RNA-Seq profiling of circular RNA in human lung adenocarcinoma and squamous cell carcinoma

Wang, Chengdi; Tan, Shuangyan; Liu, Wenrong; Lei, Qian; Qiao, Wenliang; Wu, Yangping; Liu, Xiaoqi; Cheng, Wei; Wei, Yuquan; Peng, Yong; Liu, Weimin

doi:10.1186/s12943-019-1061-8

Cited by 137 publications

(89 citation statements)

References 9 publications

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“…The present study suggested that circEPB41L2 was obviously decreased in CRC tumors, which might make it a potential tumor suppressor. circEPB41L2 was also found to be downregulated in lung adenocarcinoma and squamous cell carcinoma, supporting its status as a possible useful biomarker and tumor suppressor in both conditions [24]. Here, our additional experiments further confirmed the tumor-suppressive role of circEPB41L2 and revealed the function of circEPB41L2, which is exerted by sponging miR-21.…”

Section: Discussionsupporting

confidence: 74%

Novel circular RNA hsa_circ_0077837 suppresses colorectal cancer cell proliferation by regulating the microRNA 21/phosphatase and tensin homolog axis

Sheng

Dong

Zhang

et al. 2020

Preprint

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Background: Circular RNA (circRNA) is a novel noncoding RNA (ncRNA) that assumes critical roles in tumor occurrence and progression. circRNA functions in many tumors as a kind of microRNA (mRNA) “sponge.” The present study sought to investigate the part played by circRNA in colorectal cancer (CRC) progression and to elucidate the relevant action mechanism(s). Methods: We detected lower hsa_circ_0077837 (circEPB41L2) expression in CRC by data analysis based on previous studies. We demonstrated the antitumor part of circEPB41L2 in CRC cells via a group of biological experiments. We subsequently took advantage of pull-down and dual-luciferase reporter assays to recognize circEPB41L2 downstream microRNA 21 (miR-21). Western blotting was conducted to confirm that the circEPB41L2–miR-21–phosphatase and tensin homolog (Pten)/AKT axis is responsible for CRC tumor growth suppression. The antitumor part of circEPB41L2 in vivo was eventually demonstrated by HCT116 xenograft model. Results: We found that circEPB41L2 overexpression inhibited the proliferation of CRC cells. Further, the miR-21 phenocopy suppressed circNRIP1 overexpression, while its overexpression also inhibited the cancer suppressive function of circEPB41L2. circEPB41L2 was confirmed to be able to inhibit tumor growth by enhancing Pten expression. Finally, the antitumor part of circEPB41L2 was confirmed. Conclusions: We demonstrated that circEPB41L2 sponges miR-21 to increase the Pten expression level and consequently serve as a cancer suppressor in CRC.

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Section: Discussionsupporting

confidence: 74%

Novel circular RNA hsa_circ_0077837 suppresses colorectal cancer cell proliferation by regulating the microRNA 21/phosphatase and tensin homolog axis

Sheng

Dong

Zhang

et al. 2020

Preprint

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“…There are some reports suggesting that the abnormal expression of circRNAs could be found in plasma from cancer patients ( 61 – 63 ). Furthermore, Wang et al demonstrated that circRNAs had the potential role to distinguish LUAD and LUSQ, indicating that circRNAs may play a different role in the initiation and development of LUAD and LUSQ ( 64 ). In recent years, owing to the popularization of CT as a diagnostic method of early lung cancer, the mortality rate of lung cancer patients has been significantly reduced ( 65 ).…”

Section: Discussionmentioning

confidence: 99%

Circular RNA Signature in Lung Adenocarcinoma: A MiOncoCirc Database-Based Study and Literature Review

Zhao

Liu

et al. 2020

Front. Oncol.

