Role of biotechnology in the conservation of rare, threatened and endangered medicinal plant species in the Kingdom of Eswatini (Swaziland)

Kunene, Emmanuel N.; Masarirambi, Michael T.

doi:10.30918/ampr.63.18.015

Cited by 6 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Micro-propagation protocols need to be established for all the endangered and threatened plant species. To reduce the depletion of the medicinal plant genetic resource, the coverage strategy of sustainable harvesting, traditional propagation methods, and plant biotechnology should be employed (Kunene and Masarirambi 2018). 13C composition and wood morphology between AD 1375 and 1995.…”

Section: Conservation Concernsmentioning

confidence: 99%

Phytochemical constituents and pharmacological activities of Breonadia salicina (Vahl) Hepper and J.R.I.Wood (Rubiaceae)

et al. 2022

View full text Add to dashboard Cite

Background Medicinal plants have been the mainstay for the treatment of various diseases since antiquity. The importance of ethnomedicinal plants in drug discovery and development can never be overemphasized. Breonadia salicina (Vahl) Hepper and J.R.I.Wood (Rubiaceae) is a medium to a large plant that is widely distributed in the tropical and subtropical regions; the leaf, stem bark, and the root have been used in folk medicine for the treatment of cancer, arthritis, inflammation, wound infections, fever, diarrhoea, and vomiting. Studies showed that the plant contains several phytochemical compounds, some of which were isolated in their pure form from various parts of the plant. The compounds isolated include ursolic acid, α-amyrin, stigmasterol, and sitosterol. Other essential compounds isolated were 7-(β-d-apiofuranosyl-(1 → 6)-β-d-glucopyranosyl) umbelliferone (Adicardin), 7-hydroxycoumarin, and 6-hydroxy-7-methoxycoumarin. Literature works on B. salicina are limited, and information regarding its nutritional value is lacking. However, the leaf of the plant is used as food preservative. Main body This review is a compilation of information obtained from scholarly databases including ScienceDirect, ResearchGate, Sci-Hub, Wiley Online Library, and Google Scholar using search keywords related to the topic of the review. The articles were selected based on the year of publication, which was from 2010 to 2021, but some older references were still be included in this review because some data in recent articles were cited from older sources. This review focuses on the ethnomedicinal uses of this plant as well as the underpinning experimental evidence of its pharmacological activities such as antibacterial, antifungal, and anti-trypanasomal activities. Conclusion There is a need to explore the potentials of this plant by initially isolating and characterizing the bioactive compounds and then subsequently evaluating its various pharmacological activities and be considered for further development to a novel therapeutic compound.

show abstract

Section: Conservation Concernsmentioning

confidence: 99%

Phytochemical constituents and pharmacological activities of Breonadia salicina (Vahl) Hepper and J.R.I.Wood (Rubiaceae)

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Another technique of domesticating the indigenous fruit and nut trees is the use of plant biotechnological tools i.e. in vitro propagation [89,90]. In the modern age of science, plant tissue culture is an indispensable part of biotechnology [91].…”

Section: Domestication Of Indigenous Fruit and Nut Treesmentioning

confidence: 99%

Domesticating and Commercialisation of Indigenous Fruit and Nut Tree Crops for Food Security and Income Generation in the Kingdom of Eswatini

Kunene

Nxumalo

Ngwenya

et al. 2020

CJAST

View full text Add to dashboard Cite

Indigenous fruit and nut tree crops are very important to the human diet and subsequent food security and income generation in the Kingdom of Eswatini. Indigenous fruits and nuts are important for the nutritional contribution as sources of Vitamins and minerals. They also provide fibre which is essential for proper digestion of food. Fruits, particularly nuts provide man with unsaturated fats which are good for the heat and the cardio vascular system. Indigenous fruits and nuts are aesthetic as they provide interesting colour, texture and variety. Despite the immense benefits of indigenous fruits and nuts to the people of the Kingdom of Eswatini, there has not been enough effort put towards domesticating the indigenous fruit and nut trees and subsequent commercialising their products. The objective of this paper was to document strategies towards domestication and commercialisation of indigenous fruits and nuts for improved livelihoods of Emaswati citizens, which in turn will help achieve sustainable development goals (SDGs) pertaining to human health and food security in the Kingdom of Eswatini. It can be concluded that domesticating indigenous fruit and nut trees can be achieved through a multi-stakeholder involvement. Domestication can also help in conservation of indigenous fruit and nut trees. Value addition on indigenous fruits and nuts can generate more income.

