RNA sequencing as a powerful tool in searching for genes influencing health and performance traits of horses

Abstract

RNA sequencing (RNA-seq) by next-generation technology is a powerful tool which creates new possibilities in whole-transcriptome analysis. In recent years, with the use of the RNA-seq method, several studies expanded transcriptional gene profiles to understand interactions between genotype and phenotype, supremely contributing to the field of equine biology. To date, in horses, massive parallel sequencing of cDNA has been successfully used to identify and quantify mRNA levels in several normal tissues, as well as to annotate genes. Moreover, the RNA-seq method has been applied to identify the genetic basis of several diseases or to investigate organism adaptation processes to the training conditions. The use of the RNA-seq approach has also confirmed that horses can be useful as a large animal model for human disease, especially in the field of immune response. The presented review summarizes the achievements of profiling gene expression in horses (Equus caballus).

Use of RNA‐seq to determine variation in canine cytochrome P450 mRNA expression between blood, liver, lung, kidney and duodenum in healthy beagles

“…RNA‐seq has been used in physiologic studies to characterize the equine transcriptome (Stefaniuk & Ropka‐Molik, ) and alterations in the transcriptome of Holstein milk over a 250‐day period (Wickramasinghe, Rincon, Islas‐Trejo, & Medrano, ). Pathological conditions such as changes in the immune response of sheep to Fasciola hepatica (Rojas et al., ) and pathway analysis of canine lymphoma (Mooney et al., ) highlight the use of RNA‐seq in monitoring alterations in the transcriptome in response to disease states.…”

Research Progress in Genetic Control of Reproductive Performance in Chicken by High-Throughput Sequencing Technology

“…In recent years, transcriptome sequencing analysis technology has been extensively applied in studying the molecular regulation mechanisms related with growth, metabolism, disease resistance, and so on quantitative traits in agricultural animals [31][32][33][34][35]. In the field of chicken reproductive performance regulation-related research, Ayers et al studied the mechanism of sex differentiation in chicken using the RNA-seq technology and found that most of the differentially expressed genes in different genders come from autosomal rather than sex-chromosome linkage, many of which are novel genes for sexual differentiation, such as CAPN5, GPR56, and FGFR3 [36].…”

Development and validation of KASP markers for the milk traits genes in Kazakh horse

“…Recent advances in next-generation sequencing (NGS) technology have provided researchers with unprecedented tools to increase the discovery of genetic variation associated with traits of economic importance in animals (Petersen et al 2013;Crisà et al 2016;Stefaniuk et al 2016;Purfield et al 2017). But this method was rarely reported to be used to study the milk traits of horse.…”