Virus-like particles in the aquatic fungus, Rhizidiomyces

Dawe, Violet H.; Kuhn, C. W.

doi:10.1016/0042-6822(83)90113-7

Cited by 11 publications

(3 citation statements)

References 26 publications

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“…The similarities in morphology and fine structure of chlorella viruses with members of the family Iridoviridae has been described and discussed in the present study on CVG-1. However, research on the genome organisation of PBCV-1 [2] revealed a major difference between the chlorella viruses and iridoviruses. Studies on virus attachment to and penetration into the algal host cells indicate that the chlorella viruses not only differ from iridoviruses [6,15,19], but also differ from all the other DNA viruses that infect eukaryotes.…”

Section: Discussionmentioning

confidence: 97%

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Ultrastructural studies on a chlorella virus from Germany

Becker

Lesemann

Reißer

1993

Archives of Virology

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This is the first report on the morphology and fine structure of chlorella virus Göttingen-1 (CVG-1), an European member of the newly approved family Phycodnaviridae, which infects certain unicellular, eukaryotic, exsymbiotic, green algae. CVG-1 are polyhedral particles 145-160 nm in diameter. The capsid consists of two shells, apparently composed of ca. 7 nm subunits. Whereas the outer shell of the capsid appears to be icosahedral, the inner shell appears to be irregular underneath one vertex of the virion. In this vertex the inner shell is separated from the outer shell leaving a distinct space ("empty vertex") of unknown function.

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Section: Discussionmentioning

confidence: 97%

“…Particles fixed with osmium tetroxide only, contained disorganized, coarsely stranded material in the cores (Figs. 3 b and 6c); this material resembled the dsDNA-containing regions of similarly fixed bacterial cells [22] or mycoviral dsDNA [2] or dsRNA [31]. In Fig.…”

Section: Subunits Of the Capsidmentioning

confidence: 88%

Ultrastructural studies on a chlorella virus from Germany

Becker

Lesemann

Reißer

1993

Archives of Virology

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“…Our work on the multilayered ecological framework represented by P. oceanica, its associated fungal community and the viruses infecting them, is the first reporting the presence of marine mycoviruses: in the past, only two articles described a dsDNA virus in pseudo-fungal marine microorganisms ( Dawe andKuhn, 1983 andTakao et al, 2007), which modern taxonomy has shown to belong to the kingdom Protista. A very recent survey of various oceanic water samples distributed in all the major oceans (the circumglobal Tara Oceans expedition) provided a comprehensive overview of the Eukaryotic plankton diversity and of the global ocean microbiome .…”

Section: Discussionmentioning

confidence: 99%

Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi

Nerva¹,

et al. 2016

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Highlights• First report of mycoviruses isolated from fungi from marine environment.• Survey of mycoviruses using multiple approaches for both DNA and RNA genomes.• RNAseq analysis is superior to sRNA for de novo assembly of mycoviruses.• Twelve new virus species were characterized molecularly.• Expression of a viral RNA from an endogenized cDNA segment. AbstractThe number of reported mycoviruses is increasing exponentially due to the current ability to detect mycoviruses using next-generation sequencing (NGS) approaches, with a large number of viral genomes built in-silico using data from fungal transcriptome projects. We decided to screen a collection of fungi originating from a specific marine environment (associated with the seagrass Posidonia oceanica) for the presence of mycoviruses: our findings reveal a wealth of diversity among these symbionts and this complexity will require further studies to address their specific role in this ecological niche. In specific, we identified twelve new virus species belonging to nine distinct lineages: they are members of megabirnavirus, totivirus, chrysovirus, partitivirus and five still undefined clades. We showed evidence of an endogenized virus ORF, and evidence of accumulation of dsRNA from metaviridae retroviral elements. We applied different techniques for detecting the presence of mycoviruses including (i) dsRNA extraction and cDNA cloning, (ii) small and total RNA sequencing through NGS techniques, (iii) rolling circle amplification (RCA) and total DNA extraction analyses, (iv) virus purifications and electron microscopy. We tried also to critically evaluate the intrinsic value and limitations of each of these techniques. Based on the samples we could compare directly, RNAseq analysis is superior to sRNA for de novo assembly of mycoviruses. To our knowledge this is the first report on the virome of fungi isolated from marine environment.

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Rhizidiovirus

Ghabrial¹

2011

The Springer Index of Viruses

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Virus-like particles in the aquatic fungus, Rhizidiomyces

Cited by 11 publications

References 26 publications

Ultrastructural studies on a chlorella virus from Germany

Ultrastructural studies on a chlorella virus from Germany

Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi

Rhizidiovirus

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