RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far

Joga, Mallikarjuna Reddy; Zotti, Moisés João; Smagghe, Guy; Christiaens, Olivier

doi:10.3389/fphys.2016.00553

Cited by 360 publications

(397 citation statements)

References 132 publications

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“…This work concentrates on nematode pests of banana, but the approach is equally applicable to any group of pests and pathogens for which common and effective target sequences can be identified. Genetic resources and screens for efficacy are available for the approach to be extended to insects (Joga et al ., ), viruses (Galvez et al ., ) and fungi (Salame et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Rational design of biosafe crop resistance to a range of nematodes using RNA interference

Roderick

Urwin

Atkinson

2017

Plant Biotechnology Journal

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SummaryDouble‐stranded RNA (dsRNA) molecules targeting two genes have been identified that suppress economically important parasitic nematode species of banana. Proteasomal alpha subunit 4 (pas‐4) and Actin‐4 (act‐4) were identified from a survey of sequence databases and cloned sequences for genes conserved across four pests of banana, Radopholus similis, Pratylenchus coffeae, Meloidogyne incognita and Helicotylenchus multicinctus. These four species were targeted with dsRNAs containing exact 21 nucleotide matches to the conserved regions. Potential off‐target effects were limited by comparison with Caenorhabditis, Drosophila, rat, rice and Arabidopsis genomes. In vitro act‐4 dsRNA treatment of R. similis suppressed target gene expression by 2.3‐fold, nematode locomotion by 66 ± 4% and nematode multiplication on carrot discs by 49 ± 5%. The best transgenic carrot hairy root lines expressing act‐4 or pas‐4 dsRNA reduced transcript message abundance of target genes in R. similis by 7.9‐fold and fourfold and nematode multiplication by 94 ± 2% and 69 ± 3%, respectively. The same act‐4 and pas‐4 lines reduced P. coffeae target transcripts by 1.7‐ and twofold and multiplication by 50 ± 6% and 73 ± 8%. Multiplication of M. incognita on the pas‐4 lines was reduced by 97 ± 1% and 99 ± 1% while target transcript abundance was suppressed 4.9‐ and 5.6‐fold. There was no detectable RNAi effect on nontarget nematodes exposed to dsRNAs targeting parasitic nematodes. This work defines a framework for development of a range of nonprotein defences to provide broad resistance to pests and pathogens of crops.

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Section: Discussionmentioning

confidence: 99%

Rational design of biosafe crop resistance to a range of nematodes using RNA interference

Roderick

Urwin

Atkinson

2017

Plant Biotechnology Journal

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“…The product was used as a food additive for wintering bees, with outstanding results regarding mortality and overall health. Topical spray delivery of dsRNA in planta has been reported to successfully target insect pests feeding on the plant . Soil applications for root absorption or trunk injections have also been addressed with positive results on gene silencing, confirming that plant root can take up dsRNA and that trunk injections facilitate the delivery of dsRNA through xylem and phloem …”

Section: Nano‐ and Bio‐technological Approaches For Developing A New mentioning

confidence: 99%

Safe nanotechnologies for increasing the effectiveness of environmentally friendly natural agrochemicals

Vurro

Miguel-Rojas

Pérez‐de‐Luque

2019

Pest Management Science

107

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Natural compounds and living organisms continue to play a limited role in crop protection, and few of them have reached the market, despite their attractiveness and the efforts made in research. Very often these products have negative characteristics compared to synthetic compounds, e.g., higher costs of production, lower effectiveness, lack of persistence, and inability to reach and penetrate the target plant. Conversely, nanotechnologies are having an enormous impact on all human activities, including agriculture, even if the production of some nanomaterials is not environmentally friendly or could have adverse effects on agriculture and the environment. Thus, certain nanomaterials could facilitate the development of formulated natural pesticides, making them more effective and more environmentally friendly. Nanoformulations can improve efficacy, reduce effective doses, and increase shelf‐life and persistence. Such controlled‐release products can improve delivery to the target pest. This review considers certain available nanomaterials and nanotechnologies for use in agriculture, discussing their properties and the feasibility of their use in sustainable crop protection, in particular, in improving the effectiveness of natural bio‐based agrochemicals. © 2019 Society of Chemical Industry

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“…By targeting essential genes, often those involved in development, RNAi has been shown to control several economically important pest insects including the western corn rootworm ( Diabrotica virgifera virgifera ), Colorado potato beetle ( Leptinotarsa decemlineata ) and several species of mosquitoes (Baum et al., ; Singh, Wong, Ryan, & Whyard, ; Zhu, Xu, Palli, Ferguson, & Palli, ). Several field‐based application strategies are available for dsRNA delivery, most involving the oral ingestion of nucleic acid via feeding on soluble/sprayable particles or transgenic plants (Joga, Zotti, Smagghe, & Christiaens, ; Zhang et al., ). However, the efficiency of RNAi varies in different insect species and feasibility studies are required to ensure that RNAi‐based strategies are effective in different insect pests.…”

Section: Novel Biotechnology‐based Pest Control Approaches—molecular mentioning

confidence: 99%

Environmentally sustainable pest control options for Drosophila suzukii

Schetelig

Lee

Otto

et al. 2017

J Applied Entomology

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The spotted-wing drosophila or cherry vinegar fly (Drosophila suzukii) is native to Asia but has invaded other continents since 2008 and has spread throughout Europe. The females have a serrated ovipositor allowing them to penetrate the skins of intact ripening fruits to deposit their eggs, and the developing larvae rapidly destroy the fruits close to harvest. Drosophila suzukii has a rapid life cycle and the larvae develop well beneath the fruit surface. This means that the use of pesticides is problematic and often not effective, first due to their restricted use close to harvest to protect consumers, and second because the larvae are deep enough inside the fruit to avoid contact.There are currently no effective and environmentally sustainable pest control methods for this species, resulting in extensive damage to fruit crops. Here, we review the current status of D. suzukii as a fruit crop pest and discuss the feasibility of current pesticide-free control methods. We also consider the potential of new technologies as a basis for the urgently needed specific and long-term control of this species. K E Y W O R D Scherry vinegar fly, integrated pest management, invasive insects, RNA interference, sterile insect technique

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RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far

Cited by 360 publications

References 132 publications

Rational design of biosafe crop resistance to a range of nematodes using RNA interference

Rational design of biosafe crop resistance to a range of nematodes using RNA interference

Safe nanotechnologies for increasing the effectiveness of environmentally friendly natural agrochemicals

Environmentally sustainable pest control options for Drosophila suzukii

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