RNA silencing of South African cassava mosaic virus in transgenic cassava expressing AC1/AC4 hp- RNA induces tolerance

Walsh, Helen; Vanderschuren, Hervé; Taylor, Sarah H.; Rey, M. E. C.

doi:10.1016/j.btre.2019.e00383

Cited by 10 publications

(5 citation statements)

References 103 publications

(148 reference statements)

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“…Tomato plants expressing hpRNA corresponding to the TYLCV-Rep coding sequence produce 21 and 22 nt siRNAs and are resistant to TYLCV [153]. Similarly, Cassava transgenics containing hpRNA homologous to the overlapping region of South African cassava mosaic virus (SACMV)-Rep and the AC1/AC4 were tolerant to cassava mosaic disease [154]. In another study, three distinct intron hpRNA constructs comprising sequences of AC2, AC4, and a fusion of AC2 and AC4 (AC2 + AC4) of seven begomoviruses were used.…”

Section: Sirna Shieldmentioning

confidence: 99%

When an Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants

Rahman,

Sanan-Mishra

2024

Agriculture

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Viruses are silent enemies that intrude and take control of the plant cell’s machinery for their own multiplication. Infection by viruses and the resulting damage is still a major challenge in the agriculture sector. Plants have the capability to fight back, but the ability of viruses to mutate at a fast rate helps them to evade the host’s response. Therefore, classical approaches for introgressing resistance genes by breeding have obtained limited success in counteracting the virus menace. Genetic modification (GM)-based strategies have been successful in engineering artificial resistance in plants. Several different approaches based on pathogen-derived resistance, antisense constructs, hairpin RNAs, double-stranded RNA, etc., have been used to enhance plants’ resistance to viruses. Recently, genome editing (GE) strategies mainly involving the CRISPR/Cas-mediated modifications are being used for virus control. In this review, we discuss the developments and advancements in GM- and GE-based methods for tackling viral infection in plants.

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Section: Sirna Shieldmentioning

confidence: 99%

When an Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants

Rahman,

Sanan-Mishra

2024

Agriculture

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“…Transgenic experiments for improving agronomic traits of crops have been mostly successful in plant species for which transformation has been perfected. Cassava with enormous bioenergy potential are recalcitrant to genetic transformation as the protocols optimized are cultivar dependent (Elegba et al, 2021;Liu et al, 2011;Nyaboga et al, 2013Nyaboga et al, , 2015Ojola et al, 2018;Walsh et al, 2019). Plant beneficial endophytes offer environment-friendly methods for improving plant biomass which is advantageous for bioenergy production.…”

Section: Enhancing Biomass and Bioconversion Of Cassava For Energy By...mentioning

confidence: 99%

Cassava (Manihot esculenta) dual use for food and bioenergy: A review

Fathima

Sanitha

Tripathi

et al. 2022

Food and Energy Security

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Cassava is an important food crop with an average consumption of 50 kg/capita/year in Africa (FAO, 2018). The global cassava production stands at slightly over 11 tonnes per hectare. Over 63% of the 303 million tonnes produced globally in 2019 was from Africa (FAO, 2019). Despite being the leading region in cassava production, only 3000 tonnes of the produce are dedicated to non-food uses. The challenges associated with global fossil fuels

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“…Cassava mosaic disease (CMD), caused by a begomovirus, is a severe constraint to cassava production. A transgene-derived RNA hairpin, homologous to an overlapping region of the South African cassava mosaic virus (SACMV)-Rep and the VSR proteins (AC1/AC4), has been found to confer tolerance in the CMD-susceptible model cassava cultivar 60444 ( Walsh et al, 2019 ). All the hp-RNA constructs gave rise to secondary siRNAs following viral infection in the transgenics.…”

Section: Genetic Engineering For Virus Resistance With Secondary Sirnmentioning

confidence: 99%

Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology

et al. 2021

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The major components of RNA silencing include both transitive and systemic small RNAs, which are technically called secondary sRNAs. Double-stranded RNAs trigger systemic silencing pathways to negatively regulate gene expression. The secondary siRNAs generated as a result of transitive silencing also play a substantial role in gene silencing especially in antiviral defense. In this review, we first describe the discovery and pathways of transitivity with emphasis on RNA-dependent RNA polymerases followed by description on the short range and systemic spread of silencing. We also provide an in-depth view on the various size classes of secondary siRNAs and their different roles in RNA silencing including their categorization based on their biogenesis. The other regulatory roles of secondary siRNAs in transgene silencing, virus-induced gene silencing, transitivity, and trans-species transfer have also been detailed. The possible implications and applications of systemic silencing and the different gene silencing tools developed are also described. The details on mobility and roles of secondary siRNAs derived from viral genome in plant defense against the respective viruses are presented. This entails the description of other compatible plant–virus interactions and the corresponding small RNAs that determine recovery from disease symptoms, exclusion of viruses from shoot meristems, and natural resistance. The last section presents an overview on the usefulness of RNA silencing for management of viral infections in crop plants.

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RNA silencing of South African cassava mosaic virus in transgenic cassava expressing AC1/AC4 hp- RNA induces tolerance

Cited by 10 publications

References 103 publications

When an Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants

When an Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants

Cassava (Manihot esculenta) dual use for food and bioenergy: A review

Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology

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