<p>CircRNA hsa_circ_0087862 Acts as an Oncogene in Non-Small Cell Lung Cancer by Targeting miR-1253/RAB3D Axis</p>

Abstract

Purpose: Circular RNAs (circRNAs) have been found to regulate several human tumors. The present study was to explore the mechanism of hsa_circ_0087862 in regulating nonsmall cell lung cancer (NSCLC). Methods: Totally 102 NSCLC cases were enrolled. NCI-H1359 and A549 cells were transfected. Cells viability, apoptosis, migration and invasion were determined by CCK-8 assay, flow cytometry, scratch test and transwell experiment, respectively. Luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay were performed. Xenograft tumor experiments were performed using nude mice. hsa_circ_0087862, miR-1253 and RAB3D expression in tissues/cells were detected by qRT-PCR. RAB3D and Ki67 protein expressions in cells/tissues were researched by Western blot and immunohistochemistry. Apoptosis of xenograft tumor tissue cells was detected using Tunel assay. Results: hsa_circ_0087862 was significantly up-regulated in NSCLC patients, which was associated with poor prognosis (P < 0.05). hsa_circ_0087862 down-regulation prominently weakened NSCLC cells viability, migration, invasion and enhanced apoptosis (P < 0.01). hsa_circ_0087862 overexpression exhibited the opposite results in NSCLC cells. miR-1253 was sponged by hsa_circ_0087862. miR-1253 expression in NSCLC tissues was negatively correlated with hsa_circ_0087862 (P < 0.001). RAB3D expression in NSCLC was directly inhibited by miR-1253. miR-1253 down-regulation or RAB3D overexpression dramatically reversed NSCLC cells phenotype induced by hsa_circ_0087862 down-regulation. hsa_circ_0087862 down-regulation markedly inhibited tumor growth in vivo (P < 0.01). In xenograft tumor tissues, hsa_circ_0087862 down-regulation obviously decreased expression of RAB3D, Ki67 and increased apoptosis. Conclusion: hsa_circ_0087862 acted as an oncogene in NSCLC by targeting miR-1253/ RAB3D.

Circ_cse1l Inhibits Colorectal Cancer Proliferation by Binding to eIF4A3

“…CircRNA is a special type of endogenous non-coding RNA (ncRNA) [ 22 ]. circRNA is differentially expressed in various types of malignant tumor, including CRC, with its level of expression being closely related to clinical stage and prognosis [ 23 , 24 ].…”

Cisplatin and phenanthriplatin modulate long-noncoding RNA expression in A549 and IMR90 cells revealing regulation of microRNAs, Wnt/β-catenin and TGF-β signaling

“…Also, we found that cisplatin downregulated the lncRNA, lnc-PGPEP1L-3, which is predicted to sponge miR-25-5p, a miRNA with high sequence homology to lnc-MRPL39-10, a lncRNA upregulated by phenanthriplatin (Table 2; Supplementary Table S1). These miRNAs are associated with Wnt/β-catenin and TGF-β signaling, e.g., miR-25-5p, -30a-3p, -138-5p, -149-3p, -185-5p, -378j, -608, -650, -708-5p, -1253, -1254 and − 4516 (Table 2) [23][24][25][26][27][28][29][30][31][32][33][34][35]. We also used microarray analysis to identify the most up-and downregulated lncRNAs in IMR90 cells and found a set of lncRNAs up-and downregulated by cisplatin or phenanthriplatin compared to control treatment (Table 3).…”

Cisplatin and phenanthriplatin modulate long-noncoding RNA expression in A549 and IMR90 cells revealing regulation of microRNAs, Wnt/β-catenin and TGF-β signaling

“…Also, we found that cisplatin downregulated the lncRNA, lnc-PGPEP1L-3, which is predicted to sponge miR-25-5p, a miRNA with high sequence homology to lnc-MRPL39-10, a lncRNA upregulated by phenanthriplatin (Table 3; Supplementary Table S1). These miRNAs are associated with Wnt/β-catenin and TGF-β signaling, e.g., miR-25-5p, -30a-3p, -138-5p, -149-3p, -185-5p, -378j, -608, -650, -708-5p, -1253, -1254 and -4516 (Table 3) [23][24][25][26][27][28][29][30][31][32][33][34][35] .…”