Role of Biogenic Amines in Oviposition by the Diamondback Moth, Plutella xylostella L

Abstract

Oviposition is an important reproductive behavior that is triggered by mating in insects, and biogenic amines might be involved in its regulation. The effects of biogenic amines on oviposition have only been studied in a few insect species, and the findings to date have not been conclusive. In addition, there are few studies on the effects of biogenic amines on oviposition of the diamondback moth, Plutella xylostella L. Here, we tested how mating and biogenic amines regulate oviposition of P. xylostella by injecting amines and amine receptor antagonists into virgin and mated females and counting the number of eggs laid afterward. Biogenic amines of octopamine and tyramine could induce virgin adults of P. xylostella to lay eggs, while dopamine and serotonin had no such effect on oviposition. Furthermore, the octopamine antagonists mianserin, epinastine, and phentolamine inhibited oviposition by mated females. The tyramine antagonist yohimbine, dopamine antagonist SCH23390, and serotonin antagonist ketanserin did not block oviposition by mated females, and octopamine and tyramineinducing oviposition by virgin females could be inhibited by the octopamine antagonists mianserin and epinastine instead of the tyramine antagonist yohimbine. We conclude that octopamine and its receptors are involved in mating-triggered oviposition in P. xylostella, while tyramine acts as a subsidiary. Further, the inducing effect of tyramine on oviposition is achieved via octopamine receptors instead of tyramine receptors. This experiment is helpful to further understand the role of biogenic amines in mating regulation and to provide a new strategy for controlling P. xylostella.

A new pest management strategy: transforming a non‐host plant into a dead‐end trap crop for the diamondback moth Plutella xylostella L.

“…DBM females start to lay eggs soon after mating, and the oviposition period lasts for 4–5 days 24,31 . In the present study, the faba beans with the Chinese kale extract still had a significant attraction effect on DBM oviposition 15 days after spraying the extract (Fig.…”

The octopamine receptor, OA2B2, modulates stress resistance and reproduction in Nilaparvata lugens Stål (Hemiptera: Delphacidae)

“…Elevated OA and OA receptors were involved in oviposition in Plutella xylostella (Li et al, 2020). OA regulated reproductive functions in D. melanogaster by controlling ovulation, and low OA concentrations resulted in fewer laid eggs (Monastirioti, 2003), indicating OA and OA receptors modulate insect ovulation behaviour.…”

Assessment of Insecticidal Activity of Benzylisoquinoline Alkaloids from Chilean Rhamnaceae Plants against Fruit-Fly Drosophila melanogaster and the Lepidopteran Crop Pest Cydia pomonella

“…Different studies on these receptors indicate that they can be blocked by some known drugs. So far, phentolamine, mianserin, cyproheptadine, and promethazine have been reported as the most potent inhibitors of this receptor [61,62]. In D. melanogaster, the dopamine receptors (DOP1, DOP2, and DOP3) are paralog with Octβ3R and both can be inhibited by mianserine, epinastine, and spiperone, even though the inhibition of DOP receptors occurs at lower concentrations than those found for Octβ3R [41,63].…”