Role of 830 nm Low Reactive Level Laser on the Growth of an Implanted Glioma in Mice.

Abe, Mamoru; Fujisawa, Kazuhisa; Suzuki, Hiromi; Sugimoto, T.; Kanno, Tetsuo

doi:10.2302/kjm.42.177

Cited by 15 publications

(13 citation statements)

References 4 publications

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“…Despite the stimulatory effects of laser on tissue repair, investigators have applied it at a wide variety of doses, which makes it difficult to compare published results and determine the best laser parameters for evoking the best tissue response [15][16][17][18]. Therefore, it is important to examine the effects of different fluences of LLLT to define its safety and efficiency.…”

Section: Introductionmentioning

confidence: 99%

Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion

Rodrigues

Assis²,

Fernandes³

et al. 2013

J Rehabil Res Dev

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Abstract-The aim of this study was to evaluate the effects of 660 nm low-level laser therapy (LLLT) on muscle regeneration after cryolesion in rat tibialis anterior muscle. Sixty-three Wistar rats were divided into a control group, 10 J/cm 2 lasertreated group, and 50 J/cm 2 laser-treated group. Each group formed three subgroups (n = 7 per group), and the animals were sacrificed 7, 14, or 21 d after lesion. Histopathological findings revealed a lower inflammatory process in the lasertreated groups after 7 d. After 14 d, irradiated animals at both fluences showed higher granulation tissue, new muscle fibers, and organized muscle structure. After 21 d, full tissue repair was observed in all groups. Moreover, irradiated animals at both fluences showed smaller necrosis area in the first experimental period evaluated. MyoD immunoexpression was observed in both treated groups 7 d postinjury. Myogenin immunoexpression was detected after 7 and 14 d. The higher fluence increased the number of blood vessels after 14 and 21 d. These results suggest that LLLT, at both fluences, positively affects injured skeletal muscle in rats, accelerating the muscle-regeneration process.

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Section: Introductionmentioning

confidence: 99%

Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion

Rodrigues

Assis²,

Fernandes³

et al. 2013

J Rehabil Res Dev

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“…The cellular proliferative potential of LLLT irradiation has attracted some negative speculation that this could also increase tumor growth in neoplasic diseases. Previous studies of LLLT irradiation of tumor cells in vitro have generated conflicting research data across a range of cultivated tumor cell lines and irradiation parameters [2-11] but there have been relatively few in vivo studies published [12,13]. In vivo studies are essential for the study of disease development and should be the main tool for studying the behavior of tumor cells.…”

Section: Introductionmentioning

confidence: 99%

The effect of low-level laser irradiation (In-Ga-Al-AsP - 660 nm) on melanoma in vitro and in vivo

et al. 2009

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BackgroundIt has been speculated that the biostimulatory effect of Low Level Laser Therapy could cause undesirable enhancement of tumor growth in neoplastic diseases. The aim of the present study is to analyze the behavior of melanoma cells (B16F10) in vitro and the in vivo development of melanoma in mice after laser irradiation.MethodsWe performed a controlled in vitro study on B16F10 melanoma cells to investigate cell viability and cell cycle changes by the Tripan Blue, MTT and cell quest histogram tests at 24, 48 and 72 h post irradiation. The in vivo mouse model (male Balb C, n = 21) of melanoma was used to analyze tumor volume and histological characteristics. Laser irradiation was performed three times (once a day for three consecutive days) with a 660 nm 50 mW CW laser, beam spot size 2 mm2, irradiance 2.5 W/cm2 and irradiation times of 60s (dose 150 J/cm2) and 420s (dose 1050 J/cm2) respectively.ResultsThere were no statistically significant differences between the in vitro groups, except for an increase in the hypodiploid melanoma cells (8.48 ± 1.40% and 4.26 ± 0.60%) at 72 h post-irradiation. This cancer-protective effect was not reproduced in the in vivo experiment where outcome measures for the 150 J/cm2 dose group were not significantly different from controls. For the 1050 J/cm2 dose group, there were significant increases in tumor volume, blood vessels and cell abnormalities compared to the other groups.ConclusionLLLT Irradiation should be avoided over melanomas as the combination of high irradiance (2.5 W/cm2) and high dose (1050 J/cm2) significantly increases melanoma tumor growth in vivo.

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“…15,17 In these studies, red light of He-Ne or ruby lasers (633 and 694 nm, respectively) were used as irradiation source. In these studies, the course of irradiations used was of different durations and irradiation doses varied from 40 to 70 J/cm 2 .…”

Section: Discussionmentioning

confidence: 99%

“…13 Low-power laser irradiation can stimulate the growth of transplanted malignant tumors in laboratory animals, 14 but many more studies demonstrate either the absence of the stimulating effect of light on this process, or its inhibition. 15,16 The maximal antitumor effect developed either after direct irradiation of tumors, 15,17 or after irradiation in animals in the area of tumor transplantation. 15 A possible cause for change in the tumor growth rate might be a reorganization of the tumor cell cytoskeleton.…”

Section: Introductionmentioning

confidence: 99%

“…15,16 The maximal antitumor effect developed either after direct irradiation of tumors, 15,17 or after irradiation in animals in the area of tumor transplantation. 15 A possible cause for change in the tumor growth rate might be a reorganization of the tumor cell cytoskeleton. 18,19 Indeed, after irradiation with IR using an 808 nm GaAlAs laser, at doses of 5.85 and 7.8 J/cm 2 there was a disturbance of the regular structure of cell microtubules of human hepatoma cells in culture.…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of Tumorogenicity and Changes in the Actin Cytoskeleton of Murine Hepatoma After Irradiation with Polychromatic Visible and IR Light

Knyazev

Samoilova

Abrahamse

et al. 2015

Photomedicine and Laser Surgery

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Role of 830 nm Low Reactive Level Laser on the Growth of an Implanted Glioma in Mice.

Cited by 15 publications

References 4 publications

Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion

Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion

The effect of low-level laser irradiation (In-Ga-Al-AsP - 660 nm) on melanoma in vitro and in vivo

Downregulation of Tumorogenicity and Changes in the Actin Cytoskeleton of Murine Hepatoma After Irradiation with Polychromatic Visible and IR Light

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