RNA virus-like particles in the chytridiomycete Allomyces arbuscula

Khandjian, Édouard W.; Roos, Urs-Peter; Timberlake, William E.; Eder, Lorenza; Turian, G.

doi:10.1007/bf00455951

Cited by 6 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1 ). Known mycovirus infections in non-Dikarya are limited to an early report of virus-like particles from ultrastructure studies of Allomyces arbusculus ( 6 ) and sequence-based identification in Rhizopus oryzae ( 7 ), Rhizopus microsporus ( 8 ), six isolates of arbuscular mycorrhizal fungi ( 9 , 10 ), Umbelopsis ramanniana ( 11 ), and Entomophthora muscae ( 12 , 13 ). While it has become clear that mycoviruses are widespread, disproportionate sampling across the fungal kingdom has resulted in an incomplete understanding of mycovirus diversity and prevalence.…”

Section: Introductionmentioning

confidence: 99%

Survey of Early-Diverging Lineages of Fungi Reveals Abundant and Diverse Mycoviruses

Myers

Bonds

Clemons

et al. 2020

mBio

View full text Add to dashboard Cite

Mycoviruses are widespread and purportedly common throughout the fungal kingdom, although most are known from hosts in the two most recently diverged phyla, Ascomycota and Basidiomycota, together called Dikarya. To augment our knowledge of mycovirus prevalence and diversity in underexplored fungi, we conducted a large-scale survey of fungi in the earlier-diverging lineages, using both culture-based and transcriptome-mining approaches to search for RNA viruses. In total, 21.6% of 333 isolates were positive for RNA mycoviruses. This is a greater proportion than expected based on previous taxonomically broad mycovirus surveys and is suggestive of a strong phylogenetic component to mycoviral infection. Our newly found viral sequences are diverse, composed of double-stranded RNA, positive-sense single-stranded RNA (ssRNA), and negative-sense ssRNA genomes and include novel lineages lacking representation in the public databases. These identified viruses could be classified into 2 orders, 5 families, and 5 genera; however, half of the viruses remain taxonomically unassigned. Further, we identified a lineage of virus-like sequences in the genomes of members of Phycomycetaceae and Mortierellales that appear to be novel genes derived from integration of a viral RNA-dependent RNA polymerase gene. The two screening methods largely agreed in their detection of viruses; thus, we suggest that the culture-based assay is a cost-effective means to quickly assess whether a laboratory culture is virally infected. This study used culture collections and publicly available transcriptomes to demonstrate that mycoviruses are abundant in laboratory cultures of early-diverging fungal lineages. The function and diversity of mycoviruses found here will help guide future studies into mycovirus origins and ecological functions. IMPORTANCE Viruses are key drivers of evolution and ecosystem function and are increasingly recognized as symbionts of fungi. Fungi in early-diverging lineages are widespread, ecologically important, and comprise the majority of the phylogenetic diversity of the kingdom. Viruses infecting early-diverging lineages of fungi have been almost entirely unstudied. In this study, we screened fungi for viruses by two alternative approaches: a classic culture-based method and by transcriptome-mining. The results of our large-scale survey demonstrate that early-diverging lineages have higher infection rates than have been previously reported in other fungal taxa and that laboratory strains worldwide are host to infections, the implications of which are unknown. The function and diversity of mycoviruses found in these basal fungal lineages will help guide future studies into mycovirus origins and their evolutionary ramifications and ecological impacts.

show abstract

Section: Introductionmentioning

confidence: 99%

Survey of Early-Diverging Lineages of Fungi Reveals Abundant and Diverse Mycoviruses

Myers

Bonds

Clemons

et al. 2020

mBio

View full text Add to dashboard Cite

show abstract

“…En ce qui concerne la souche Bali 1 du Chytridiomyc6te Allomyces arbuscula, nous avons rapport6 l'inocuit6 apparente d'un mycovirus ~t ARN bicat6naire (4,5,6) en nous basant star l'absence de d6sordres cytopathologiques ~t l'exception des 'nids' viraux 6cartant les ribosomes du 'nuclear cap' (! 0).…”

Section: Introductionunclassified

Anomalies physio-morphologiques de la souche viros�e (Bali 1) d'Allomyces arbuscula

Khandjian

Turian

1981

Mycopathologia

Self Cite

View full text Add to dashboard Cite

An escape reaction of the hyphae from virus-containing strain Bali 1 ofA. arbuscula was detected when intra-and interspecific Allomyces cultures were opposed on solid media. Hyphae of this virus-containing strain proliferate into witch-broom structures only detectable on solid media in which bunches of rhizoid-like structures remain more superfcial than those of the control cultures, suggesting that metabolism of the Bali 1 strain is altered into a more aerobic type.

show abstract

Virus-like particles in the aquatic fungus, Rhizidiomyces

Dawe

Kuhn

1983

Virology

View full text Add to dashboard Cite

RNA virus-like particles in the chytridiomycete Allomyces arbuscula

Cited by 6 publications

References 13 publications

Survey of Early-Diverging Lineages of Fungi Reveals Abundant and Diverse Mycoviruses

Survey of Early-Diverging Lineages of Fungi Reveals Abundant and Diverse Mycoviruses

Anomalies physio-morphologiques de la souche viros�e (Bali 1) d'Allomyces arbuscula

Virus-like particles in the aquatic fungus, Rhizidiomyces

Contact Info

Product

Resources

About