Role of bone marrow‐derived progenitor cells in the maintenance and regeneration of dental mesenchymal tissues

Zhou, Jing; Shi, Songtao; Shi, Yuanyuan; Xie, Han; Chen, Lei; He, Yueqiu; Guo, Weihua; Wen, Lu; Jin, Yan

doi:10.1002/jcp.22538

Cited by 57 publications

(56 citation statements)

References 40 publications

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“…All procedures of cell culture and identification, of both humans and minipigs, were the same as the previous description71252.…”

Section: Methodsmentioning

confidence: 99%

“…However, the periodontium, a complex composed of periodontal ligament (PDL), cementum, alveolar bone and gingiva, is difficult to regenerate integrally. Periodontal ligament stem cells (PDLSCs) and iliac bone-derived MSCs (IBMSCs) were reported to participate in periodontium tissue regeneration10111213, and their interaction also facilitated this process14. Our previous study proved that compound cell aggregates (CAs) of PDLSCs and bone marrow MSCs promoted periodontium regeneration with regenerative microenvironment reconstruction15.…”

mentioning

confidence: 99%

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RETRACTED ARTICLE: Jawbone microenvironment promotes periodontium regeneration by regulating the function of periodontal ligament stem cells

Zhu¹,

Liu²,

Liu³

et al. 2017

Sci Rep

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During tooth development, the jawbone interacts with dental germ and provides the development microenvironment. Jawbone-derived mesenchymal stem cells (JBMSCs) maintain this microenvironment for root and periodontium development. However, the effect of the jawbone microenvironment on periodontium tissue regeneration is largely elusive. Our previous study showed that cell aggregates (CAs) of bone marrow mesenchymal stem cells promoted periodontium regeneration on the treated dentin scaffold. Here, we found that JBMSCs enhanced not only the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) but also their adhesion to titanium (Ti) material surface. Importantly, the compound CAs of PDLSCs and JBMSCs regenerated periodontal ligament-like fibers and mineralized matrix on the Ti scaffold surface, both in nude mice ectopic and minipig orthotopic transplantations. Our data revealed that an effective regenerative microenvironment, reconstructed by JBMSCs, promoted periodontium regeneration by regulating PDLSCs function on the Ti material.

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“…All procedures of cell culture and identification, of both humans and minipigs, were the same as the previous description71252.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

RETRACTED ARTICLE: Jawbone microenvironment promotes periodontium regeneration by regulating the function of periodontal ligament stem cells

Zhu¹,

Liu²,

Liu³

et al. 2017

Sci Rep

Self Cite

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“…For the first question, a recent report may shed some light on the possibility. Zhou et al (2011) utilized BMMSCs from GFP (green fluorescent protein) transgenic mice and transfused them via intravenous injection into irradiated wild-type mice. They found that these transplanted GFP + BMMSCs migrated to and resided in pulp and periodontal ligament.…”

Section: Cell Homingmentioning

confidence: 99%

Missing Concepts inDe NovoPulp Regeneration

Huang

Garcı́a-Godoy

2014

J Dent Res

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Regenerative endodontics has gained much attention in the past decade because it offers an alternative approach in treating endodontically involved teeth. Instead of filling the canal space with artificial materials, it attempts to fill the canal with vital tissues. The objective of regeneration is to regain the tissue and restore its function to the original state. In terms of pulp regeneration, a clinical protocol that intends to reestablish pulp/dentin tissues in the canal space has been developed-termed revitalization or revascularization. Histologic studies from animal and human teeth receiving revitalization have shown that pulp regeneration is difficult to achieve. In tissue engineering, there are 2 approaches to regeneration tissues: cell based and cell free. The former involves transplanting exogenous cells into the host, and the latter does not. Revitalization belongs to the latter approach. A number of crucial concepts have not been well discussed, noted, or understood in the field of regenerative endodontics in terms of pulp/dentin regeneration: (1) critical size defect of dentin and pulp, (2) cell lineage commitment to odontoblasts, (3) regeneration vs. repair, and (4) hurdles of cell-based pulp regeneration for clinical applications. This review article elaborates on these missing concepts and analyzes them at their cellular and molecular levels, which will in part explain why the non-cell-based revitalization procedure is difficult to establish pulp/dentin regeneration. Although the cell-based approach has been proven to regenerate pulp/dentin, such an approach will face barriers-with the key hurdle being the shortage of the current good manufacturing practice facilities, discussed herein.

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“…9 hMSCs are promising key candidates for periodontal regeneration purposes. 5,7,20,21 Thus, our study has investigated for the first time the interplay of hMSCs with different oral cell types by means of an innovative in vitro approach, based on interactive cocultures facilitating physiologically relevant nondirect contact-based cell-to-cell interactions, also occurring in vivo.…”

mentioning

confidence: 99%

Crosstalk on Cell Behavior in Interactive Cocultures of hMSCs with Various Oral Cell Types

Proksch

Steinberg

Stampf

et al. 2012

Tissue Engineering Part A

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When prospectively applied for regenerative therapies, human bone-marrow-derived mesenchymal stem cells (hMSCs) interact with the locally residing host cells. With respect to the developmentally particular origin of oral cells, little is known about the putatively discriminative behavioral responses of hMSCs in interaction with various oral cell types, including human alveolar bone osteoblasts (hOAs), periodontal ligament fibroblasts (hPDLs), and gingival fibroblasts (hGFs). To assess the crosstalk between hMSCs and oral cells, interactive cocultures were established by combining well-characterized hMSCs with hOAs, hPDLs, or hGFs, and the behavioral hMSC aspects, that is, proliferation and gene expression, were measured by employing a 5-bromo-2'-deoxyuridine assay and real-time polymerase chain reaction, while apoptosis was quantified by in situ cell death detection kit. hMSCs expressed the typical antigen spectrum lacking CD34, CD45, CD14, CD19, and HLA-DR, while expressing CD73, CD90, and CD105, and could successfully be transformed into adipocytes, osteocytes, and chondrocytes. Monocultured control hMSCs proliferated readily, whereas a general reduction of BrdU-labeled cells was observed in cocultures. Globally, upon extending time periods, interactive coculture combinations of hMSCs with hOAs reduced both osteogenic gene and stem cell marker transcription in hMSCs, a phenomenon appearing less pronounced by combining hMSCs with hPDLs, such that the observed effects in terms of proliferation and gene expression followed the same ranking: hOAs>hGFs>hPDLs. Vice versa, in interactive hMSC cocultures, the cell survival rate was significantly increased, irrespective from the combined coculture cell counterpart. Our results show for the first time that behavior of hMSCs reflected by proliferation and gene expression was governed by interaction with various oral cells in a cell-type-discriminative manner. In addition, hMSC coculture restrains apoptosis, such that influences on cell behavior appear as a crosstalk. In summary, interactive cocultures render the basis for a prospective prediction of mutual cell behavior in hMSC-based oral tissue regeneration disclosing that oral cells shift hMSC behavior from proliferation to differentiation and apoptosis-repressing features.

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Role of bone marrow‐derived progenitor cells in the maintenance and regeneration of dental mesenchymal tissues

Cited by 57 publications

References 40 publications

RETRACTED ARTICLE: Jawbone microenvironment promotes periodontium regeneration by regulating the function of periodontal ligament stem cells

RETRACTED ARTICLE: Jawbone microenvironment promotes periodontium regeneration by regulating the function of periodontal ligament stem cells

Missing Concepts inDe NovoPulp Regeneration

Crosstalk on Cell Behavior in Interactive Cocultures of hMSCs with Various Oral Cell Types

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