RNA-Seq Revealed a Circular RNA-microRNA-mRNA Regulatory Network in Hantaan Virus Infection

Abstract

In this work, HUVECs were mock infected or infected with HTNV for 3 days. Total RNA of the cells were analyzed by RNA-seq and obtained circRNA, miRNA, mRNA library. Differentially expressed (DE) RNAs were identified and subjected to GO analysis, KEGG analysis and ceRNA network construction. Then 8 DE circRNAs, 8 DE mRNAs, 6 DE miRNAs were verified by RT-qPCR. Besides, mRNAs (CMPK2, PARP10, GBP1, and RIG-I), circRNAs (circ_0000479), miRNAs (miR-149-5p, miR-411-3p, and miR-330-5p) in the ceRNA network were found effective to inhibit or promote virus replication. And the circ_0000479-miR-149-5p-RIG-I ceRNA axis was verified in HTNV infection.

Comprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of Trichosporon asahii infection

“…Several recent studies have reported circRNA expression profiles and ceRNA networks in viral and bacterial infectious diseases [ 25 – 28 ]. Nevertheless, the expression profiles and potential roles of circRNAs in fungal infection have not been explored.…”

Conprehensive Analysis of circRNA-microRNA-mRNA Network Revealed Novel Regulatory Mechanism in Trichosposon Asahii Infection

“…Recently, several studies have reported the cricRNA expression pro les and ceRNA network in viral and bacteria infection diseases [24][25][26][27]. Nevertheless, the expression pro les and potential roles of circRNAs in fungi infections have not been explored.…”

The role of circular RNAs in viral infection and related diseases

“…HTNV infection in humans causes high mortality of hemorrhagic fever with renal syndrome (HFRS). Circ_0000479 expression in HUVEC has been found to increase after HTNV infection, which in turn to inhibit viral replication by sponging miR-149−5p and promoting RIG-I expression ( Lu et al, 2020 ). The expression of hsa_circ_0004812 in liver cancer tissues of CHB patients is significantly up-regulated.…”

“…Studies have shown that the virus-generated circRNAs or differentially expressed host circRNAs can be used as candidate biomarkers for viral infection ( Wang et al, 2018 ; Yu et al, 2020 ; Zhao et al, 2019a ). Meanwhile, circRNAs are involved in regulating antiviral immune response ( Chen et al, 2017 ; Li et al, 2017 ), viral replication ( Lu et al, 2020 ; Sekiba et al, 2018 ; Tagawa et al, 2018 ; Yu et al, 2019 ; Zhang and Wang, 2020 ), and pathogenesis of infectious diseases ( Zhang et al, 2019 ; Zhao et al, 2019a , b ). In this review, we summarized the current understanding of circRNAs, mainly focused on the circRNAs in the regulation of viral infections.…”