Robustaflavone-4′-dimethyl ether from Selaginella uncinata attenuated lipopolysaccharide-induced acute lung injury via inhibiting FLT3-mediated neutrophil activation

Wu, Xiaoning; Yang, Yang; Zhang, Huan‐Huan; Zhong, Yu-Sen; Wu, Fang; Yu, Bing; Yu, Chunjing

doi:10.1016/j.intimp.2020.106338

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…Activated neutrophils enhance their chemotaxis and adhesion to endothelial cells, and migrate into the lung interstitium, which restricts the repair of lung tissues 45 . Inhibiting Fms-like tyrosine kinase 3-mediated activation of neutrophils was shown to alleviate LPS-induced ALI 46 . The results of the present study indicated that MSCs may alleviate the lung damage due to infiltrating neutrophils by inhibiting chemotaxis in Ly6C + CD8 + T cells.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells alleviate LPS-induced acute lung injury by inhibiting the proinflammatory function of Ly6C+ CD8+ T cells

Zhu

Feng

et al. 2020

Cell Death Dis

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Systemic inflammatory processes, including alveolar injury, cytokine induction, and neutrophil accumulation, play key roles in the pathophysiology of acute lung injury (ALI). The immunomodulatory effects of mesenchymal stem cells (MSCs) can contribute to the treatment of inflammatory disorders. In previous studies, the focus was on innate immune cells and the effects of MSCs on ALI through CD8+ T cells remain unclear. In the present study, lipopolysaccharide (LPS) was used to induce ALI in mice. ALI mice were treated with MSCs via intratracheal instillation. Survival rate, histopathological changes, protein levels, total cell count, cytokine levels, and chemokine levels in alveolar lavage fluid were used to determine the efficacy of MSCs. Mass cytometry and single-cell RNA sequencing (scRNA-seq) were used to characterize the CD8+ T cells in the lungs. Ly6C− CD8+ T cells are prevalent in normal mice, whereas a specialized effector phenotype expressing a high level of Ly6C is predominant in advanced disease. MSCs significantly mitigated ALI and improved survival. MSCs decreased the infiltration of CD8+ T cells, especially Ly6C+ CD8+ T cells into the lungs. Mass cytometry revealed that CD8+ T cells expressing high Ly6C and CXCR3 levels caused tissue damage in the lungs of ALI mice, which was alleviated by MSCs. The scRNA-seq showed that Ly6C+ CD8+ T cells exhibited a more activated phenotype and decreased expression of proinflammatory factors that were enriched the most in immune chemotaxis after treatment with MSCs. We showed that CD8+ T cells play an important role in MSC-mediated ALI remission, and both infiltration quantity and proinflammatory function were inhibited by MSCs, indicating a potential mechanism for therapeutic intervention.

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Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells alleviate LPS-induced acute lung injury by inhibiting the proinflammatory function of Ly6C+ CD8+ T cells

Zhu

Feng

et al. 2020

Cell Death Dis

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“…These findings showed that RDE could suppress FLT3 expression, then inhibit the LPS-induced AKT (Protein kinase B ) and MAPK pathways. In a dose-dependent way, RDE may reverse the elevated levels of P-selectin, IL-6, TNF- α , and intercellular adhesion molecule 1 (ICAM-1) caused by LPS stimulation and ameliorate lung pathologic characteristics [ 48 ].…”

Section: Effects Of Natural Products On Lps-induced Lung Injurymentioning

confidence: 99%

Effects of Herbs and Derived Natural Products on Lipopolysaccharide-Induced Toxicity: A Literature Review

Kianmehr

Behdadfard

Hedayati-Moghadam

et al. 2023

Oxidative Medicine and Cellular Longevity

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Introduction. Oxidative stress (OS) during inflammation can increase inflammatory responses and damage tissue. Lipopolysaccharide (LPS) can induce oxidative stress and inflammation in several organs. Natural products have several biological activities including anti-inflammatory, antioxidant, and immunoregulatory properties. The aims of the study are to study the possible therapeutic effects of natural products on LPS inducing toxicity on the nervous system, lung, liver, and immune system. Methods. The in vitro and in vivo research articles that were published in the last 5 years were included in the current study. The keywords included “lipopolysaccharide,” “toxicity,” “natural products,” and “plant extract” were searched in different databases such as Scopus, PubMed, and Google Scholar until October 2021. Results. The results of most studies indicated that some medicinal herbs and their potent natural products can help to prevent, treat, and manage LPS-induced toxicity. Medicinal herbs and plant-derived natural products showed promising effects on managing and treating oxidative stress, inflammation, and immunomodulation by several mechanisms. Conclusion. However, these findings provide information about natural products for the prevention and treatment of LPS-induced toxicity, but the scientific validation of natural products requires more evidence on animal models to replace modern commercial medicine.

