal stem segment explants from seedlings to establish shoot proliferation, however, no consistent shoot proliferation, elongation or root development occurred and no plants were established in soil. Phillips and Ramirez (1983) and Corte-Olivares et al. (1990) used apical and axillary buds; shoot elongation was obtained, but rooting and establishment of plantlets were poor. Hansen and Lazarte (1984) used nodal cuttings as explants; shoots were rooted with 93% success and 63% plants were established in soil while Corte-Olivares et al. (1990) obtained 40% rooting of shoots. In this article, we report the development of a reliable and efficient protocol for rapid in vitro shoot induction and rooting of pecan plants by culturing nodal explants from seed in liquid culture. Although micropropagation of pecan through nodal explants from seed will result in heterozygous plants, the protocol optimized in this study could be applied for producing true-type plants from mature tissues.The unshelled mature seeds of pecan cultivars 'Cape Fear' and 'Desirable' were washed with sterile water for 10 min and then sterilized by immersion in the carbendazim solution (1.0 g l Ϫ1; Sigma Chemical Co., St. Louis, MO, USA) overnight. The seeds were washed with sterile water and treated with 70% ethanol for 30 min., again washed with sterile water for three times to remove ethanol. Subsequently, the seeds were treated with a 2.83% (w/v) sodium hypochlorite for 30 min. The seeds were thoroughly rinsed five times with sterile water and aseptically shelled using nut cracker. The embryos were aseptically isolated from the seeds and treated with 2.83% (w/v) sodium hypochlorite solution for 10 min. and rinsed with sterilized water five times for five min Abstract An efficient method for in vitro propagation has been developed for pecan [Carya illinoinensis (Wangenh) K. Koch], a hig...

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Role of carbohydrates in micropropagation of cork oak

Cited by 71 publications

References 26 publications

An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

An efficient micropropagation system for Eclipta, alba—A valuable medicinal herb

In vitro propagation of cherry birch (Betula lentaL.)

In vitro propagation of pecan [Carya illinoinensis (Wangenh) K. Koch]

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