Antibiotic Effects of Volatiles Produced by <i>Bacillus tequilensis</i> XK29 against the Black Spot Disease Caused by <i>Ceratocystis fimbriata</i> in Postharvest Sweet Potato

Xu, Mingyu; Guo, Jianheng; Li, Tengjie; Zhang, Chunmei; Peng, Xue; Xing, Ke; Qin, Sheng

doi:10.1021/acs.jafc.1c04585

Cited by 28 publications

(8 citation statements)

References 47 publications

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“…Compared with other crops, sweetpotatoes can support more people per unit of land, and they are the staple food for large populations over the world [5]. However, sweetpotatoes are prone to diseases during postharvest storage and transportation, such as black rot [6], soft rot [7], and root rot [8]. Among them, black rot is the most typical.…”

Section: Introductionmentioning

confidence: 99%

Early Discrimination and Prediction of C. fimbriata-Infected Sweetpotatoes during the Asymptomatic Period Using Electronic Nose

Pang

Zhang

et al. 2022

Foods

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Sweetpotato is prone to disease caused by C. fimbriata without obvious lesions on the surface in the early period of infection. Therefore, it is necessary to explore the possibility of developing an efficient early disease detection method for sweetpotatoes that can be used before symptoms are observed. In this study, sweetpotatoes were inoculated with C. fimbriata and stored for different lengths of time. The total colony count was detected every 8 h; HS-SPME/GC–MS and E-nose were used simultaneously to detect volatile compounds. The results indicated that the growth of C. fimbriata entered the exponential phase at 48 h, resulting in significant differences in concentrations of volatile compounds in infected sweetpotatoes at different times, especially toxic ipomeamarone in ketones. The contents of volatile compounds were related to the responses of the sensors. E-nose was combined with multiple chemometrics methods to discriminate and predict infected sweetpotatoes at 0 h, 48 h, 64 h, and 72 h. Among the methods used, linear discriminant analysis (LDA) had the best discriminant effect, with sensitivity, specificity, precision, and accuracy scores of 100%. E-nose combined with K-nearest neighbours (KNN) achieved the best predictions for ipomeamarone contents and total colony counts. This study illustrates that E-nose is a feasible and promising technology for the early detection of C. fimbriata infection in sweetpotatoes during the asymptomatic period.

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Section: Introductionmentioning

confidence: 99%

Early Discrimination and Prediction of C. fimbriata-Infected Sweetpotatoes during the Asymptomatic Period Using Electronic Nose

Pang

Zhang

et al. 2022

Foods

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“…Previous studies found that B. tequilensis can quickly and stably colonize plants, and has a high propagation rate and a strong proliferative capacity [ 30 ]. B. tequilensis has high efficiency and broad-spectrum resistance to plant pathogens, has a combined treatment effect on plant diseases such as anthracnose [ 31 ] and black spot disease [ 32 ], has a high prevention effect, and has good application prospects for the prevention and control of plant diseases [ 33 ]. However, it has never been reported for γ-PGA production; therefore, B. tequilensis BL01 was selected for subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid

Wang

Zhou

et al. 2022

Microb Cell Fact

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Background Poly γ-glutamic acid (γ-PGA) is a promising biopolymer for various applications. For glutamic acid-independent strains, the titer of γ-PGA is too low to meet the industrial demand. In this study, we isolated a novel γ-PGA-producing strain, Bacillus tequilensis BL01, and multiple genetic engineering strategies were implemented to improve γ-PGA production. Results First, the one-factor-at-a-time method was used to investigate the influence of carbon and nitrogen sources and temperature on γ-PGA production. The optimal sources of carbon and nitrogen were sucrose and (NH4)2SO4 at 37 °C, respectively. Second, the sucA, gudB, pgdS, and ggt genes were knocked out simultaneously, which increased the titer of γ-PGA by 1.75 times. Then, the titer of γ-PGA increased to 18.0 ± 0.3 g/L by co-overexpression of the citZ and pyk genes in the mutant strain. Furthermore, the γ-PGA titer reached 25.3 ± 0.8 g/L with a productivity of 0.84 g/L/h and a yield of 1.50 g of γ-PGA/g of citric acid in fed-batch fermentation. It should be noted that this study enables the synthesis of low (1.84 × 105 Da) and high (2.06 × 106 Da) molecular weight of γ-PGA by BL01 and the engineering strain. Conclusion The application of recently published strategies to successfully improve γ-PGA production for the new strain B. tequilensis BL01 is reported. The titer of γ-PGA increased 2.17-fold and 1.32-fold compared with that of the wild type strain in the flask and 5 L fermenter. The strain shows excellent promise as a γ-PGA producer compared with previous studies. Meanwhile, different molecular weights of γ-PGA were obtained, enhancing the scope of application in industry.

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“…Previous studies found that B. tequilensis can quickly and stably colonize plants, and has a high propagation rate and strong in proliferative capacity (Shultana et al 2021). B. tequilensis has high e ciency and broad-spectrum plant pathogen resistance, has a combined treatment effect on plant diseases such as anthracnose (Kwon et al 2022) and black spot disease (Xu et al 2021), has a high prevention effect, and has good application prospects for the prevention and control of plant diseases (Guerrero-Barajas et al 2020). However, it has never been reported for γ-PGA production; therefore, B. tequilensis BL01 was selected for subsequent experiments.…”

Section: Screening Of Highly γ-Pga-producing Strainsmentioning

confidence: 99%

Enhanced poly-γ-glutamic acid production by a newly isolated Bacillus tequilensis BL01

Wang

Zhou

Gao

et al. 2022

Preprint

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Poly γ-glutamic acid (γ-PGA) is a promising biopolymer for various applications. In this study, we isolated a novel γ-PGA-producing strain, Bacillus tequilensis BL01. The one-factor-at-a-time method was used to investigate the influence of carbon and nitrogen sources and temperature on γ-PGA production. The optimal carbon and nitrogen sources were sucrose and (NH4)2SO4, respectively. The optimal temperature for γ-PGA production was determined to be 37°C. Response surface methodology was used to determine the optimum medium components: 68.9 g/L sucrose, 47.7 g/L monosodium glutamate, and 2.5 g/L K2HPO4. γ-PGA titer increased significantly from 8.6 g/L to 21.1 g/L when strain BL01 was cultivated in the optimized medium. Furthermore, γ-PGA titer reached 54.7 g/L with a productivity of 1.37 g/L/h and a yield of 2.47 g of γ-PGA/g of L-glutamic acid with the optimized medium in fed-batch fermentation. It should be noted that the γ-PGA yield in this study was the highest of all reported studies, indicating a great potential for the industrial production of γ-PGA.

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Antibiotic Effects of Volatiles Produced by Bacillus tequilensis XK29 against the Black Spot Disease Caused by Ceratocystis fimbriata in Postharvest Sweet Potato

Cited by 28 publications

References 47 publications

Early Discrimination and Prediction of C. fimbriata-Infected Sweetpotatoes during the Asymptomatic Period Using Electronic Nose

Early Discrimination and Prediction of C. fimbriata-Infected Sweetpotatoes during the Asymptomatic Period Using Electronic Nose

Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid

Enhanced poly-γ-glutamic acid production by a newly isolated Bacillus tequilensis BL01

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