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Circular RNAs (circRNAs) are a class of endogenous non-coding RNAs (ncRNAs) with a structure of covalently closed continuous loops, which can regulate gene expression by acting as a microRNA sponge or through other mechanisms. Recent studies have identified that the expression of candidate circRNAs are dysregulated in various tumors and hence are considered as promising diagnostic or therapeutic targets across cancer types. However, the expression and function of circRNAs in lung adenocarcinoma (LUAD) remains unclear. In this article, we investigated the expression of circRNAs in LUAD via MiOncoCirc, which is the first and comprehensive database characterizing circRNAs across >2,000 cancer samples using an exome capture RNA sequencing. We identified seven abnormally expressed circRNAs in LUAD, including circCDR1-AS, circHIPK3, circFNDC3B, circPCMTD1, circRHOBTB3, circFAM13B, and circMAN1A2, as well as conducted a literature review about the function and features of these circRNAs. Previous studies have demonstrated that circCDR1-AS, circMAN1A2, and circHIPK3 were upregulated and significantly correlated with a poor survival, or promoted the tumor progression in lung cancer, whereas other circRNAs have not been fully explored. Besides, we reviewed all the publications regarding circRNAs and LUAD, and noticed that the dysregulation of these circRNAs impacts the development of LUAD through a variety of regulatory mechanisms. In conclusion, the underlying mechanisms of aberrant expression and functions of circRNAs in LUAD are worthy of being further investigated.

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“…The highly expressed hsa_circ_0014130 in NSCLC tissues was indicated lymph node metastasis and dismal TNM stage. Wang et al 11 discovered the important diagnostic potential of circRNA in NSCLC. They noticed that hsa_circ_0077837 and hsa_circ_0001821 might be potential biomarkers for lung adenocarcinoma and squamous cell carcinoma.…”

Section: Introductionmentioning

confidence: 99%

<p>CircRNA hsa_circ_0087862 Acts as an Oncogene in Non-Small Cell Lung Cancer by Targeting miR-1253/RAB3D Axis</p>

Wan

Xiong

et al. 2020

OTT

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Purpose: Circular RNAs (circRNAs) have been found to regulate several human tumors. The present study was to explore the mechanism of hsa_circ_0087862 in regulating nonsmall cell lung cancer (NSCLC). Methods: Totally 102 NSCLC cases were enrolled. NCI-H1359 and A549 cells were transfected. Cells viability, apoptosis, migration and invasion were determined by CCK-8 assay, flow cytometry, scratch test and transwell experiment, respectively. Luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay were performed. Xenograft tumor experiments were performed using nude mice. hsa_circ_0087862, miR-1253 and RAB3D expression in tissues/cells were detected by qRT-PCR. RAB3D and Ki67 protein expressions in cells/tissues were researched by Western blot and immunohistochemistry. Apoptosis of xenograft tumor tissue cells was detected using Tunel assay. Results: hsa_circ_0087862 was significantly up-regulated in NSCLC patients, which was associated with poor prognosis (P < 0.05). hsa_circ_0087862 down-regulation prominently weakened NSCLC cells viability, migration, invasion and enhanced apoptosis (P < 0.01). hsa_circ_0087862 overexpression exhibited the opposite results in NSCLC cells. miR-1253 was sponged by hsa_circ_0087862. miR-1253 expression in NSCLC tissues was negatively correlated with hsa_circ_0087862 (P < 0.001). RAB3D expression in NSCLC was directly inhibited by miR-1253. miR-1253 down-regulation or RAB3D overexpression dramatically reversed NSCLC cells phenotype induced by hsa_circ_0087862 down-regulation. hsa_circ_0087862 down-regulation markedly inhibited tumor growth in vivo (P < 0.01). In xenograft tumor tissues, hsa_circ_0087862 down-regulation obviously decreased expression of RAB3D, Ki67 and increased apoptosis. Conclusion: hsa_circ_0087862 acted as an oncogene in NSCLC by targeting miR-1253/ RAB3D.

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RNA-Seq profiling of circular RNA in human lung adenocarcinoma and squamous cell carcinoma

Cited by 137 publications

References 9 publications

Novel circular RNA hsa_circ_0077837 suppresses colorectal cancer cell proliferation by regulating the microRNA 21/phosphatase and tensin homolog axis

Novel circular RNA hsa_circ_0077837 suppresses colorectal cancer cell proliferation by regulating the microRNA 21/phosphatase and tensin homolog axis

Circular RNA Signature in Lung Adenocarcinoma: A MiOncoCirc Database-Based Study and Literature Review

<p>CircRNA hsa_circ_0087862 Acts as an Oncogene in Non-Small Cell Lung Cancer by Targeting miR-1253/RAB3D Axis</p>

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