Role of biological matrices during the analysis of chiral drugs by liquid chromatography

Mislanova, Csilla; Hutta, Milan

doi:10.1016/j.jchromb.2003.07.018

Cited by 42 publications

(24 citation statements)

References 170 publications

(187 reference statements)

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“…This problem is analyte-specific and can potentially be resolved by using a more hydrophobic column in the second dimension, optimization of mobile phase [15], tight control of dead volume between columns [3,14], or even operating the second-dimension column (and trapping of the peak heart-cut) at low temperature [16]. Another strategy to avoid peak dispersion is to re-focus the analyte in the second dimension by the simultaneous addition of a mobile phase low organic content to the analyte peak heart-cut, resulting in improved adsorption of diluted analyte onto the second RP column [6].…”

Section: Overview Of 2-d Rp-rp Applicationsmentioning

confidence: 99%

Two‐dimensional reverse phase‐reverse phase chromatography: A simple and robust platform for sensitive quantitative analysis of peptides by LC/MS. Hardware design

Rogatsky

Stein

2006

J of Separation Science

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We have revised current two-dimensional RP-RP approaches and developed a new robust 2-D RP-RP platform. This platform was implemented on an Agilent 1100 2-D liquid chromatography system and is based on high pressure switching between two high-resolution RP columns. An independent binary gradient was implemented for each dimension. The powerful combination of dual analytical columns with independent gradient elution achieves high analyte purity, effectively eliminates matrix effects, and maximizes MS sensitivity in Q1 SIM comparable to the sensitivity enhancements of MS/MS-based methods. Implementation of dual simultaneous gradient profiles (overlapped gradients) reduces 2-D method run-time to the scale of 1-D method run-times. This robust and sensitive approach is particularly suitable for hydrophobic peptides and small proteins and can be used as a routine standard technique for enhanced on-line peptide purification coupled with mass spectrometric detection.

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Section: Overview Of 2-d Rp-rp Applicationsmentioning

confidence: 99%

Two‐dimensional reverse phase‐reverse phase chromatography: A simple and robust platform for sensitive quantitative analysis of peptides by LC/MS. Hardware design

Rogatsky

Stein

2006

J of Separation Science

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“…Chiral separations can be classified into three categories [15]. The first one is pre-column derivatization with a chiral derivatization reagent to generate diastereomers; the second one involves adding a chiral agent to the mobile phase to form adducts with the enantiomers; and the third one is a direct method using chiral stationary phase [20] [25].…”

Section: Introductionmentioning

confidence: 99%

Fast and Sensitive Chiral Analysis of Amphetamines and Cathinones in Equine Urine and Plasma Using Liquid Chromatography Tandem Mass Spectrometry

Wang

Hartmann-Fischbach

Krueger

et al. 2015

AJAC

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A simple, rapid, sensitive and reproducible method for enantiomer analysis of methamphetamine, amphetamine, cathinone and methcathinone was developed and validated. The compounds were extracted from equine plasma and urine using a fast liquid-liquid extraction procedure. Only one milliliter plasma and one hundred microliter urine sample is needed for analysis. The extraction procedure had good recovery (>70%) and the matrix effect was negligible. Enantiomer differentiation and confirmation were achieved using liquid chromatography with chiral stationary phase and mass spectrometry detection. The method demonstrated excellent reproducibility with intraday and inter-day precision of lower than 5%. The lower limits of detection for all of the compounds studied here were at low pg/mL level for both plasma and urine. This is the first report of the analysis of four chiral compounds in equine plasma and urine. Routine application was demonstrated for (S)-and (R)-enantiomer differentiation.

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“…Column switching is often used in bioanalysis of chiral drugs, where separation of matrixrelated proteins using an achiral column is carried out prior to enantiopurity analysis using a chiral column. 6 Analysis of complex mixtures of closely related stereoisomers has long relied on the use of fixed tandem column arrangements, a typical approach being the pairing of an achiral column (to separate diastereomers) with a chiral column (to separate enantiomers). [7][8][9] A certain inconvenience is involved in the setup and use of tandem column arrangements, as mechanical plumbing of the assembly is required, and the two columns used in the study become unavailable for conventional single column use while in the tandem arrangement.…”

Section: Introductionmentioning

confidence: 99%

Solving multicomponent chiral separation challenges using a new SFC tandem column screening tool

et al. 2006

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A tool for improved tandem column chiral supercritical fluid chromatography (SFC) method development screening was prepared by modification of a commercial analytical SFC instrument with two different software-controllable, six position high-pressure column selection valves, each controlling a bank of five different columns and a pass through line. The resulting instrument, which has the ability to screen 10 different individual columns and 25 different tandem column arrangements, is a useful tool for facilitating the screening of tandem column SFC arrangements for separation of complex mixtures of stereoisomers or other multicomponent mixtures. Strategies for optimal use of the instrument are discussed, and several examples of the use of the instrument in developing tandem SFC methods for resolution of multicomponent mixtures are presented.

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Role of biological matrices during the analysis of chiral drugs by liquid chromatography

Cited by 42 publications

References 170 publications

Two‐dimensional reverse phase‐reverse phase chromatography: A simple and robust platform for sensitive quantitative analysis of peptides by LC/MS. Hardware design

Two‐dimensional reverse phase‐reverse phase chromatography: A simple and robust platform for sensitive quantitative analysis of peptides by LC/MS. Hardware design

Fast and Sensitive Chiral Analysis of Amphetamines and Cathinones in Equine Urine and Plasma Using Liquid Chromatography Tandem Mass Spectrometry

Solving multicomponent chiral separation challenges using a new SFC tandem column screening tool

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