Role of a sensor histidine kinase ChiS of Vibrio cholerae in pathogenesis

Chourashi, Rhishita; Mondal, Moumita; Sinha, Ritam; Debnath, A. K.; Das, Suman; Koley, Hemanta; Chatterjee, Nando K.

doi:10.1016/j.ijmm.2016.09.003

Cited by 15 publications

(24 citation statements)

References 35 publications

(44 reference statements)

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“…cholerae OMV specific antibody significantly reduce adherence by inhibiting V . cholerae motility [ 82 ]. So, our data indicate that our immunogen also induced protective immune response by blocking initial bacterial entry via intestinal epithelial cells.…”

Section: Resultsmentioning

confidence: 99%

Development of a novel S. Typhi and Paratyphi A outer membrane vesicles based bivalent vaccine against enteric fever

et al. 2018

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Salmonella Typhi and Salmonella Paratyphi A are the leading causative agents of enteric fever which cause morbidity and mortality worldwide. Currently, there is no combination vaccine which could protect infection from both the strains. In this paper, we are focusing on the development of a novel bivalent typhoidal Outer Membrane Vesicles (OMVs) based immunogen against enteric fever. We have isolated Salmonella Typhi and Paratyphi A OMVs and also characterized OMVs associated antigens. Then we immunized adult mice with three doses of our newly formulated bivalent immunogen orally (25 μg/200 μl). After three doses of oral immunization, we found our immunogen could significantly induce humoral response. We have also found serum IgG against LPS, Vi-polysaccharide etc. OMV immunization induces CD4, CD8 and CD19 population in immunized mice spleen. It also induces Th1 and Th17-cell mediated immunity. We also found bivalent OMVs immunization can prevent more than lethal dose of heterologous Salmonella strains mediated systemic infection in adult mice model. We determined that, the protective immune responses depend on the humoral and cell-mediated immune response. Furthermore, we have evaluated the mode of protective immune response carried out by anti-OMVs antibody by significantly inhibiting bacterial motility and mucin penetration ability. Taken together, these findings suggest that our bivalent immunogen could be used as a novel candidate vaccine against enteric fever.

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Section: Resultsmentioning

confidence: 99%

Development of a novel S. Typhi and Paratyphi A outer membrane vesicles based bivalent vaccine against enteric fever

et al. 2018

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“…Mucin also activates the two-component sensor histidine kinase ChiS in V. cholera . ChiS is the regulator of the chitinases and the chitin utilization pathway, but also plays a role in the virulence of the bacteria since the mutant strain is hypovirulent (Chourashi et al, 2016 ). Released monosaccharides from mucin O-glycans degradation can also act as a chemical cue to help pathogens to sense their environment and adapt accordingly.…”

Section: Role Of Mucin Components In Modulation Of Bacterial Virulencmentioning

confidence: 99%

Interactions of Intestinal Bacteria with Components of the Intestinal Mucus

Sicard

Bihan

Vogeleer

et al. 2017

Front. Cell. Infect. Microbiol.

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The human gut is colonized by a variety of large amounts of microbes that are collectively called intestinal microbiota. Most of these microbial residents will grow within the mucus layer that overlies the gut epithelium and will act as the first line of defense against both commensal and invading microbes. This mucus is essentially formed by mucins, a family of highly glycosylated protein that are secreted by specialize cells in the gut. In this Review, we examine how commensal members of the microbiota and pathogenic bacteria use mucus to their advantage to promote their growth, develop biofilms and colonize the intestine. We also discuss how mucus-derived components act as nutrient and chemical cues for adaptation and pathogenesis of bacteria and how bacteria can influence the composition of the mucus layer.

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“…Reports have also indicated the involvement of bacterial chitinases in pathogenesis [9][10][11]. Our studies have shown that the chitinase ChiA2 (VCA0027) is important for V. cholerae survival and pathogenesis [11,12].…”

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confidence: 72%

“…ChiS also promotes motility, mucin-layer penetration, adherence and survival in the intestinal environment. Moreover, ChiS mutant V. cholerae shows hypovirulent characteristics as it secretes less cholera toxin (CT) in the intestine, and hence shows less fluid accumulation and less virulence [12].…”

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confidence: 99%

Multifunctional transcription factor CytR of Vibrio cholerae is important for pathogenesis

et al. 2020

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Vibrio cholerae, the Gram-negative facultative pathogen, resides in the aquatic environment and infects humans and causes diarrhoeagenic cholera. Although the environment differs drastically, V. cholerae thrives in both of these conditions aptly and chitinases play a vital role in their persistence and nutrient acquisition. Chitinases also play a role in V. cholerae pathogenesis. Chitinases and its downstream chitin utilization genes are regulated by sensor histidine kinase ChiS, which also plays a significant role in pathogenesis. Recent exploration suggests that CytR, a transcription factor of the LacI family in V. cholerae, also regulates chitinase secretion in environmental conditions. Since chitinases and chitinase regulator ChiS is involved in pathogenesis, CytR might also play a significant role in pathogenicity. However, the role of CytR in pathogenesis is yet to be known. This study explores the regulation of CytR on the activation of ChiS in the presence of mucin and its role in pathogenesis. Therefore, we created a CytR isogenic mutant strain of V. cholerae (CytR¯) and found considerably less β-hexosaminidase enzyme production, which is an indicator of ChiS activity. The CytR¯ strain greatly reduced the expression of chitinases chiA1 and chiA2 in mucin-supplemented media. Electron microscopy showed that the CytR¯ strain was aflagellate. The expression of flagellar-synthesis regulatory genes flrB, flrC and class III flagellar-synthesis genes were reduced in the CytR¯ strain. The isogenic CytR mutant showed less growth compared to the wild-type in mucin-supplemented media as well as demonstrated highly retarded motility and reduced mucin-layer penetration. The CytR mutant revealed decreased adherence to the HT-29 cell line. In animal models, reduced fluid accumulation and colonization were observed during infection with the CytR¯ strain due to reduced expression of ctxB, toxT and tcpA. Collectively these data suggest that CytR plays an important role in V. cholerae pathogenesis.

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Role of a sensor histidine kinase ChiS of Vibrio cholerae in pathogenesis

Cited by 15 publications

References 35 publications

Development of a novel S. Typhi and Paratyphi A outer membrane vesicles based bivalent vaccine against enteric fever

Development of a novel S. Typhi and Paratyphi A outer membrane vesicles based bivalent vaccine against enteric fever

Interactions of Intestinal Bacteria with Components of the Intestinal Mucus

Multifunctional transcription factor CytR of Vibrio cholerae is important for pathogenesis

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