Robustness testing of live attenuated rubella vaccine potency assay using fractional factorial design of experiments

Kutle, Leonida; Pavlović, Nediljko; Dorotić, Marko; Zadro, Ivana; Kapustić, Marijana; Halassy, Beata

doi:10.1016/j.vaccine.2010.04.111

Cited by 9 publications

(9 citation statements)

References 6 publications

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“…The concentration of live RV in samples before and after chromatography was determined by virus titration in RK13 cells [26][27][28]. Viral samples were assayed in parallel with a working reference preparation calibrated against the International Reference Reagent for Rubella Virus Vaccine (Live) (NIBSC, South Mimms, UK), as a mean of ensuring reproducibility of the test results.…”

Section: Determination Of Concentration Of Live Rubella Virus By 50% mentioning

confidence: 99%

Concentration and purification of rubella virus using monolithic chromatographic support

Forčić

Brgles

Ivancic-Jelecki

et al. 2011

Journal of Chromatography B

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Section: Determination Of Concentration Of Live Rubella Virus By 50% mentioning

confidence: 99%

Concentration and purification of rubella virus using monolithic chromatographic support

Forčić

Brgles

Ivancic-Jelecki

et al. 2011

Journal of Chromatography B

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“…Rubella virus (RV) was titrated in microplates on RK 13 cells as described (Forcic et al 2011;Kutle et al 2010). …”

Section: Virus Titrationsmentioning

confidence: 99%

Stability of Minimum Essential Medium functionality despite l-glutamine decomposition

et al. 2015

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L-Glutamine (L-Gln) instability in liquid media is a well-known fact. Also, negative effect of ammonia, one of the L-Gln degradation products, on viability of many cell cultures and on replication of different viruses has been described. However, negative effects of ammonia have been reported in doses excessively exceeding those that could be generated in regularly used liquid culture media due to spontaneous L-Gln breakdown (below 2 mM). Traditional virus vaccine production processes have been established and registered involving L-Gln containing media use. Eventual culture media replacement in the regular production process belongs to the major regulative changes that require substantial financial expenses. The aim of this study was to evaluate the effect of storage of Minimum Essential Media with Hanks salts on their relevant biological functions during virus vaccine production process in relation to L-Gln decrease. Our results show a cell type dependent effect of spontaneous L-Gln degradation during medium storage. They also suggest that for cell cultures used in measles, mumps, and rubella virus production the media retain their functionality in respect to cell viability or virus growth over a certain time window despite L-Gln degradation.

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“…Recently, the DOE technique has been adopted in the design and testing of various applications including automotive assembly (Altayib, 2011), computational intelligence (Garcia, 2010, bioassay robustness studies (Kutlea, 2010) and many others. …”

Section: Optimization Of Induction Motors Using Design Of Experimentsmentioning

confidence: 99%

Optimization of Induction Motors Using Design of Experiments and Particle Swarm Optimization

Bouchekara¹,

Simsim²,

Anwari³

2012

Induction Motors - Modelling and Control

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Robustness testing of live attenuated rubella vaccine potency assay using fractional factorial design of experiments

Cited by 9 publications

References 6 publications

Concentration and purification of rubella virus using monolithic chromatographic support

Concentration and purification of rubella virus using monolithic chromatographic support

Stability of Minimum Essential Medium functionality despite l-glutamine decomposition

Optimization of Induction Motors Using Design of Experiments and Particle Swarm Optimization

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