Role of c-Abl in Ang II-induced aortic dissection formation: Potential regulatory efficacy on phenotypic transformation and apoptosis of VSMCs

Zhou, Xianwu; Cheng, Jiancheng; Chen, Zerui; Li, Huadong; Chen, Shu; Xu, Fei; Fan, Ruixin; Zhuang, Jian; Sun, Tucheng

doi:10.1016/j.lfs.2020.117882

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…Studies have shown that MAPK1 is upregulated by miR-140-3p and inhibits CAD cell apoptosis (33). Knockout of ABL1 gene inhibits c-Abl activity and significantly reduces apoptosis of VSMCs and synthetic phenotypic transformation induced by Ang II both in vivo and in vitro (34). CRK plays a key role in Rac1-induced membrane ruffling and Rap1-mediated nascent focal complex stabilization, which contributed to ephrin-B1induced human aortic endothelial cells migration (35).…”

Section: Discussionmentioning

confidence: 99%

Role of competitive endogenous RNA networks in the pathogenesis of coronary artery disease

Zuo¹,

Xu²,

Wang³

et al. 2021

Ann Transl Med

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Background: The present study aimed to construct a network of competitive endogenous RNAs (ceRNAs) related to the pathogenesis of coronary artery disease (CAD), to provide a novel rationale for CAD treatment.Methods: Bioinformatics methods were applied to screen for differentially expressed long non-coding RNAs (DElncRNAs), microRNAs (DEmiRNAs), and mRNAs (DEmRNAs) from the GSE68506, GSE59421, and GSE20129 datasets of the Gene Expression Omnibus (GEO) database. The miRcode database was used to predict lncRNA-binding miRNAs. The miRTarBase, miRDB, and TargetScan databases were used to predict the target genes of these miRNAs. An mRNA-miRNA-lncRNA ceRNA network of CAD was established.Results: Between the CAD and normal control groups there were 264 DElncRNAs, 106 DEmiRNAs, and 1,879 DEmRNAs. We screened these differentially expressed gens (DEGs) respectively. There were 21 DElncRNAs, 13 DEmiRNAs, and 143 DEmRNAs in the ceRNA network by using Cytoscape application. The DEmRNAs were involved in the PI3K-Akt signaling pathway and the NF-κB signaling pathway. The key genes in the protein-protein interaction (PPI) network were HSP90AA1, CDKN1A,

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Section: Discussionmentioning

confidence: 99%

Role of competitive endogenous RNA networks in the pathogenesis of coronary artery disease

Zuo¹,

Xu²,

Wang³

et al. 2021

Ann Transl Med

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“…miRNAs have been revealed to play a key part in all biological processes, and aberrant miRNA expression has been linked to a variety of diseases, including neurodegenerative diseases, metabolic disorders, cancer, and cardiovascular diseases (28,29). miRNAs are also involved in the proliferation, apoptosis, migration, and phenotype conversion of VSMCs (22). For example, researchers found that miRNA-22 inhibits vascular smooth muscle cell apoptosis in AD vascular remodeling by targeting p38 mitogenactivated protein kinase α (p38MAPKα) (30).…”

Section: Micrornas In Aortic Dissectionmentioning

confidence: 99%

“…Studies have found that ncRNA is a key regulator of gene expression under physiological and pathological conditions and interacts with DNA, RNA, and proteins (21). In molecular biology, miRNAs are involved in the proliferation, apoptosis, migration, and phenotypic transition of VSMCs (22). Therefore, an increasing number of studies on the pathogenesis of AD have begun to focus on miRNAs.…”

Section: Introductionmentioning

confidence: 99%

Non-coding RNAs Regulate the Pathogenesis of Aortic Dissection

Cheng

et al. 2022

Front. Cardiovasc. Med.

