Role of Bacteriophages for Optimized Health and Production of Poultry

Abbas, Rao Zahid; Alsayeqh, Abdullah F.; Aqib, Amjad Islam

doi:10.3390/ani12233378

Cited by 6 publications

(4 citation statements)

References 76 publications

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“…It is worth noting that phage preparations are highly specific agents, often active only against a specific species or bacterial strain of the host [ 26 , 27 ]. In view of the above, if such agents are approved for use in the future, it will be important to develop a biopreparation method effective against autochthonous strains (serovars) of Salmonella .…”

Section: Introductionmentioning

confidence: 99%

Newly Isolated Virulent Salmophages for Biocontrol of Multidrug-Resistant Salmonella in Ready-to-Eat Plant-Based Food

Wójcicki

Świder

Średnicka

et al. 2023

IJMS

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Due to irrational antibiotic stewardship, an increase in the incidence of multidrug resistance of bacteria has been observed recently. Therefore, the search for new therapeutic methods for pathogen infection treatment seems to be necessary. One of the possibilities is the utilization of bacteriophages (phages)—the natural enemies of bacteria. Thus, this study is aimed at the genomic and functional characterization of two newly isolated phages targeting MDR Salmonella enterica strains and their efficacy in salmonellosis biocontrol in raw carrot–apple juice. The Salmonella phage vB_Sen-IAFB3829 (Salmonella phage strain KKP 3829) and Salmonella phage vB_Sen-IAFB3830 (Salmonella phage strain KKP 3830) were isolated against S. I (6,8:l,-:1,7) strain KKP 1762 and S. Typhimurium strain KKP 3080 host strains, respectively. Based on the transmission electron microscopy (TEM) and whole-genome sequencing (WGS) analyses, the viruses were identified as members of tailed bacteriophages from the Caudoviricetes class. Genome sequencing revealed that these phages have linear double-stranded DNA and sizes of 58,992 bp (vB_Sen-IAFB3829) and 50,514 bp (vB_Sen-IAFB3830). Phages retained their activity in a wide range of temperatures (from −20 °C to 60 °C) and active acidity values (pH from 3 to 11). The exposure of phages to UV radiation significantly decreased their activity in proportion to the exposure time. The application of phages to the food matrices significantly reduced the level of Salmonella contamination compared to the control. Genome analysis showed that both phages do not encode virulence or toxin genes and can be classified as virulent bacteriophages. Virulent characteristics and no possible pathogen factors make examined phages feasible to be potential candidates for food biocontrol.

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Section: Introductionmentioning

confidence: 99%

Newly Isolated Virulent Salmophages for Biocontrol of Multidrug-Resistant Salmonella in Ready-to-Eat Plant-Based Food

Wójcicki

Świder

Średnicka

et al. 2023

IJMS

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“…The question about possible interactions between bacteriophages and eukaryotic organisms appeared important as it has been recognized that these viruses are the most abundant biological entities on Earth, that they occur in large numbers in human/animal gut, and that they might be used to combat bacterial infections (the re-discovery of the primary findings made over a century ago) 2 – 12 . Although bacteriophages are obligatory parasites of bacteria 1 , recent studies provided evidence that they influence not only the microbiome of human/animal gut but also affect the eukaryotic host immune system 13 , 14 , 18 – 20 .…”

Section: Discussionmentioning

confidence: 99%

Bacteriophage DNA induces an interrupted immune response during phage therapy in a chicken model

Podlacha,

Gaffke,

Grabowski

et al. 2024

Nat Commun

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One of the hopes for overcoming the antibiotic resistance crisis is the use of bacteriophages to combat bacterial infections, the so-called phage therapy. This therapeutic approach is generally believed to be safe for humans and animals as phages should infect only prokaryotic cells. Nevertheless, recent studies suggested that bacteriophages might be recognized by eukaryotic cells, inducing specific cellular responses. Here we show that in chickens infected with Salmonella enterica and treated with a phage cocktail, bacteriophages are initially recognized by animal cells as viruses, however, the cGAS-STING pathway (one of two major pathways of the innate antiviral response) is blocked at the stage of the IRF3 transcription factor phosphorylation. This inhibition is due to the inability of RNA polymerase III to recognize phage DNA and to produce dsRNA molecules which are necessary to stimulate a large protein complex indispensable for IRF3 phosphorylation, indicating the mechanism of the antiviral response impairment.

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“…But in recent decades, the rise of antibiotic-resistant bacteria has prompted renewed interest in phage therapy of human and animal pathogens. Animal diseases studied for treatment include agriculturally important species such as poultry (Abbas et al, 2022 ) and cattle (Ngassam-Tchamba et al, 2020 ) but also aquaculture species (Kalatzis et al, 2018 ).…”

Section: Bacterial Pathogens Of Animalsmentioning

confidence: 99%