Role of a hisU gene in the control of stable RNA synthesis in Salmonella typhimurium

Davidson, John P.; Wilson, Donella J.; Williams, Luther S.

doi:10.1016/0022-2836(82)90232-7

Cited by 8 publications

(7 citation statements)

References 33 publications

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“…The spoT gene has been identified in S. typhimurium (276), and SpoT hydrolase activity in Bacillus subtilis (18) and Bacillus stearothermophilus (77) has been reported. In organisms in which the stringent response has been examined, such as S. typhimurium (58,59), B. subtilis (91,229,298,313), and Streptomyces coelicolor (310), the anatomy of the response has been shown to be very similar to that of E. coli. Interestingly, the original B. subtilis relA mutant isolated (313) has lost its ability to respond to both amino acid and carbon source starvation (229), and an intragenic suppressor mutation which selectively restores the response to carbon limitation has been isolated (113).…”

Section: Stringent Control In Other Organismsmentioning

confidence: 99%

“…Furthermore, data from the same group have shown that transcription elongation rate varies by only a few percent over VOL. 59,1995 CONTROL OF rRNA TRANSCRIPTION IN E. COLI the range of ppGpp concentrations normally found under steady-state growth conditions (302). From in vitro measurements at ppGpp concentrations similar to in vivo steady-state pools, the inhibitory effects of ppGpp on mRNA elongation rate were initially assumed to also apply to rRNA transcription (104,161,163).…”

Section: Ppgpp Modelsmentioning

confidence: 99%

“…There is a 1,000-fold induction of Fis upon subculturing of stationary-phase cells VOL. 59,1995 CONTROL OF rRNA TRANSCRIPTION IN E. COLI…”

Section: Does Fis Have a Regulatory Role?mentioning

confidence: 99%

“…Using a promoter fusion test system, Berg et al (22) have more precisely defined the regions necessary for rrn antitermination in vivo by saturation VOL. 59,1995 CONTROL OF rRNA TRANSCRIPTION IN E. COLI mutagenesis of the nut-like region. Consistent with the evidence presented by Gourse et al 106, a wild-type rrn boxA was both necessary and sufficient for readthrough of a strong rho-dependent terminator (22).…”

Section: Properties Of Antiterminationmentioning

confidence: 99%

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Control of rRNA transcription in Escherichia coli.

Condon¹,

Squires²

1995

Microbiological Reviews

238

162

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Section: Stringent Control In Other Organismsmentioning

confidence: 99%

Section: Ppgpp Modelsmentioning

confidence: 99%

“…There is a 1,000-fold induction of Fis upon subculturing of stationary-phase cells VOL. 59,1995 CONTROL OF rRNA TRANSCRIPTION IN E. COLI…”

Section: Does Fis Have a Regulatory Role?mentioning

confidence: 99%

Section: Properties Of Antiterminationmentioning

confidence: 99%

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Control of rRNA transcription in Escherichia coli.

Condon¹,

Squires²

1995

Microbiological Reviews

238

162

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“…Conversely, no effect on expression of the trp operon was observed under growth conditions expected to either induce or repress the operon. Deregulation of the his and ilv operons, but not the trp operon, has been reported for cells with reduced negative supercoiling (e.g., the hisW class of alleles) (8,10,27). As predicted from the derepression of the his operon, strain DM8306 (gyrA751) formed wrinkled colonies on medium with 2% glucose and was resistant to the histidine analog amino triazole (22,27).…”

mentioning

confidence: 95%

An Allele of gyrA Prevents Salmonella enterica Serovar Typhimurium from Using Succinate as a Carbon Source

Schmitz

Downs

2006

J Bacteriol

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A mutant gyrA allele resulting in an A271E substitution in the DNA gyrase protein generated a strain unable to grow on the C 4 -dicarboxylates succinate, malate, and fumarate. Bacteria harboring gyrA751 displayed decreased negative supercoiling in cells. Expression of the dctA gene, which encodes the C 4 -dicarboxylate transporter, was reduced in a gyrA751 mutant, providing the first evidence that dctA expression is supercoiling sensitive and uncovering a simple metabolic screen for lesions in gyrase that reduce negative supercoiling.

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his operons of Escherichia coli and Salmonella typhimurium are regulated by DNA supercoiling.

Rudd¹,

Menzel²

1987

Proc. Natl. Acad. Sci. U.S.A.

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The hisW mutations of Salmonella typhimurium are highly pleiotropic mutations that elevate his operon expression, reduce ilv gene expression, alter stable RNA metabolism, and confer defective growth properties. The hisW mutations are highly linked to a naladixic acid-resistant gyrA mutation of S. typhimurium. Multicopy recombinant plasmids containing the Escherichia coli gyrA gene are able to complement both the growth defects and the elevated his operon expression associated with the hisW mutations. We conclude that hisW mutations are alleles of the gyrA gene. The hisUl820 mutant of S. typhimurium exhibits many of the same phenotypes as hisW mutants. Several lines of evidence, including high transduction linkage to recF, suggest that hisUl820 is an allele of gyrB. Finally, well-characterized gyrA and gyrB alleles of E. coli are also his regulatory mutations. We propose that a wild-type degree of chromosomal superhelicity is required for maximal production of histidyl-tRNA and normal his operon regulation.Regulatory mutations at several loci (hisR,S,T, U, W) that are unlinked to the his operon result in the increased expression of the his operon in Salmonella typhimurium and Escherichia coli (1-3). These mutations all affect histidyl-tRNA metabolism, resulting in either lowered levels of histidyl-tRNA or, in one case (hisT), undermodified histidyl-tRNA (3)(4)(5). When the intracellular level of histidyl-tRNA is low, translation of the his leader peptide is inefficient, impeding formation ofthe his attenuator and allowing increased transcription of the his structural genes (6).Mutations hisW3333(Cs)(cold sensitive, lethal) and hisU-1820(Ts)(thermosensitive, lethal) of S. typhimurium are highly pleiotropic. In addition to the his regulatory phenotype, these mutants have lowered levels of several tRNA species (including histidyl-tRNA) (4), reduced levels of enzymes involved in isoleucine-valine biosynthesis (7,8), and are defective in the normal control of stable RNA accumulation (9)(10)(11). We show that the S. typhimurium hisW mutations are alleles of gyrA, the structural gene for the A subunit of DNA gyrase. In addition, we provide evidence that the his U1820-(Ts) mutation is an allele ofthe gyrB gene, the structural gene for the B subunit of DNA gyrase. Further, we were able to show that well-characterized gyrA and gyrB alleles of E. coli are his constitutive regulatory mutations causing phenotypes similar to those of the hisW3333(Cs) and hisU1820(Ts) mutations of S. typhimurium.DNA gyrase is the enzyme responsible for maintaining the bacterial chromosome in a state of negative superhelicity (12,13). Chromosomal superhelicity has been implicated in the control of gene expression since the activities of many promoters are modulated by changes in the superhelical density of the template DNA both in vivo and in vitro (reviewed in refs. 13-15). In particular, the hisR multigene tRNA operon (encoding the sole S. typhimurium tRNAHiS gene) is transcribed efficiently in vitro only when the DNA template is super...

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Role of a hisU gene in the control of stable RNA synthesis in Salmonella typhimurium

Cited by 8 publications

References 33 publications

Control of rRNA transcription in Escherichia coli.

Control of rRNA transcription in Escherichia coli.

An Allele of gyrA Prevents Salmonella enterica Serovar Typhimurium from Using Succinate as a Carbon Source

his operons of Escherichia coli and Salmonella typhimurium are regulated by DNA supercoiling.

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