Role of Blocking Serum Factors in Organ Grafted Rats

Abstract

The aim of the present study was to investigate whether inhibition of the mixed lymphocyte culture (M.L.C.) interaction by sera from organ transplanted rats could be used as an indicator of the development of immunological enhancement. The findings may be summarized as follows: 1) Conventionally produced immune sera exhibited an antigen‐specific inhibition of M.L.C. whether directed against the stimulating or the responding cells. 2) Sera from organ transplanted rats also consistently inhibited the M.L.C. interaction. However, no difference was found between sera from rats who had rejected their grafts and from recipients of long‐term surviving grafts. 3) Sera from long‐term survivors and post rejection sera proved equally capable of inducing passive enhancement in vivo.

Inhibition of the Mixed Leucocyte Culture Reaction by Plasma from Renal Transplant Recipients

“…Sengar et al (1973) suggested a correlation between the presence of MLC blocking factor and long-term renal allograft survival in humans. However, Heron & Ssrensen (1973) observed no difference in the MLC inhibitory capacity of sera obtained either from rats who had rejected their grafts or from recipients of long-term surviving allografts. I n the present investigation, the MLC blocking system failed to distinguish between the action of plasma from recipients who had rejected the allo-grafts and from recipients showing longterm allograft acceptance.…”