Robust bioactive protein-based screws with dual crosslinked network for internal bone fixation

Zhang, Qichen; Ding, Wang; Ding, Shenglong; Meng, Qingbing; Su, Dihan; Zhang, Tai-Wei; Chen, Qing; Lian, Ruixian; Zhao, Mengnan; Yu, Baoqing; Li, Xilei; Li, Yulin; Jiang, Libo

doi:10.1016/j.compositesb.2022.109884

Cited by 9 publications

(10 citation statements)

References 45 publications

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“…The electronic search initially identified 245 silk-related orthopedic/plastic surgery studies, which ultimately yielded 33 studies for full-text reviews (Figure 1). After thorough assessment and subsequent exclusion, 15 studies from 2014 to 2023 were included for quantitative analysis (Table S1) [6,[15][16][17][18][19][20][21][22][23][24][25][26][27][28]. These consisted of seven in vitro experimental studies and eight in vivo experimental studies.…”

Section: Resultsmentioning

confidence: 99%

“…Zhang et al developed silk fibroin/gelatin (RSF-G) screws and compared them with pure silk fibroin (RSF) screws in a rat model to evaluate their biocompatibility and strength [24]. They observed preliminary resorption and degradation of both RSF and RSF/G hydrogels at four weeks after implantation, with RSF/G hydrogels showing more degradation at eight weeks compared to RSF hydrogels [24].…”

Section: In Vivo Studiesmentioning

confidence: 99%

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Redefining Surgical Materials: Applications of Silk Fibroin in Osteofixation and Fracture Repair

Foppiani,

Taritsa,

Foster

et al. 2024

Biomimetics

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Silk and silk derivatives have emerged as a possible alternative in surgical device development, offering mechanical strength, biocompatibility, and environmental sustainability. Through a systematic review following PRISMA guidelines, this study evaluated silk fibroin’s application across pre-clinical and clinical settings, focusing on its role as screws and plates for osteofixation. A comprehensive search yielded 245 studies, with 33 subjected to full-text review and 15 ultimately included for qualitative analysis. The findings underscore silk fibroin’s superior properties, including its tunable degradation rates and ability to be functionalized with therapeutic agents. In vivo and in vitro studies demonstrated its efficacy in enhancing bone healing, offering improved outcomes in osteofixation, particularly for craniofacial defects. Silk fibroin’s remarkable attributes in biodegradation and drug release capabilities underscore its potential to enhance patient care. Ultimately, silk fibroin’s integration into surgical practices promises a revolution in patient outcomes and environmental sustainability. Its versatility, coupled with the continuous progress in fabrication techniques, signals a promising horizon for its widespread acceptance in the medical field, potentially establishing a new benchmark in surgical treatment. Further research is expected to solidify the transition of silk products from basic science to patient care, paving the way for widespread use in various surgical applications.

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Section: Resultsmentioning

confidence: 99%

Section: In Vivo Studiesmentioning

confidence: 99%

Redefining Surgical Materials: Applications of Silk Fibroin in Osteofixation and Fracture Repair

Foppiani,

Taritsa,

Foster

et al. 2024

Biomimetics

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“…With recent advances in processing, silk can now be engineered into mechanically robust, dense, plastic-like bulk materials with outstanding machinability, providing a path toward the fabrication of a new class of biodegradable orthopedic devices with good control over degradation rate. − By tuning the processing parameters, silk thermoplastic molding can generate bulk silk materials with molecular structures and physical properties required to fabricate silk bone screws and plates for implantation . Importantly, labile biomolecules can withstand the high process temperatures (>100 °C) and pressures (632 MPa) involved in generating these thermoplastic-molded silk materials, maintaining their activity.…”

Section: Introductionmentioning

confidence: 99%

Tuning the Biodegradation Rate of Silk Materials via Embedded Enzymes

Wu,

Cortes,

et al. 2024

ACS Biomater. Sci. Eng.

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Conventional thinking when designing biodegradable materials and devices is to tune the intrinsic properties and morphological features of the material to regulate their degradation rate, modulating traditional factors such as molecular weight and crystallinity. Since regenerated silk protein can be directly thermoplastically molded to generate robust dense silk plastic-like materials, this approach afforded a new tool to control silk degradation by enabling the mixing of a silk-degrading protease into bulk silk material prior to thermoplastic processing. Here we demonstrate the preparation of these silk-based devices with embedded silkdegrading protease to modulate the degradation based on the internal presence of the enzyme to support silk degradation, as opposed to the traditional surface degradation for silk materials. The degradability of these silk devices with and without embedded protease XIV was assessed both in vitro and in vivo. Ultimately, this new process approach provides direct control of the degradation lifetime of the devices, empowered through internal digestion via water-activated proteases entrained and stabilized during the thermoplastic process.

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“…28 The use of resorbable bone plates and screws composed of SF has garnered extensive interest as they obviate the need for implant removal and result in improved bone remodeling. 29 However, their manufacturing methods are still suffering from inefficient, time-consuming lathe machining 30 and difficulties in loading bioactive factors and cells for bone repair. The application of the photo-cross-linking SF hydrogel in the rapid fabrication of bone repair devices is expected to overcome these drawbacks.…”

Section: Introductionmentioning

confidence: 99%

Superb Silk Hydrogels with High Adaptability, Bioactivity, and Versatility Enabled by Photo-Cross-Linking

Huang,

Hua,

et al. 2024

ACS Nano

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The exceptional biocompatibility and adaptability of hydrogels have garnered significant interest in the biomedical field for the fabrication of biomedical devices. However, conventional synthetic hydrogels still exhibit relatively weak and fragile properties. Drawing inspiration from the photosynthesis process, we developed a facile approach to achieve a harmonious combination of superior mechanical properties and efficient preparation of silk fibroin hydrogel through photo-cross-linking technology, accomplished within 60 s. The utilization of riboflavin and H 2 O 2 enabled a sustainable cyclic photo-cross-linking reaction, facilitating the transformation from tyrosine to dityrosine and ultimately contributing to the formation of highly cross-linked hydrogels. These photo-cross-linking hydrogels exhibited excellent elasticity and restorability even after undergoing 1000 cycles of compression. Importantly, our findings presented that hydrogel-encapsulated adipose stem cells possess the ability to stimulate cell proliferation along with stem cell stemness. This was evidenced by the continuous high expression levels of OCT4 and SOX2 over 21 days. Additionally, the utilization of photo-cross-linking hydrogels can be extended to various material molding platforms, including microneedles, microcarriers, and bone screws. Consequently, this study offered a significant approach to fabricating biomedical hydrogels capable of facilitating real-time cell delivery, thereby introducing an innovative avenue for designing silk devices with exceptional machinability and adaptability in biomedical applications.

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Robust bioactive protein-based screws with dual crosslinked network for internal bone fixation

Cited by 9 publications

References 45 publications

Redefining Surgical Materials: Applications of Silk Fibroin in Osteofixation and Fracture Repair

Redefining Surgical Materials: Applications of Silk Fibroin in Osteofixation and Fracture Repair

Tuning the Biodegradation Rate of Silk Materials via Embedded Enzymes

Superb Silk Hydrogels with High Adaptability, Bioactivity, and Versatility Enabled by Photo-Cross-Linking

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