Role of Bacillus tequilensis EA-CB0015 cells and lipopeptides in the biological control of black Sigatoka disease

Cuellar‐Gaviria, Tatiana Z.; González-Jaramillo, Lina María; Villegas-Escobar, Valeska

doi:10.1016/j.biocontrol.2020.104523

Cited by 12 publications

(5 citation statements)

References 56 publications

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“…EA-CB0015 was originally identified as B. subtilis ( Ceballos et al, 2012 ) and later referred to as B. tequilensis ( Cuellar-Gaviria et al, 2021 ). This ambiguity derived from the use of 16S rRNA gene phylogenies, which do not provide sufficient resolution at the species level for strains within the B. subtilis complex ( Rooney et al, 2009 ).…”

Section: Resultsmentioning

confidence: 99%

“…Mitigation of black Sigatoka represents a significant burden to Colombian producers, with a cost $65 million per year due to weekly application of fungicides (S. Zapata, personal communication). EA-CB0015 suppresses black Sigatoka disease through the colonization of banana leaves and the production of antifungal lipopeptides (iturins, plipastatins, and surfactins; Villegas-Escobar et al, 2013 ; Mosquera et al, 2014 ; Cuellar-Gaviria et al, 2021 ). In addition to bananas, the strain also reduced the severity of anthracnose ( Colletotrichum spp.)…”

Section: Introductionmentioning

confidence: 99%

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The genome of Bacillus tequilensis EA-CB0015 sheds light into its epiphytic lifestyle and potential as a biocontrol agent

2023

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Different Bacillus species have successfully been used as biopesticides against a broad range of plant pathogens. Among these, Bacillus tequilensis EA-CB0015 has shown to efficiently control Black sigatoka disease in banana plants, presumably by mechanisms of adaptation that involve modifying the phyllosphere environment. Here, we report the complete genome of strain EA-CB0015, its precise taxonomic identity, and determined key genetic features that may contribute to its effective biocontrol of plant pathogens. We found that B. tequilensis EA-CB0015 harbors a singular 4 Mb circular chromosome, with 3,951 protein-coding sequences. Multi-locus sequence analysis (MLSA) and average nucleotide identity (ANI) analysis classified strain EA-CB0015 as B. tequilensis. Encoded within its genome are biosynthetic gene clusters (BGCs) for surfactin, iturin, plipastatin, bacillibactin, bacilysin, subtilosin A, sporulation killing factor, and other natural products that may facilitate inter-microbial warfare. Genes for indole-acetic acid (IAA) synthesis, the use of diverse carbon sources, and a multicellular lifestyle involving motility, biofilm formation, quorum sensing, competence, and sporulation suggest EA-CB0015 is adept at colonizing plant surfaces. Defensive mechanisms to survive invading viral infections and preserve genome integrity include putative type I and type II restriction modification (RM) and toxin/antitoxin (TA) systems. The presence of bacteriophage sequences, genomic islands, transposable elements, virulence factors, and antibiotic resistance genes indicate prior occurrences of genetic exchange. Altogether, the genome of EA-CB0015 supports its function as a biocontrol agent against phytopathogens and suggest it has adapted to thrive within phyllosphere environments.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The genome of Bacillus tequilensis EA-CB0015 sheds light into its epiphytic lifestyle and potential as a biocontrol agent

2023

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“…Inoculating B. subtilis SF1 after infection led to a significant decrease in the FW incidence rate (45.56%) , and healthy potato seedlings treated with B. subtilis SF1 appeared to grow vigorously, compared to untreated potato seedlings. Surfactin is essential for biofilm formation and surface motility of plant growth-promoting organisms [ 74 , 75 ]. Siderophore production and phosphate solubilization, both important for plant growth, were detected in B. subtilis SF1.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of Surfactin from Bacillus subtilis SF1 against Fusarium foetens: A Novel Pathogen Inducing Potato Wilt

