Role of Biomechanical Forces in Stem Cell Vascular Lineage Differentiation

Potter, Claire M.F.; Lao, Ka Hou; Zeng, Lingfang; Xu, Qingbo

doi:10.1161/atvbaha.114.303423

Cited by 56 publications

(38 citation statements)

References 80 publications

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“…For instance, it is reported that shear stress promotes maturation of megakaryocytes . Moderate shear stress was found to have an influence on stem cell differentiation . Excessive shear stress, in contrast, even dispatches cells by disrupting the membrane.…”

Section: Methodsmentioning

confidence: 99%

Controlling Shear Stress in 3D Bioprinting is a Key Factor to Balance Printing Resolution and Stem Cell Integrity

Blaeser

Campos

Puster

et al. 2015

Adv Healthcare Materials

610

568

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in cell biology, [ 29,30 ] e.g., in cell signaling and protein expression. [31][32][33] For instance, it is reported that shear stress promotes maturation of megakaryocytes. [ 34 ] Moderate shear stress was found to have an infl uence on stem cell differentiation. [ 35 ] Excessive shear stress, in contrast, even dispatches cells by disrupting the membrane. These phenomena are even more crucial in bioprinting processes, where hydrogels of high viscosity and small nozzles are applied in an attempt to improve the fi nal printing resolution. Here, we show that both hydrogel viscosity and nozzle size directly affect shear stress. To prevent adverse cell response and printing-related cell death, it is essential to control the shear stress level, identify its most important drivers, and study the cell response upon different stress levels. We hypothesize that regulating shear stress and elucidating its impact would be of great use in balancing cell integrity and printing resolution.We present a microvalve-based bioprinting system for the manufacturing of high resolution, multi-material 3D structures ( Scheme 1 A). Applying a straightforward fl uid-dynamics model, we were able to precisely control shear stress at the nozzle site, which could be adjusted by varying the printing pressure, hydrogel viscosity, and the nozzle diameter. Using this system, we conducted a broad study on how cell viability and proliferation potential are affected by different levels of shear stress (Scheme 1 B). Generating complex, multi-material 3D-structures, we demonstrate that high-resolution printing at moderate, cell-friendly nozzle shear stress are not mutually exclusive.The printer used throughout this study comprised four microvalve-based print heads, each individually controllable and heatable, mounted to a three-axis robotic system ( Figure S1, Supporting Information). A metal stage that could be lowered into a container fi lled with a bi-phasic support liquid-perfl uorocarbon (PFC) and an aqueous crosslinker solution-was used as a printing platform that allowed for the manufacturing of macroscopic, multi-layered 3D structures ( Figure S1, Supporting Information). The presented printing system dispenses single drops of cell-hydrogel suspension by jetting using electromagnetic microvalves. Thus, cells are primarily exposed to mechanical stress in the form of shear stress. To describe the shear stress condition in the nozzle of the valve, we developed a fl uid dynamics model for transient fl ow of non-Newtonian fl uids (hydrogels) based on the Bernoulli equation for unsteady fl ow: Equation (

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Section: Methodsmentioning

confidence: 99%

Controlling Shear Stress in 3D Bioprinting is a Key Factor to Balance Printing Resolution and Stem Cell Integrity

Blaeser

Campos

Puster

et al. 2015

Adv Healthcare Materials

610

568

View full text Add to dashboard Cite

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“…Mechanical stimulation also plays important roles in regulating the differentiation directions of MSCs. Fluid shear stress can induce endothelial differentiation of multiple kinds of stem cells . Bai et al.…”

Section: Mechanisms Of Msc Utilization For Vascular Regenerationmentioning

confidence: 99%

Mesenchymal stem cells and vascular regeneration

Hong

Potter

et al. 2017

Microcirculation

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In recent years, MSCs have emerged as a promising therapeutic cell type in regenerative medicine. They hold great promise for treating cardiovascular diseases, such as myocardial infarction and limb ischemia. MSCs may be utilized in both cell-based therapy and vascular graft engineering to restore vascular function, thereby providing therapeutic benefits to patients. The efficacy of MSCs lies in their multipotent differentiation ability toward vascular smooth muscle cells, endothelial cells and other cell types, as well as their capacity to secrete various trophic factors, which are potent in promoting angiogenesis, inhibiting apoptosis and modulating immunoreaction.Increasing our understanding of the mechanisms of MSC involvement in vascular regeneration will be beneficial in boosting present therapeutic approaches and developing novel ones to treat cardiovascular diseases. In this review, we aim to summarize current progress in characterizing the in vivo identity of MSCs, to discuss mechanisms involved in cell-based therapy utilizing MSCs, and to explore current and future strategies for vascular regeneration. K E Y W O R D Sangiogenesis, mesenchymal stem cells, stem cell-based therapy, vascular regeneration

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“…We speculate that diabetes contributes to the vascular smooth muscle cell (VSMC) phenotype. VSMCs can perform both contractile and synthetic functions, which are associated with and characterized by changes in morphology, proliferation, and migration rates and the expression of different marker proteins [22–24]. Diabetes-induced vascular complications are associated with VSMC phenotypic modulation, which involves switching from a contractile to a synthetic-proliferative phenotype [25].…”

Section: Discussionmentioning

confidence: 99%

Parallel comparison of risk factors between progression of organic stenosis in the coronary arteries and onset of acute coronary syndrome by covariance structure analysis

et al. 2017

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BackgroundIt is widely accepted that progression of organic stenosis in the coronary arteries and onset of acute coronary syndrome (ACS) are similar in the development of atherosclerosis. However, the extent of the association of each risk factor with the respective pathological conditions has not been fully elucidated.ObjectivesWe investigated the differences in risk factors between these conditions using a statistical procedure.MethodsThe study population consisted of 1,029 patients with ischemic heart disease (IHD). We divided the study population into two groups (ACS and non-ACS) and by diseased vessels (organic stenosis). Covariance structure analysis was simultaneously performed in one equation model for determination and comparison of the risk factors for organic stenosis and ACS.ResultsThe analysis revealed that age (standardized regression coefficient, β: 0.206, P < 0.001), male gender (β: 0.126, P < 0.001), HbA1c level (β: 0.109, P < 0.001), HDL level (β: -0.109, P < 0.001) and LDL level (β: 0.127, P = 0.002) were significant for the advancement of organic stenosis. HDL level (β: 0100, P = 0.002) and MDA-LDL level (β: 0.335, P < 0.001) were significant for the onset of ACS, but age, HbA1c and LDL (P = NS or β < 0.1, respectively) were not. Among the risk factors, age, HbA1c and LDL were significantly more strongly associated with organic stenosis than ACS, while MDA-LDL was significantly more strongly associated with ACS than organic stenosis.ConclusionsThe current statistical analysis revealed clear differences among the risk factors between the progression of organic stenosis and the onset of ACS. Among them, the MDA-LDL level should be considered to indicate a substantial risk of ACS.

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Role of Biomechanical Forces in Stem Cell Vascular Lineage Differentiation

Cited by 56 publications

References 80 publications

Controlling Shear Stress in 3D Bioprinting is a Key Factor to Balance Printing Resolution and Stem Cell Integrity

Controlling Shear Stress in 3D Bioprinting is a Key Factor to Balance Printing Resolution and Stem Cell Integrity

Mesenchymal stem cells and vascular regeneration

Parallel comparison of risk factors between progression of organic stenosis in the coronary arteries and onset of acute coronary syndrome by covariance structure analysis

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