Adventitious Root Formation: New Insights and Perspectives

Geiss, Gaia; Gutierrez, Laurent; Bellini, Catherine

doi:10.1002/9781444310023.ch5

Cited by 95 publications

(97 citation statements)

References 135 publications

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“…The biological processes involved in AR formation are complex, and the temporal phases can be described as induction, initiation, activation of root primordium and out-growth (Legu e et al, 2014). These processes are influenced by multiple factors, such as the genetic background and the physiological status of the mother plants, the application of hormones and environmental conditions (Geiss et al, 2010;Pacurar et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

The auxin receptor TIR1 homolog (PagFBL 1) regulates adventitious rooting through interactions with Aux/IAA28 in Populus

Shu

Zhou

Jiang

et al. 2018

Plant Biotechnology Journal

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Adventitious roots occur naturally in many species and can also be induced from explants of some tree species including Populus, providing an important means of clonal propagation. Auxin has been identified as playing a crucial role in adventitious root formation, but the associated molecular regulatory mechanisms need to be elucidated. In this study, we examined the role of PagFBL1, the hybrid poplar (Populus alba × P. glandulosa clone 84K) homolog of Arabidopsis auxin receptor TIR1, in adventitious root formation in poplar. Similar to the distribution pattern of auxin during initiation of adventitious roots, PagFBL1 expression was concentrated in the cambium and secondary phloem in stems during adventitious root induction and initiation phases, but decreased in emerging adventitious root primordia. Overexpressing PagFBL1 stimulated adventitious root formation and increased root biomass, while knock-down of PagFBL1 transcript levels delayed adventitious root formation and decreased root biomass. Transcriptome analyses of PagFBL1 overexpressing lines indicated that an extensive remodelling of gene expression was stimulated by auxin signalling pathway during early adventitious root formation. In addition, PagIAA28 was identified as downstream targets of PagFBL1. We propose that the PagFBL1-PagIAA28 module promotes adventitious rooting and could be targeted to improve Populus propagation by cuttings.

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Section: Introductionmentioning

confidence: 99%

The auxin receptor TIR1 homolog (PagFBL 1) regulates adventitious rooting through interactions with Aux/IAA28 in Populus

Shu

Zhou

Jiang

et al. 2018

Plant Biotechnology Journal

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“…The technique exploits the ability of non-meristematic tissues, which do not normally form roots (e.g. stem and leaves), to dedifferentiate and develop adventitious roots (ARs) (Geiss et al, 2009). AR formation is controlled by several environmental and endogenous factors, including temperature, light (intensity and wavelength), carbohydrate availability, phenolic compounds and phytohormones (Eliasson, 1978;Kevers et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Several genes and phytohormones have been implicated in the control of AR formation, and the action of auxin [indole-3-acetic acid (IAA)], jasmonate (JA), strigolactones (SLs) and cytokinins has been studied in the most detail (Sorin et al, 2005;Ram ırez-Carvajal et al, 2009;Gutierrez et al, 2012;Rasmussen et al, 2012). Exogenous IAA applications have been used for years to synchronize the rooting of in vitro cuttings and increase root production in poorly rooting phenotypes (Geiss et al, 2009). Evidence of IAA action has also been obtained from analyses of Arabidopsis mutants overproducing IAA, such as sur1 and sur2, which produce abundant ARs (Boerjan et al, 1995;Delarue et al, 1998;Barlier et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Gibberellins inhibit adventitious rooting in hybrid aspen and Arabidopsis by affecting auxin transport

et al. 2014

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SUMMARYKnowledge of processes involved in adventitious rooting is important to improve both fundamental understanding of plant physiology and the propagation of numerous plants. Hybrid aspen (Populus tremula 3 tremulo€ ıdes) plants overexpressing a key gibberellin (GA) biosynthesis gene (AtGA20ox1) grow rapidly but have poor rooting efficiency, which restricts their clonal propagation. Therefore, we investigated the molecular basis of adventitious rooting in Populus and the model plant Arabidopsis. The production of adventitious roots (ARs) in tree cuttings is initiated from the basal stem region, and involves the interplay of several endogenous and exogenous factors. The roles of several hormones in this process have been characterized, but the effects of GAs have not been fully investigated. Here, we show that a GA treatment negatively affects the numbers of ARs produced by wild-type hybrid aspen cuttings. Furthermore, both hybrid aspen plants and intact Arabidopsis seedlings overexpressing AtGA20ox1, PttGID1.1 or PttGID1.3 genes (with a 35S promoter) produce few ARs, although ARs develop from the basal stem region of hybrid aspen and the hypocotyl of Arabidopsis. In Arabidopsis, auxin and strigolactones are known to affect AR formation. Our data show that the inhibitory effect of GA treatment on adventitious rooting is not mediated by perturbation of the auxin signalling pathway, or of the strigolactone biosynthetic and signalling pathways. Instead, GAs appear to act by perturbing polar auxin transport, in particular auxin efflux in hybrid aspen, and both efflux and influx in Arabidopsis.

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“…To decipher the role of auxin in the control of adventitious root (AR) development, which is a complex trait with high phenotypic plasticity (27,28), we previously identified a regulatory module composed of three ARF genes (two activators AFR6 and ARF8, and one repressor ARF17) and their regulatory microRNAs (miR167 and miR160) (29). These genes display overlapping expression domains, interact genetically and regulate each other's expression at transcriptional and post-transcriptional levels by modulating the availability of their regulatory microRNAs miR160 and miR167 (29).…”

Section: Introductionmentioning

confidence: 99%

Molecular framework for TIR1/AFB-Aux/IAA-dependent auxin sensing controlling adventitious rooting in Arabidopsis

Lakehal

Chaabouni

Cavel

et al. 2019

Preprint

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In Arabidopsis thaliana, canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR (ARF) family, most of which interact with 29 auxin/indole acetic acid (Aux/IAA) repressors, themselves forming, in the presence of auxin, coreceptor complexes with one of six TRANSPORT INHIBITOR1/AUXIN-SIGNALLING F-BOX PROTEINS (TIR1/AFB). Different combinations of co-receptors drive specific sensing outputs, allowing auxin to control a myriad of processes. Considerable efforts have been made to discern the temporal and spatial specificity of auxin action. However, owing to a lack of obvious phenotype in single loss-of-function mutants in Aux/IAA genes, most genetic studies have relied on gain-of-function mutants, which are highly pleiotropic. In this article, we describe a molecular framework for the role of several members of the auxin sensing machinery.Using loss-of-function mutants, we demonstrate that TIR1 and AFB2 are positive regulators, whereas IAA6, IAA9 and IAA17 are negative regulators of adventitious root (AR) formation. The three Aux/IAA proteins interact with ARF6 and/or ARF8, which we have previously shown to be positive regulators of AR formation upstream of jasmonate, and likely repress their activity. Our data also suggest a dual role for TIR1 in the control of JA biosynthesis and conjugation, as revealed by upregulation of several JA biosynthesis genes in the tir1-1 mutant. In conclusion, we propose that in the presence of auxin, TIR1 and AFB2 form specific sensing complexes with IAA6, IAA9 and/or IAA17 that modulate JA homeostasis to control AR initiation.

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Adventitious Root Formation: New Insights and Perspectives

Cited by 95 publications

References 135 publications

The auxin receptor TIR1 homolog (PagFBL 1) regulates adventitious rooting through interactions with Aux/IAA28 in Populus

The auxin receptor TIR1 homolog (PagFBL 1) regulates adventitious rooting through interactions with Aux/IAA28 in Populus

Gibberellins inhibit adventitious rooting in hybrid aspen and Arabidopsis by affecting auxin transport

Molecular framework for TIR1/AFB-Aux/IAA-dependent auxin sensing controlling adventitious rooting in Arabidopsis

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