Role of Biosynthesized Ag-NPs Using Aspergillus niger (MK503444.1) in Antimicrobial, Anti-Cancer and Anti-Angiogenic Activities

Abstract

Green synthesis of nanoparticles is regarded as a safe and non-toxic process over conventional synthesis. Owing to the medicinal value of biologically derived biomolecules and utilizing them in synergy with nanoscience to offer more accurate therapeutic options to various diseases is an emerging field. One such study we present here with highlights of the synthesis and efficacy of biogenic silver nanoparticles produced from the extract of Aspergillus niger SAP2211 (accession number: MK503444.1) as an antimicrobial, anti-cancerous and anti-angiogenic agent. The synthesized Ag-NPs were characterized following UV–vis, FTIR, XRD, SEM and TEM, and were found to possess bactericidal activity against the selected pathogenic microbes, such as Staphylococcus aureus, Escherichia coli, and Salmonella typhi. Further, we evaluated cytotoxicity effect of this biogenic Ag-NPs using MMT assay on normal cardio myoblast (H9C2) and cancerous human cervical carcinoma (HeLa) cells. Doxorubicin used as positive control. This Ag-NPs have shown trivial cytotoxicity at the IC50 concentration on normal cells (IC50 = 47.17 µg/ml) over the cancer cells (IC50 = 8.609 µg/ml) with nearly 7 fold difference, indicating it as a selective anti-cancerous agent in contrast to standard drug doxorubicin (IC50 = 6.338 µg/ml). Further in-vitro assessment of wound healing capability by scratch wound healing assay, invasion by transwell matrigel invasion assay, and apoptosis via DAPI and annexin V-FITC assays were studied in HeLa cells. Synthesized biogenic Ag-NPs have shown to be anti-angiogenic in nature, which was established by in-vivo chick chorioallantois membrane assay. Overall, in vitro studies revealed that biogenic Ag-NPs positively inhibited migration, invasion, and induced apoptosis, and in-vivo CAM assay revealed that intercapillary network was reduced and the angiogenesis was inhibited.

Advances in Nanotechnology as a Potential Alternative for Plant Viral Disease Management

“…Nanomaterials can be used in the field of plant protection as a protectant or as a carrier for precise and targeted delivery via adsorption, encapsulation, or conjugation with active ingredients of pesticides. Nanomaterials can be applied as a foliar spray in seed treatment and root application, as they are known to possess antifungal, antibacterial, and antiviral properties (Wong and Liu, 2010 ; Krishnaraj et al, 2012 ; Elbeshehy et al, 2015 ; Pasha et al, 2022 ). The NPs exhibiting plant disease suppressing activity include nonmetals, metalloids, metallic oxides, and carbon nanomaterials (Elmer et al, 2018 ).…”

Cd stress alleviation in mung-bean seedlings with biogenic hydroxyapatite nanoparticles as ecofriendly remediation agents

“…In previous studies, these biomolecules have worked as biocatalysts, bioreductants, biocapping agents, and biostabilizers in the fabrication of diverse NPs, such as Ag, MgO, CeO 2 and ZnO. [44][45][46][47] Based on the understanding of HAP mineralization, the abundance of biomolecules and mild pH conditions of A. niger broth may provide a facile matrix for the biological synthesis of HAP NPs. Therefore, the three aims of this study are as follows: (1) to prepare biogenic HAP NPs employing A. niger broth as the culture medium and characterize the properties of the as-obtained biogenic HAP NPs; (2) to analyze the remediation effect of HAP NPs by determining the Cd distribution in mung bean (Vigna radiata) seedlings and details of plant development under Cd stress, including growth parameters, physiological characteristics, and cellular oxidative levels; and (3) to explore the mechanism of biogenic HAP NPs as ecofriendly remediation agents for Cd pollution in agriculture.…”

Synthesis of Nano Sulfur/Chitosan-Copper Complex and Its Nematicidal Effect against Meloidogyne incognita In Vitro and on Coffee Pots

“…Today, nanotechnology has been applied in many fields including medicine, energy, electronics, and agriculture ( Wypij et al, 2023 ). Nanomaterials, particularly nanocomposites have been used in agriculture as a plant protection agent because of their antibacterial, antifungal, and antiviral properties ( Elbeshehy et al, 2015 ; Krishnaraj et al, 2012 ; Mishra et al, 2018 ; Pasha et al, 2022 ; Wong and Liu, 2010 ), and/or nano-nutrient fertilizer properties ( Du et al, 2019 ; Tomadoni et al, 2019 ). Nanoparticles capable of inhibiting plant diseases are metal, metal oxide, metalloid, and carbon nanoparticles ( Elmer et al, 2018 ).…”