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“…In addition, the other pharmacological effects of biflavonoids are: morelloflavone has 63% preventive inhibition of PLA2-induced myotoxic activity, its 38% cure rate inhibits myotoxicity, and it can inhibit edema formation and anticoagulation in a concentration-dependent manner, proving that morelloflavone can be developed as an inhibitor of secretory PLA2 such as in snake venom [ 309 ]; GB1 can inhibit α-glucosidase (IC 50 = 0.90 ± 0.01 mM) and aromatase (IC 50 = 0.28 ± 0.02 mM), and produce anti-plasmodium activity [ 310 ]; robustaflavone-4′-dimethyl ether can inhibit the accumulation of inflammatory cells by inhibiting the AKT and APK pathways, improve lung tissue damage, and reduce pulmonary edema [ 311 ]; rhusflavone, from Rhus parviflora, has a sedative and hypnotic effect, significantly binds to the GABAA-BZD receptor (IC 50 = 0.045 mM), and induces sleep [ 178 ]; II-3, I-5, II-5, II-7, I-4′,II-4′-hexahydroxy-(I-3,II-8)-flavonylflavanonol, from G arcinia nervosa var. pubescens King can produce 73.9% in 18.2 μg/mL Platelet-Activating-Factor Inhibition (IC 50 = 20.4 μM) [ 312 ]; and GB-2a-II-4′-OMe has a certain analgesic effect on the pain sensation induced by Marfrine, and its mechanism from analgesic effect is different of morphine [ 64 ]; amentoflavone can reduce the influence of gamma rays [ 313 ], and six biflavonoids of Araucaria angustifolia can improve DNA damage caused by ultraviolet radiation, including: amentoflavone, mono- O -methylamentoflavone, di- O -methylamentoflavone, ginkgetin, tri- O -methylamentoflavone, and tetra- O -methylamentoflavone [ 314 ]; GB-2a can inhibit the formation of melanin [ 315 ]; studies have shown that isoginkgetin is an inhibitor of mRNA splicing [ 316 ]; and chamaejasmine and ginkgetin can improve chronic dermatitis through anti-inflammatory effects [ 317 , 318 , 319 ].…”

Section: Pharmacology Of Biflavonoidsmentioning

confidence: 99%

Proceedings of Chemistry, Pharmacology, Pharmacokinetics and Synthesis of Biflavonoids

Yang

Huang

2021

Molecules

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Biflavonoids, composed of two monoflavonoid residues, occur naturally in angiosperms, bryophytes, ferns, and gymnosperms. More than 592 biflavonoids have been structurally elucidated, and they can be classified into two groups of C-C and C-linear fragments-C, based on whether the linker between the two residues contains an atom. As the linker can be established on two arbitrary rings from different residues, the C-C type contains various subtypes, as does the C-linear fragment-C type. Biflavonoids have a wide range of pharmacological activities, including anti-inflammatory, antioxidant, antibacterial, antiviral, antidiabetic, antitumor, and cytotoxic properties, and they can be applied in Alzheimer’s disease and Parkinson’s disease. This review mainly summarizes the distribution and chemistry of biflavonoids; additionally, their bioactivities, pharmacokinetics, and synthesis are discussed.

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Robustaflavone-4′-dimethyl ether from Selaginella uncinata attenuated lipopolysaccharide-induced acute lung injury via inhibiting FLT3-mediated neutrophil activation

Cited by 11 publications

References 19 publications

Mesenchymal stem cells alleviate LPS-induced acute lung injury by inhibiting the proinflammatory function of Ly6C+ CD8+ T cells

Mesenchymal stem cells alleviate LPS-induced acute lung injury by inhibiting the proinflammatory function of Ly6C+ CD8+ T cells

Effects of Herbs and Derived Natural Products on Lipopolysaccharide-Induced Toxicity: A Literature Review

Proceedings of Chemistry, Pharmacology, Pharmacokinetics and Synthesis of Biflavonoids

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