RNA-seq Profiling Showed Divergent Carbohydrate-Active Enzymes (CAZymes) Expression Patterns in Lentinula edodes at Brown Film Formation Stage Under Blue Light Induction

Abstract

Lentinula edodes (shiitake mushroom) is one of the most important edible mushrooms worldwide. The L. edodes cultivation cycle includes a unique developing stage called brown film formation that directly affects the development of primordium and the quality of fruiting body. Brown film formation is induced by light, especially blue light. To promote our understanding of the role of blue light in brown film formation mechanisms of L. edodes, we used RNA-seq and compared the transcriptomes of L. edodes grown under blue light and in dark, and validated the expression profiles using qRT-PCR. Blue light stimulated the formation of brown film and increased the content of polysaccharides in L. edodes. Blue light also promoted L. edodes to absorb more polysaccharides by enhancing the activities of enzymes. Among the 730 differentially expressed genes (DEGs), 433 genes were up-regulated and 297 were down-regulated. Most of the DEGs were in the oxidoreductase activity group. Pentose and glucuronic acid conversion and starch and sucrose metabolism were the most important pathways in the formation of brown film. A total of 79 genes of DEGs were identified as genes encoding carbohydrate-active enzymes (CAZymes). Fifty-one of the CAZymes genes were up-regulated, suggesting that CAZymes play important roles in brown film formation to provide sufficient nutrition for L. edodes. The results will facilitate future functional investigations of the genes involved in the developmental control of L. edodes.

Transcriptional Dynamics of Genes Purportedly Involved in the Control of Meiosis, Carbohydrate, and Secondary Metabolism during Sporulation in Ganoderma lucidum

“…Transcriptome analysis of different developmental stages of peridioles identified transcription factors (TFs) that may play a role in this regulation [ 16 ]. Previous studies on comparative transcriptome analysis have identified candidate genes involved in mycelium browning [ 17 , 18 ] and revealed a high rate of divergence in developmental gene expression as well as several genes with conserved expression patterns [ 19 , 20 ]. These studies indicate that RNA-seq provides an effective way to explore the underlying regulatory networks of favorable traits in fungi.…”

TMT‐MS/MS proteomic analysis of the carbohydrate‐active enzymes in the fruiting body of Pleurotus tuoliensis during storage

“…At present, several studies on the use of omics to study CAZymes have been published. For example, Huang et al ., 15 in their study used RNA‐seq to identify 52 upregulated CAZymes in Lentinula edodes , and found that these upregulated CAZymes may play important roles in brown film formation to provide sufficient nutrition for L. edodes . With metabolic and proteomic analysis, 139 CAZymes and 10 browning‐related enzymes were found to be involved in the texture and color regulation of morel fruiting bodies 14 .…”

Enhancement of polysaccharides production using microparticle enhanced technology by Paraisaria dubia

“…In the bacterial polysaccharide synthesis pathway, sugar nucleotides are the most basic glycosyl donor unit, and their efficient supply is the key to achieving the mass synthesis of polysaccharides [ 27 , 28 ]. Monosaccharides, mostly glucose in this study, need to undergo a series of reactions after they enter the cell to be converted into the glycosyl donor unit [ 29 ].…”