Robust silk fibroin/bacterial cellulose nanoribbon composite scaffolds with radial lamellae and intercalation structure for bone regeneration

Chen, Jian; Zhuang, Ao; Shao, Huili; Hu, Xuechao; Zhang, Yaopeng

doi:10.1039/c7tb00485k

Cited by 52 publications

(32 citation statements)

References 72 publications

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“…Cell proliferation on 1SF-1OBC was not significantly different from the TCPS control after 7 days. Similar results were also reported in BC/SF scaffolds, that exhibit biocompatibility (Chen et al 2017 ; Oliveira Barud et al 2015 ; Wang et al 2019 ). It can be seen from Fig.…”

Section: Resultssupporting

confidence: 82%

3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells

et al. 2020

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A novel biomaterial ink consisting of regenerated silk fibroin (SF) and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized bacterial cellulose (OBC) nanofibrils was developed for 3D printing lung tissue scaffold. Silk fibroin backbones were cross-linked using horseradish peroxide/H 2 O 2 to form printed hydrogel scaffolds. OBC with a concentration of 7wt% increased the viscosity of inks during the printing process and further improved the shape fidelity of the scaffolds. Rheological measurements and image analyses were performed to evaluate inks printability and print shape fidelity. Three-dimensional construct with ten layers could be printed with ink of 1SF-2OBC (SF/OBC = 1/2, w/w). The composite hydrogel of 1SF-1OBC (SF/OBC = 1/1, w/w) printed at 25 °C exhibited a significantly improved compressive strength of 267 ± 13 kPa and a compressive stiffness of 325 ± 14 kPa at 30% strain, respectively. The optimized printing parameters for 1SF-1OBC were 0.3 bar of printing pressure, 45 mm/s of printing speed and 410 μm of nozzle diameter. Furthermore, OBC nanofibrils could be induced to align along the print lines over 60% degree of orientation, which were analyzed by SEM and X-ray diffraction. The orientation of OBC nanofibrils along print lines provided physical cues for guiding the orientation of lung epithelial stem cells, which maintained the ability to proliferate and kept epithelial phenotype after 7 days’ culture. The 3D printed SF-OBC scaffolds are promising for applications in lung tissue engineering. Electronic supplementary material The online version of this article (10.1007/s10570-020-03526-7) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 82%

3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells

et al. 2020

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“…Biomedical sector -Further current developments in the BNC field mainly concern novel carrier materials for cell cultivation [122,137,138], encapsulation of cells/immobilization of enzymes [139], coating of medical devices [140], and the production of BNC materials using the well-known oxygen-transparent silicone supports [141,142]. In case of medical implants a lot of results is reported not only in the field of soft tissue implants (see Section In vivo scaffolds) but also regarding materials for bone regeneration [121,143,144].…”

Section: Recent Developments In Bnc Materialsmentioning

confidence: 99%

Nanocellulose as a natural source for groundbreaking applications in materials science: Today’s state

et al. 2018

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Nanocelluloses are natural materials with at least one dimension in the nano-scale. They combine important cellulose properties with the features of nanomaterials and open new horizons for materials science and its applications. The field of nanocellulose materials is subdivided into three domains: biotechnologically produced bacterial nanocellulose hydrogels, mechanically delaminated cellulose nanofibers, and hydrolytically extracted cellulose nanocrystals. This review article describes today's state regarding the production, structural details, physicochemical properties, and innovative applications of these nanocelluloses. Promising technical applications including gels/foams, thickeners/stabilizers as well as reinforcing agents have been proposed and research from last five years indicates new potential for groundbreaking innovations in the areas of cosmetic products, wound dressings, drug carriers, medical implants, tissue engineering, food and composites. The current state of worldwide commercialization and the challenge of reducing nanocellulose production costs are also discussed.

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“…Moreover, BC displays unique threedimensional nanofibrous network structure that resembles extracellular matrix (ECM) and high surface area with plenty of hydroxyl groups (Martínez, Brackmann, Enejder, & Gatenholm, 2012). Owning to the special properties and unique structure, BC has drawn much attention in application in biomedical areas (Picheth et al, 2017;Rajwade, Paknikar, & Kumbhar, 2015), for example, as implants for bone or cartilage tissue engineering (Chen, Zhuang, Shao, Hu, & Zhang, 2017;Kirdponpattara, Khamkeaw, Sanchavanakit, Pavasant, & Phisalaphong, 2015), as substitutes for skin repairing (Fu et al, 2012), and as supports for controlled drug delivery (Shi et al, 2012). However, native BC lacks the activity to inhibit bacteria growth on its surface, which limits its applications in biomedical fields.…”

Section: Introductionmentioning

confidence: 99%

Preparation of aminoalkyl‐grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications

Zhang

Zheng

et al. 2020

J Biomedical Materials Res

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Bacterial cellulose (BC) membranes display special properties and structures, thus attracting much attention in application in the biomedical areas, for example, as implants for bone or cartilage tissue engineering, as substitutes for skin repairing, and as supports for controlled drug delivery. However, native BC lacks the activity to inhibit bacteria growth on its surface, which limits its applications in biomedical fields. There have been reports on chemical modification of BC membranes to endow them with antimicrobial properties needed for some special biomedical applications. In the present study, aminoalkyl‐grafted BC membranes were prepared by alkoxysilane polycondensation using 3‐aminopropyltriethoxysilane (APTES). The characterization for morphology and chemical composition showed that BC membranes were successfully grafted with aminoalkylsilane groups through covalent bonding. The surface morphology and roughness of the membranes changed after chemical grafting. Furthermore, after grafting with APTES, the membranes got less hydrophilic than native BC. The aminoalkyl‐grafted BC membranes showed strong antibacterial properties against Staphylococcus aureus and Escherichia coli and moreover, they were nontoxic to normal human dermal fibroblasts. These results indicate that aminoalkyl‐grafted BC membranes are potential to be used for biomedical applications.

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Robust silk fibroin/bacterial cellulose nanoribbon composite scaffolds with radial lamellae and intercalation structure for bone regeneration

Abstract: Biomimetic scaffolds with a gradient gap distance and robust mechanical properties were prepared using silk fibroin and bacterial cellulose.

Cited by 52 publications

References 72 publications

3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells

3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells

Nanocellulose as a natural source for groundbreaking applications in materials science: Today’s state

Preparation of aminoalkyl‐grafted bacterial cellulose membranes with improved antimicrobial properties for biomedical applications

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