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Aortic dissection (AD) is a fatal cardiovascular disease. It is caused by a rupture of the aortic intima or bleeding of the aortic wall that leads to the separation of different aortic wall layers. Patients with untreated AD have a mortality rate of 1–2% per hour after symptom onset. Therefore, effective biomarkers and therapeutic targets are needed to reduce AD-associated mortality. With the development of molecular technology, researchers have begun to explore the pathogenesis of AD at gene and protein levels, and have made some progress, but the pathogenesis of AD remains unclear. Non-coding RNAs, such as microRNAs, lncRNAs, and circRNAs, have been identified as basic regulators of gene expression and are found to play a key role in the pathogenesis of AD. Thus, providing a theoretical basis for developing these non-coding RNAs as clinical biomarkers and new therapeutic targets for AD in the future. Previous studies on the pathogenesis of AD focused on miRNAs, but recently, there have been an increasing number of studies that explore the role of lncRNAs, and circRNAs in AD. This review summarizes the existing knowledge on the roles of various non-coding RNAs in the pathogenesis of AD, discusses their potential role as clinical biomarkers and therapeutic targets, states the limitations of existing evidence, and recommends future avenues of research on the pathogenesis of AD.

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“…A total of 22 studies enrolling 701 mice were included in this study 8,[11][12][13][14][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] . 449 mice were treated with an AngII pump, and 252 mice were treated with a saline pump.…”

Section: Meta-analysis Study Characteristicsmentioning

confidence: 99%

Establishment of a meta-analysis based novel aortic dissection mouse model

Jiang

Liu

et al. 2022

Sci Rep

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Aortic dissection (AD) is a life-threatening disease and the detailed mechanism remains unclear. Thus, proper animal models are urgently required to better understand its pathogenesis. Our current study aims to establish a reliable, time and cost-effective mouse AD model. To conduct the meta-analysis, we searched PubMed for related studies up to 2021 and statistical analysis was conducted using Review Manager 5.4. For the animal experiment, 6-week-old male ApoE−/− mice were given β-aminopropionitrile (BAPN) at a concentration of 1 g/L for 3 weeks before being infused with saline, 1000 ng/kg/min or 2500 ng/kg/min angiotensin II (AngII) via osmotic mini pumps for 2 or 4 weeks. To determine the presence of AD, we performed B-ultrasonography, hematoxylin and eosin (H&E) staining, and van Gieson staining. The result of the meta-analysis showed that the use of BAPN and more than 2000 ng/kg/min AngII can increase the rate of AD formation, whereas administrating Ang II for more than 28 days has no significant effect on the rate of AD formation when compared with the less than 14 days group. In the present study, mice treated with BAPN combined with 2500 ng/kg/min AngII for 2 weeks (12/20) had a significantly higher AD formation rate than mice treated with BAPN combined with 1000 ng/kg/min Ang II for 4 weeks (2/10), and had a similar model formation rate compared with the mice treated withβ-aminopropionitrile combined with 2500 ng/kg/min AngII for 4 weeks (6/10). There were 3 mice (3/10) and 6 mice (6/20) who died in the group treated with β-aminopropionitrile combined with 2500 ng/kg/min AngII for 4 weeks and 2 weeks respectively, and only one mouse (1/10) died in the group treated with β-aminopropionitrile combined with 1000 ng/kg/min AngII for 4 weeks. In 6-week-old male ApoE−/− mice that received with 1 g/L BAPN in the drinking water for 3 weeks along with 2500 ng/kg/min AngII infusion via osmotic mini pumps for 2 weeks, the highest model formation rate and relative lower cumulative mortality were noted.

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Role of c-Abl in Ang II-induced aortic dissection formation: Potential regulatory efficacy on phenotypic transformation and apoptosis of VSMCs

Cited by 8 publications

References 34 publications

Role of competitive endogenous RNA networks in the pathogenesis of coronary artery disease

Role of competitive endogenous RNA networks in the pathogenesis of coronary artery disease

Non-coding RNAs Regulate the Pathogenesis of Aortic Dissection

Establishment of a meta-analysis based novel aortic dissection mouse model

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