Role of Activator Protein 1 Transcriptional Activity in the Regulation of Gene Expression by Transforming Growth Factor β1 and Bone Morphogenetic Protein 2 in ROS 17/2.8 Osteoblast-like Cells

Abstract

In osteoblastic cells, transforming growth factor ␤1 (TGF-␤1) has been found to regulate the expression of a variety of proto-oncogenes including c-fos, c-jun, and junB. The c-fos in particular has been implicated in the mitogenic effect of TGF-␤1. Here, we examined the role of these early response genes in the regulation of osteoblast (

BMP-2-induced Runx2 Expression Is Mediated by Dlx5, and TGF-β1 Opposes the BMP-2-induced Osteoblast Differentiation by Suppression of Dlx5 Expression

“…Although we could not exclude the involvement of the other AP-1 components, we can suggest c-Jun is a good candidate for the mediator of antagonistic action of TGF-␤1 by following reasons; first, we showed c-Jun protein level was stimulated by TGF-␤1 but not by BMP-2. Second, not only the c-Jun but some other AP-1 components also could suppress Dlx5 expression even in the absence of TGF-␤1 (data not shown), but most of them were commonly stimulated by BMP-2 and TGF-␤1 treatment (28,39). Further studies would be required for a comprehensive understanding of the involvement of other AP-1 components and upstream signaling pathways.…”

Quercetin promotes the osteogenic differentiation of rat mesenchymal stem cells via mitogen-activated protein kinase signaling

“…In addition, TGF-β1 has been reported to induce the expression of specific genes, such as BGP, COL I and ALP (20). Therefore, TGF-β1 and BMP-2 play important roles in the osteoblastic differentiation of MSCs (21,22).…”

.BETA.-Cryptoxanthin and Bone Metabolism: The Preventive Role in Osteoporosis

“…IGF-I or TGF-β1 is a bone growth factors produced from osteoblasts. 27,28) The stimulatory effect of β-cryptoxanthin on the proliferation of osteoblastic cells may be partly mediated through the action of IGF-I or TGF-β1 produced from the cells.…”