Role of amino acids as additives on sperm motility, plasma membrane integrity and lipid peroxidation levels at pre-freeze and post-thawed ram semen

Sangeeta, Sharon; Arunachalam, A.; Kulkarni, Shrikant; Selvaraju, Sellappan

doi:10.1016/j.anireprosci.2015.08.008

Cited by 53 publications

(37 citation statements)

References 24 publications

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“…In line with our findings, it has been shown that the regular moderate-intensity exercise training improves in almost all sperm parameters in men (Sharma, Biedenharn, Fedor, & Agarwal, 2013). Indeed, the pathologic levels of ROS (even in testicular tissue further to seminal plasma) are able to adversely affect the sperm motility by initiating intensive lipid peroxidation of high polyunsaturated fatty acids (PUFA) within the sperm membrane (Sangeeta, Arangasamy, Kulkarni, & Selvaraju, 2015). Moreover, free radicals are able to potentially across the cell membrane and inhibit the glucose-6-phosphate dehydrogenase (G6PD) activity, which in turn ultimately ends with a diminished availability of intracellular nicotinamide adenine dinucleotide phosphate (NADPH), as well as pathologic accumulation of oxidised glutathione (Zhang et al, 2010).…”

Section: However This Situation Was Reversed In Dm + Ins and Dm + Insupporting

confidence: 88%

Moderate‐intensity exercise training ameliorates the diabetes‐suppressed spermatogenesis and improves sperm parameters: Insole and simultaneous with insulin

et al. 2019

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The current study was conducted to investigate the ameliorative effect of moderate‐intensity exercise training insole and simultaneous with insulin on diabetes (DM)‐induced pathogenesis at the testicular tissue and sperm level. For this purpose, 36 mature male Wistar rats were divided into six groups, including sedentary control (Con), exercise training (EX), sedentary experimental DM‐induced (SDM), exercise training + DM‐induced (DM + EX), insulin‐treated sedentary DM‐induced (DM + INS) and exercise training and insulin‐treated DM‐induced (DM + INS + EX) groups. Following DM induction, the 6‐week exercise training intervention (30 min of moderate‐intensity running on a treadmill, once daily [5 days/week]) was considered in EX groups. The tubular differentiation (TDI) and spermiogenesis (SPI) indices, testicular total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GPX) contents, serum testosterone and insulin levels, the apoptosis ratio and sperm parameters were assessed. The exercise in sole (EX) and simultaneous forms with INS (DM + INS + EX group) ameliorated the DM‐suppressed spermatogenesis and spermiogenesis indices, up‐regulated the serum testosterone and insulin levels, enhanced testicular SOD content, inhibited the apoptosis and improved almost all sperm parameters. In conclusion, exercise training, when simultaneously considered with insulin, fairly boosts the insulin‐induced impacts, including the up‐regulated testicular endocrine and antioxidant status, spermatogenesis and sperm quality.

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Section: However This Situation Was Reversed In Dm + Ins and Dm + Insupporting

confidence: 88%

Moderate‐intensity exercise training ameliorates the diabetes‐suppressed spermatogenesis and improves sperm parameters: Insole and simultaneous with insulin

et al. 2019

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“…Nonetheless, there are seasonal, management, hygienic and individual factors, such as rams' and ewes' breed, age and inseminator's expertise, that 2015), or no beneficial effect of cysteine to semen cryopreservation (Coyan et al, 2011). Furthermore, Sangeeta et al (2015) demonstrated that the addition of 20 mM l-glutamine and 25 mM l-proline in a Tris-egg yolk-glycerol diluent, reduced lipid peroxidation and significantly improved sperm motility, viability and acrosome integrity. In contrast, supplementation of l-alanine in freezing extender resulted in lower cryoprotection, observed by a decrease of sperm motility and an increase of the immotile and non-progressive spermatozoa percentage.…”

Section: Discussionmentioning

confidence: 98%

Current status and advances in ram semen cryopreservation

Ntemka

Tsakmakidis

Kiossis

et al. 2018

J Hellenic Vet Med Soc

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Ram semen cryopreservation contributes to genetic improvement through artificial insemination, eliminates geographical barriers in artificial insemination application and supports the preservation of endangered breeds thus the conservation of biodiversity. Sperm freezing process induces ultrastructural, biochemical and functional changes of spermatozoa. Especially, spermatozoa’s membranes and chromatin can be damaged, sperm membranes’ permeability is increased, hyper oxidation and formation of reactive oxygen species takes place, affecting fertilizing ability and subsequent early embryonic development. Aiming to improve ram frozen-thawed semen’s fertilizing capacity, many scientific investigations took place. Among them the composition of semen extenders, was a main point of interest. Semen preservation extenders regulate and support an environment of adequate pH and buffering capacity to protect spermatozoa from osmotic and cryogenic stress. Therefore, permeating (glycerol, dimethyl sulfoxide) and non-permeat ing (egg yolk, skimmed milk) cryoprotectants, sugars (glucose, lactose, trehalose, raffinose), salts (sodium citrate, citric acid) and antioxidants (amino acids, vitamins, enzymes) have been added and tested. Moreover, semen dilution rate, storage temperature, cooling rate and thawing protocol, are also some key factors that have been studied. The research results of this scientific topic are encouraging, not only about the freezing and thawing procedures, but also about the improvement of the additives’ properties. However, further research is needed to enhance the fertilizing ability of ram frozen-thawed semen, making its use practical in sheep reproductive management by the application of cervical artificial insemination.

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“…It might be a logical conclusion that DON via mitochondrial disturbance results in iROS production and the excessive generation of ROS along with oxidation of sperm membrane lipids affect the sperm membrane integrity and functionality. Indeed, the exogenously produced pathologic levels of ROS are able to adversely affect the sperm viability and motility by initiating massive lipid peroxidation of high polyunsaturated fatty acids (PUFA) within the sperm membrane (Sangeeta, Arangasamy, Kulkarni, & Selvaraju, 2015). Moreover, free radicals are able to potentially cross the cell membrane and inhibit the glucose-6-phosphate dehydrogenase (G6PD) activity, which in turn ultimately ends with a diminished availability of intracellular nicotinamide adenine dinucleotide phosphate (NADPH), as well as pathologic accumulation of oxidised glutathione (Zhang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Deoxynivalenol reduces quality parameters and increases DNA damage in mice spermatozoa

et al. 2019

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This study was performed to investigate in vitro effects of deoxynivalenol (DON) on mice sperm quality parameters including viability, motility and DNA damages at various concentrations and exposure times. Mice spermatozoa were exposed to DON at 0, 2.5, 5 and 10 µM for 1, 3 and 6 hr, motility parameters were evaluated by computer‐assisted analysis and viability was examined by colorimetric metabolic activity assay and HOS test. DNA damage was examined by acridine orange staining, and sperm damages via lipid peroxidation pathway were determined by malondialdehyde (MDA) content measurement. DON affected sperm parameters in a concentration‐ and time‐dependent manner. In all test groups, the average path velocity and progressive motile spermatozoa were remarkably reduced. In comparison with the controls, after 1, 3 and 6 hr exposure to DON, viability of spermatozoa was reduced 25, 30 and 49% respectively. DON exposure at 10 µM for 6 hr resulted in 15% DNA damage and 2.5‐fold more MDA generation, when compared with nonexposed spermatozoa. Our data suggest that DON causes sperm quality parameters decline in concentration‐ and time‐dependent fashion, which attribute to the reduction in sperm metabolic activity and membrane integrity and equally to increase in lipid peroxidation rate and DNA damage.

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Role of amino acids as additives on sperm motility, plasma membrane integrity and lipid peroxidation levels at pre-freeze and post-thawed ram semen

Cited by 53 publications

References 24 publications

Moderate‐intensity exercise training ameliorates the diabetes‐suppressed spermatogenesis and improves sperm parameters: Insole and simultaneous with insulin

Moderate‐intensity exercise training ameliorates the diabetes‐suppressed spermatogenesis and improves sperm parameters: Insole and simultaneous with insulin

Current status and advances in ram semen cryopreservation

Deoxynivalenol reduces quality parameters and increases DNA damage in mice spermatozoa

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