RNAi down-regulation of cinnamate-4-hydroxylase increases artemisinin biosynthesis in Artemisia annua

Kumar, Ritesh; Vashisth, Divya; Misra, Ankita; Akhtar, Md. Qussen; Jalil, Syed Uzma; Shanker, Karuna; Gupta, Madan M.; Rout, Prasanta Kumar; Gupta, Anil Kumar; Shasany, Ajit Kumar

doi:10.1038/srep26458

Cited by 42 publications

(26 citation statements)

References 56 publications

(75 reference statements)

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“…Finally, Pg and Mv are synthesized from Cy and Dp, respectively, with the help of O-methyltransferase (OMT). Among the genes involved in anthocyanin synthesis, C4H is one of the core genes of the phenylpropanoid pathway and mediates the synthesis of secondary metabolites such as anthocyanins [6] and artemisinin [7]. CHS links the phenylpropanoid pathway and the flavonoid pathway as well as plays an important role in the biosynthesis of anthocyanins [8].…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Zhang

Zeng

et al. 2020

BMC Genomics

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Background: Anthocyanins are common substances with many agro-food industrial applications. However, anthocyanins are generally considered to be found only in natural plants. Our previous study isolated and purified the fungus Aspergillus sydowii H-1, which can produce purple pigments during fermentation. To understand the characteristics of this strain, a transcriptomic and metabolomic comparative analysis was performed with A. sydowii H-1 from the second and eighth days of fermentation, which confer different pigment production. Results: We found five anthocyanins with remarkably different production in A. sydowii H-1 on the eighth day of fermentation compared to the second day of fermentation. LC-MS/MS combined with other characteristics of anthocyanins suggested that the purple pigment contained anthocyanins. A total of 28 transcripts related to the anthocyanin biosynthesis pathway was identified in A. sydowii H-1, and almost all of the identified genes displayed high correlations with the metabolome. Among them, the chalcone synthase gene (CHS) and cinnamate-4hydroxylase gene (C4H) were only found using the de novo assembly method. Interestingly, the best hits of these two genes belonged to plant species. Finally, we also identified 530 lncRNAs in our datasets, and among them, three lncRNAs targeted the genes related to anthocyanin biosynthesis via cis-regulation, which provided clues for understanding the underlying mechanism of anthocyanin production in fungi. Conclusion: We first reported that anthocyanin can be produced in fungus, A. sydowii H-1. Totally, 31 candidate transcripts were identified involved in anthocyanin biosynthesis, in which CHS and C4H, known as the key genes in anthocyanin biosynthesis, were only found in strain H1, which indicated that these two genes may contribute to anthocyanins producing in H-1. This discovery expanded our knowledges of the biosynthesis of anthocyanins and provided a direction for the production of anthocyanin.

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Section: Introductionmentioning

confidence: 99%

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Zhang

Zeng

et al. 2020

BMC Genomics

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“…and amphidiploid were estimated using the method as described by Kumar et al [63]. The amphidiploids were compared with parent 1 (OBP1), parent 2 (OKP2) and interspeci c of F1…”

Section: Quantitative Rt-pcr For Gene Expression Analysismentioning

confidence: 99%

Basil interspecific hybridization and transcriptome study indicates altered developmental and metabolic gene expression

Shah¹,

Rastogi²,

Vashisth³

et al. 2020

Preprint

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Background In order to understand the developmental modulation of transcriptome and associated gene expression in inter-genomic combinations, a systematic study was planned using two diverse yet closely related species of Ocimum, targeting their hybrid F1 and derived amphidiploid (colchiploid of F1 hybrid). The existing developmental alterations between F1 and amphidiploid through phenotypical and anatomical assessments were analyzed. Results Study of several genes and transcription factors putatively involved in the growth and developmental processes of plants clearly amalgamates the transcriptome data linking the phenotypic differences in F1 and amphidiploid. Additionally, differentially expressed genes of stomatal patterning and development revealed their involvement leading to higher density of stomata in F1 while larger size of stomata in the amphidiploid. Absence of 8,330 transcripts of interspecific hybrid F1 in its amphidiploid and exclusive presence of two detected transcripts in amphidiploid provides a set of genes to analyze the suppressed or activated functions between F1 and amphidiploid. Estimation of chlorophyll, lignin, flavonoid and phenylpropenes (eugenol and methyleugenol) content were correlated with the average FPKM and digital gene expression values in F1 and amphidiploid. Conclusion This is the first investigation which describes the genes and transcription factors influenced by interspecific hybridization leading to developmental changes and alleviation of intergenomic instability in amphidiploid.

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“…Regardless of the victory of specific plant secondary metabolites substances as drugs, their restricted accessibility and challenging synthesis often make them only leads and not candidate drugs for scientists and pharmaceutical companies in the quest for new therapeutic hits. 81 With the increasing economic significance of secondary plant metabolites, enormous amount of efforts are put into research to understand and [48] Artemisia annua L.…”

Section: Commercial Developmentmentioning

confidence: 99%

Ascertaining the Paradigm of Secondary Metabolism Enhancement through Gene Level Modification in Therapeutic Plants

Sinha¹,

Sandhu²,

Bisht³

et al. 2019

JYP

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Since ages plants with therapeutic effects are used for curing several human ailments. With the development in modern biotechnological tools, these therapeutic compounds could be overexpressed in the plants. This review aims to compile the valuable information from the examples in the literature, regarding the biotechnological tools those could be useful for the overproduction of health-promoting biochemicals in the medicinal plants. Gene level modification is all about creating improved variety of plants that are highly resistant to pests and pesticides or contain higher levels of nutrients than conventional plants. Plant secondary metabolites constitute an exciting and vital aspect of research, due to their chemical diversity, varied biological functions and pharmacological activities. Gene modification, through Agrobacterium mediated transformation , RNA interference or CRISPER/Cas9, has opened avenues of improvement in medicinal plants and provide an alternative production system of pharmaceutically important secondary metabolites for their commercial exploitation.

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RNAi down-regulation of cinnamate-4-hydroxylase increases artemisinin biosynthesis in Artemisia annua

Cited by 42 publications

References 56 publications

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Basil interspecific hybridization and transcriptome study indicates altered developmental and metabolic gene expression

Ascertaining the Paradigm of Secondary Metabolism Enhancement through Gene Level Modification in Therapeutic Plants

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