RNA-Seq analysis reveals genes associated with resistance to Taura syndrome virus (TSV) in the Pacific white shrimp Litopenaeus vannamei

Use of RNA-seq in Aquaculture Research

“…454 pyrosequencing-based RNA-Seq results revealed that apoptosis, mitogen-activated protein kinase signaling, toll-like receptor signaling, Wnt signaling and antigen processing and presentation pathways functioned importantly in defending against White Spot Syndrome Virus in white shrimp [10]. Similar studies were conducted in Vibrio harveyi challenged Asian seabass [8] and Japanese sea bass [17], white shrimp with Taura syndrome virus [20], and Chinese shrimp challenged with White Spot Syndrome Virus [21]. In zebrafish, RNAseq was utilized to investigate the expression patterns of immunizationrelated genes immunized with vaccines against E. tarda [19], as well as in European sea bass with vaccines against V. anguillarum [18].…”

Transcriptomic response to low salinity stress in gills of the Pacific white shrimp, Litopenaeus vannamei

“…Li et al, 2014;L. Li et al, 2014;Lv et al, 2013;Sookruksawong et al, 2013). It is fast and highly sensitive.…”

RNA-seq as a powerful tool for penaeid shrimp genetic progress

“…SRA databank, per instance, presents only 28 deposits of data generated by NGS in what regards the species L. vannamei , L. stylirostris, and Penaeus monodon (). In literature, it has been found next-gen data only for P. monodon (Baranski et al, 2014) Fenneropenaeus chinensis (Li et al, 2013) and L. vannamei (Li et al, 2012; Guo et al, 2013; Sookruksawong et al, 2013; Xue et al, 2013; Zeng et al, 2013; Yu et al, 2014). Mostly, the research in this field has been covering the identification of genes connected to immunity, mainly concerning the white spot syndrome virus (WSSV) and the taura syndrome virus (TSV; Li et al, 2013; Sookruksawong et al, 2013; Xue et al, 2013; Zeng et al, 2013; Baranski et al, 2014).…”

“…However, RNA-seq approaches are still incipient in shrimp ( Table 1 ; