Role of angiogenesis in oral squamous cell carcinoma development and metastasis: an immunohistochemical study

Shivamallappa, Shivakumar Madappa; Venkatraman, Narayan; Shreedhar, Balasundari; Mohanty, Leeky; Shenoy, Sadhana

doi:10.4248/ijos11077

Cited by 33 publications

(26 citation statements)

References 40 publications

(46 reference statements)

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“…The purpose of angiogenesis in tumours is to sequester nutrients from the host for tumour cell survival and tumour growth (Carmeliet and Jain, 2000). Extensive studies pointed out that angiogenesis have been associated with tumour progression and aggressiveness in a number of malignancies, including oral cancer (Uehara et al, 2004;Shivamallappa et al, 2011). In the present study, we noticed over expression of VEGF in the buccal mucosa of hamsters treated with DMBA alone.…”

Section: Discussionsupporting

confidence: 61%

Proapoptotic, Anti-Cell Proliferative, Anti-Inflammatory And Antiangiogenic Potential Of Carnosic Acid During 7,12 Dimethylbenz[A]Anthracene-Induced Hamster Buccal Pouch Carcinogenesis

Rajasekaran¹,

Manoharan²,

Silvan³

et al. 2012

Afr. J. Trad. Compl. Alt. Med.

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The present study has investigated the modulating effect of carnosic acid on the expression pattern of cell proliferative (proliferating cell nuclear antigen (PCNA) cyclin D1 and a transcription factor c-fos), apoptotic (p53, Bcl-2, Bax caspase -3 and 9), inflammatory (Nuclear factor kappa B (NFκB) and cyclooxygenase-2 (COX-2) and angiogenic (vascular endothelial growth factor (VEGF) markers during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Oral tumors were developed in the hamsters buccal pouches by painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. Hundred per cent tumour formation (well-differentiated squamous cell carcinoma) accompanied by deregulation in the above mentioned molecular markers was noticed in hamsters treated with DMBA alone (tumour bearing hamsters). Oral administration of carnosic acid at dose of 10mg/kg bw to hamsters treated with DMBA not only completely prevented the tumour formation, but also corrected the abnormalities in the expression pattern of molecular markers. The present study suggests that carnosic acid might have inhibited the tumour formation by exerting anti-cell-proliferative, anti-inflammatory, anti-angiogenic and apoptotic potential during DMBA-induced hamster buccal pouch carcinogenesis.

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Section: Discussionsupporting

confidence: 61%

Proapoptotic, Anti-Cell Proliferative, Anti-Inflammatory And Antiangiogenic Potential Of Carnosic Acid During 7,12 Dimethylbenz[A]Anthracene-Induced Hamster Buccal Pouch Carcinogenesis

Rajasekaran¹,

Manoharan²,

Silvan³

et al. 2012

Afr. J. Trad. Compl. Alt. Med.

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“…Members of the human epidermal receptor (HER) family including the EGFR and to a lesser extent HER2 and HER3 play key roles in driving the oncogenic pathways that are important for cancer cell growth and proliferation, survival, and angiogenesis. Increased expression of the epidermal growth factor receptor (EGFR) can play a critical role in oral tumor development and progression (Grandis and Tweardy 1993;Shivamallappa et al 2011), and EGFR increases progressively from oral premalignant lesions to invasive OSCC (Shin et al 1994). MMPs are a family of secreted proteins that remodel and digest the extracellular matrix.…”

Section: Discussionmentioning

confidence: 99%

Receptor for activated C kinase 1 (RACK1): a regulator for migration and invasion in oral squamous cell carcinoma cells

Guo

Feng

et al. 2011

J Cancer Res Clin Oncol

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Purpose Receptor of activated protein kinase C 1 (RACK1) has been identified as an anchoring or adaptor protein in multiple intracellular signal transduction pathways. Our previous study has showed that the expression of RACK1 was paralleled with proliferation and correlated with metastasis and clinical outcome. However, the underlined mechanism has not been uncovered. Materials and methods We first selected a most effective siRNA among three siRNAs (siRNA-1, siRNA-2 and siRNA-3) targeting different regions in the RACK1 mRNA and re-evaluated the anticancer effect of RACK1 silencing on HSC-3 and Cal-27 cell lines by cell growth inhibition. And then, we investigated whether knockdown of RACK1 could inhibit cell adhesion, migration and invasion in these two cell lines. To further understand the molecular mechanism of RACK1 in these processes, the expressions of EGFR, pEGFR, HER2, MMP-2 and MMP-9 were detected by western blot. ResultsWe verified that the silence of RACK1 gene in two OSCC cell lines could not only inhibit cell proliferation but also decrease the invasion, migration and adhesion capability of the tumor cells. Further, western blot analysis deduced that it might be related to the decrease in protein expression of EGFR, pEGFR, HER2, MMP-2 and MMP-9. Conclusion Our results clearly showed the significance of RACK1-induced OSCC cell migration, invasion and adhesion, which could explain the underlined mechanism of the effect of the gene on metastasis and clinical outcome. Also, our results confirmed its role to be a prognostic indicator and a promising drug target for OSCC cell metastasis.

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“…One study conducted on head and neck malignant tumors presented correlations between vessel density, bad prognosis and onset of metastases [33,34], while another study found no correlation between angiogenesis and tumor stage and prognosis [35]. It is still unclear whether these discrepancies due to different tumor stages, localization or methods of analysis [36].…”

Section: Discussionmentioning

confidence: 99%

Correlation Between Microvessel Density and Morphological Features in Skin Squamous Cell Carcinoma

Kakasheva-Mazhenkovska

Basheska

Crvenkova

et al. 2017

Prilozi

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Introduction. Abnormal angiogenesis is described in tumor growth and it facilitates its metastatic spread. Tumors with high angiogenic activity belong to the category of aggressive tumors with poor prognosis for patients. The aim of this study was to determine the blood vessels density (BVD), i.e. neovascularization at the tumor invasive front in skin squamous cell carcinoma (SCC) in order to determine its possible role in the tumor progression, and to correlate it to the blood vessels density of healthy skin and with the prognostic parameters of the TNM classification: T status, depth of tumor invasion (DI) and tumor histological grade (G), which were also correlated between each other. Material and Methods. The material consisted of surgical specimens obtained from 30 patients with skin SCC, who underwent surgery. Tissue samples were routinely processed by standard paraffin technique stained by Hematoxilin-Eosin and immunohistochemically with antibodies against smooth muscle actin (SMA) and CD34. The BVD in the invasive front of the neoplasms was correlated to the healthy skin, tumor status (pT), depth of invasion and grade of histological differentiation (pG). Results. The histological analysis has shown a high statistical difference in the density of blood vessels in SCC compared to the healthy skin and statistical difference in BVD in neoplasms with different depth of invasion and different grade of differentiation. The density of neovascularzation increased with the deeper invasion and the worse differentiation. Conclusion. The increased vascularization at the invasive front of SCC with deeper invasion and worse differentiation has pointed out to its possible role in neoplasm progression.

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Role of angiogenesis in oral squamous cell carcinoma development and metastasis: an immunohistochemical study

Cited by 33 publications

References 40 publications

Proapoptotic, Anti-Cell Proliferative, Anti-Inflammatory And Antiangiogenic Potential Of Carnosic Acid During 7,12 Dimethylbenz[A]Anthracene-Induced Hamster Buccal Pouch Carcinogenesis

Proapoptotic, Anti-Cell Proliferative, Anti-Inflammatory And Antiangiogenic Potential Of Carnosic Acid During 7,12 Dimethylbenz[A]Anthracene-Induced Hamster Buccal Pouch Carcinogenesis

Receptor for activated C kinase 1 (RACK1): a regulator for migration and invasion in oral squamous cell carcinoma cells

Correlation Between Microvessel Density and Morphological Features in Skin Squamous Cell Carcinoma

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