show abstract

“…Thus, to meet the increasing demand of T. indica, it is mandatory to develop alternative methods having high multiplication rates which will substantially reduce the dependence on the wild populations. In this context, In vitro propagation using advanced biotechnological tools can be highly beneficial to meet the rising demand of this herb in future [26]. Earlier, the micropropagation of T. indica has been reported using different explants through axillary shoot proliferation nodal explants and de novo adventitious shoot formation either directly from the explants or indirectly through the callus [25,27,28,29,30,31].…”

Section: Plos Onementioning

confidence: 99%

Anti-neuroinflammatory potential of Tylophora indica (Burm. f) Merrill and development of an efficient in vitro propagation system for its clinical use

et al. 2020

View full text Add to dashboard Cite

Neuroinflammation is a major risk factor associated with the pathogenesis of neurodegenerative diseases. Conventional non-steroidal anti-inflammatory drugs are prescribed but their long term use is associated with adverse effects. Thus, herbal based medicines are attracting major attraction worldwide as potential therapeutic candidates. Tylophora indica (Burm. f) Merrill is a valuable medicinal plant well known in Ayurvedic practices for its immunomodulatory, anti-oxidant, anti-asthmatic and antirheumatic activities. The present study aimed to elucidate the antineuroinflammatory potential of water and hydroalcoholic leaf extracts of micropropagated plants of T. indica using BV-2 microglia activated with lipopolysaccharide as an in vitro model system and development of an efficient reproducible protocol for its in vitro cloning. Non cytotoxic doses of the water and hydroalcoholic extracts (0.2μg/ml and 20μg/ml, respectively) were selected using MTT assay. α-Tubulin, Iba-1 and inflammatory cascade proteins like NFκB, AP1 expression was studied using immunostaining to ascertain the anti-neuroinflammatory potential of these extracts. Further, anti-migratory activity was also analyzed by Wound Scratch Assay. Both extracts effectively attenuated lipopolysaccharide induced microglial activation, migration and the production of nitrite via regulation of the expression of NFκB and AP1 as the possible underlying target molecules. An efficient and reproducible protocol for in vitro cloning of T. indica through multiple shoot proliferation from nodal segments was established on both solid and liquid Murashige and Skoog's (MS) media supplemented with 15μM and 10μM of Benzyl Amino Purine respectively. Regenerated shoots were rooted on both solid and liquid MS media supplemented with Indole-3-butyric acid (5-15μM) and the rooted plantlets were successfully acclimatized and transferred to open field conditions showing 90% survivability. The present study suggests that T. indica may prove to be a potential anti-neuroinflammatory agent and may be further explored as a potential therapeutic candidate for the management of neurodegenerative diseases. Further, the current study will expedite the conservation of T. indica ensuring ample supply of this threatened medicinal plant to fulfill its increasing demand in herbal industry.

show abstract

Role of biotechnology in the conservation of rare, threatened and endangered medicinal plant species in the Kingdom of Eswatini (Swaziland)

Cited by 6 publications

References 17 publications

Phytochemical constituents and pharmacological activities of Breonadia salicina (Vahl) Hepper and J.R.I.Wood (Rubiaceae)

Phytochemical constituents and pharmacological activities of Breonadia salicina (Vahl) Hepper and J.R.I.Wood (Rubiaceae)

Domesticating and Commercialisation of Indigenous Fruit and Nut Tree Crops for Food Security and Income Generation in the Kingdom of Eswatini

Anti-neuroinflammatory potential of Tylophora indica (Burm. f) Merrill and development of an efficient in vitro propagation system for its clinical use

Contact Info

Product

Resources

About