Liu

Jin

et al. 2023

JoF

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Fusarium wilt is a severe and worldwide disease in potato cultivation. In this study, Fusarium foetens was first identified as the pathogen of potato wilt. Bacillus subtilis SF1 has the potential for controlling potato wilt induced by F. foetens, resulting in a mycelium growth inhibition of 52.50 ± 2.59% in vitro and a significant decrease in incidence rate by 45.56% in vivo. This research highlighted the antifungal activity of surfactin from B. subtilis SF1 and attempted to reveal the unknown antifungal mechanisms. Surfactin inhibited F. foetens mycelium growth beyond the concentration of 20 μg/μL. Surfactin-treated mycelium appeared to have morphological malformation. Surfactin enhanced reduced glutathione production and caused the increase in values of the extracellular fluids in OD260 and OD280. Surfactin induced differential protein expression and changed the genes’ transcription levels. Surfactin binds to fungal DNA via groove-binding mode, with a binding constant of Kb 2.97 × 104 M−1. Moreover, B. subtilis SF1 harbored genes encoding plant-promoting determinants, making potato seedlings grow vigorously. The results will help provide a comprehensive understanding of the mechanisms of surfactin against filamentous fungi and the application of surfactin-producing microbial in the biocontrol of plant pathogenic fungi.

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“…Bacillus tequilensis EA-CB0015 can produce lipopeptides to inhibit mycelial growth and ascospore germination of Pseudocercospora fijiensis in vitro, and colonize on the plant leaves' surface to alleviate the severity of black Sigatoka disease. 24 Bacillus tequilensis strain JN-369, isolated from xiangzaoxian no. 24 healthy rice plants, can promote seed germination and seedling growth, increase plant defense enzyme activities, reduce the severity of rice leaf blast and panicle blast, and increase rice yield.…”

Section: ■ Discussionmentioning

confidence: 99%

“…tequilensis and found that it has high biocontrol potential and antimicrobial activities against many pathogens. Bacillus tequilensis EA-CB0015 can produce lipopeptides to inhibit mycelial growth and ascospore germination of Pseudocercospora fijiensis in vitro , and colonize on the plant leaves’ surface to alleviate the severity of black Sigatoka disease Bacillus tequilensis strain JN-369, isolated from xiangzaoxian no.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Effects of Volatiles Produced by Bacillus tequilensis XK29 against the Black Spot Disease Caused by Ceratocystis fimbriata in Postharvest Sweet Potato

Guo

et al. 2021

J. Agric. Food Chem.

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Black spot disease caused by Ceratocystis fimbriata is destructive to the production, transportation, and storage of sweet potato. The antifungal effects of Bacillus tequilensis XK29 against C. fimbriata through volatile organic compounds (VOCs) were evaluated in this study. The activated carbon assay proved that XK29 could exert antibiotic effects through volatiles. By optimizing the wheat seed weight, inoculation method, concentration, volume, and time, the antifungal activity of XK29 was significantly improved. XK29 fumigation inhibited spore formation and germination and changed the cell morphology of C. fimbriata. During the storage of sweet potato tuber roots, XK29 effectively controlled black spot disease and reduced the weight loss and malondialdehyde content. Metabolomic analysis revealed that 21 volatile compounds were released from XK29. Isovaleric acid, isobutyric acid, and 2-methylbutanoic acid effectively inhibited the growth of C. fimbriata. These results indicate that B. tequilensis XK29 has a good potential to be developed as a microbial fumigation agent.

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Role of Bacillus tequilensis EA-CB0015 cells and lipopeptides in the biological control of black Sigatoka disease

Cited by 12 publications

References 56 publications

The genome of Bacillus tequilensis EA-CB0015 sheds light into its epiphytic lifestyle and potential as a biocontrol agent

The genome of Bacillus tequilensis EA-CB0015 sheds light into its epiphytic lifestyle and potential as a biocontrol agent

Mechanisms of Surfactin from Bacillus subtilis SF1 against Fusarium foetens: A Novel Pathogen Inducing Potato Wilt

Antibiotic Effects of Volatiles Produced by Bacillus tequilensis XK29 against the Black Spot Disease Caused by Ceratocystis fimbriata in Postharvest Sweet Potato

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