Role of Auxin and Polyamines in Adventitious Root Formation in Relation to Changes in Compounds Involved in Rooting

Nag, Sudipa; Saha, Karnica; Choudhuri, M. A.

doi:10.1007/s003440010016

Cited by 133 publications

(114 citation statements)

References 27 publications

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“…Similarly, Druart [1997], using IBA (1 mg l -1 ) combined with IAA (0.1 mg l -1 ) obtained 100% rooting of the cultivar 'Compact Spartan'. The successful rooting could be due to the specific effect of IBA which was found to increase the level of endogenous free IAA [Nag et al 2001]. Besides, it is demonstrated that rhizogenesis is induced only by free auxins [De Klerk et al 1999, Nag et al 2001.…”

Section: Discussionmentioning

confidence: 95%

“…The successful rooting could be due to the specific effect of IBA which was found to increase the level of endogenous free IAA [Nag et al 2001]. Besides, it is demonstrated that rhizogenesis is induced only by free auxins [De Klerk et al 1999, Nag et al 2001. Moreover, IBA is considered IAA precursor since in several plants auxin activity of IBA is completely dependent on its conversion to IAA by peroxisomal enzymes [Strader and Bartel 2011].…”

Section: Discussionmentioning

confidence: 99%

“…It was well documented that auxins are only needed during the induction of root formation [De Klerk et al 1999, Nag et al 2001.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, these authors concluded that free Put can be considered as a marker of in vitro root induction phase. Nag et al [2001] suggested that treatment with IBA reduces IAA-oxidase activity, and thus increased endogenous IAA level at very early rhizogenesis induction phase. Both IBA and Put enhanced at later phase peroxidase activity which is involved in cell wall formation, related to root initiation.…”

Section: Discussionmentioning

confidence: 99%

“…Adventitious root formation in vitro has been studied extensively in many species, e.g. in mung bean [Nag et al 2001] and apple [De Klerk et al 1999]. Generally, adventitious root formation both in vitro and in vivo can be divided into four phases and in apple the process is as follows: 1) induction (dedifferentiation phase, lasting approximately 24 h, some cells close to the vascular tissue turn meristematic and sensitive to auxin-inducing rhizogenesis; 2) initiation (next 2-4 days) when competent cells Fig.…”

Section: Introductionmentioning

confidence: 99%

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ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Podwyszyńska

Cieślińska

2018

Acta Sci.Pol.Hortorum Cultus

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For breeding purposes, the number of neo-tetraploids of apple cultivars have been derived by in vitro technique. The first attempts at rooting and acclimatization of tetraploid shoots failed. The aim of the study was to develop an effective method for rooting microcuttings of apple neo-tetraploids. In the first stage of the study, in vitro rooting method was optimized for the shoots of diploid donor cultivars. Shoots were rooted on Murashige and Skoog [1962] (MS) medium with a reduced content of nitrogen, in the presence of auxins alone or in combination (indole-3-butyric acid -IBA, 1-naphthaleneacetic acid -NAA and indole--3-acetic acid -IAA) with addition of putrescine, arginine or ornithine. The compounds were applied continuously for 25 days (one-step rooting system) or for seven days with subsequent transplanting shoots onto a medium without these compounds (two-step rooting method). Tetraploid microcuttings of the cultivars 'Free Redstar', 'Gala Must', 'Pinova' and 'Redchief' were evaluated for rooting on the selected medium considered optimal for their diploid counterparts. The shoots of all diploid apple scion cultivars had low rooting capacity. IBA alone poorly stimulated root formation. Significant improvement of rooting to 60-80% was achieved through the application of auxins, 2.5 µM IBA or 1.3 µM NAA combined with 5 µM IAA and 50 µM putrescine in the two-step rooting system with darkness and increased temperature of 26°C during seven-day induction phase. The replacement of benzyladenine (BA) by meta-Topolin (m-T) in the last multiplication subculture influenced positively shoot acclimatization. Tetraploids had comparable or slightly lower rooting and acclimatization ability compared to their diploid counterparts.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

“…It was well documented that auxins are only needed during the induction of root formation [De Klerk et al 1999, Nag et al 2001.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Podwyszyńska

Cieślińska

2018

Acta Sci.Pol.Hortorum Cultus

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Adventitious Root Formation: New Insights and Perspectives

Geiss

Gutierrez

Bellini

2018

Annual Plant Reviews Online

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The root system of a plant consists of the primary, lateral and adventitious roots. Lateral roots always develop from roots whereas adventitious roots form from stem or leaf‐derived cells. Adventitious rooting is an essential step in the vegetative propagation of economically important horticultural and woody species. It allows clonal propagation and rapid fixation of superior genotypes prior to their introduction into production or breeding programs. Problems associated with rooting of cuttings frequently result in significant economic losses. Development of adventitious roots is a complex process that is affected by multiple factors including phytohormones, light, nutritional status, associated stress responses such as wounding, and genetic characteristics. How endogenous and environmental factors interact to control adventitious root formation is still poorly understood, although significant progress has been made in the understanding of the molecular control of root and lateral root development. In this review, we will summarize the current knowledge on the physiological aspects of AR formation and highlight the recent progress made in the identification of putative molecular players involved in the control of adventitious rooting.

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Adventitious Root Formation: New Insights and Perspectives

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Role of Auxin and Polyamines in Adventitious Root Formation in Relation to Changes in Compounds Involved in Rooting

Cited by 133 publications

References 27 publications

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

ROOTING SHOOTS OF APPLE VARIETIES AND THEIR TETRAPLOIDS OBTAINED BY THE in vitro TECHNIQUE

Adventitious Root Formation: New Insights and Perspectives

Adventitious Root Formation: New Insights and Perspectives

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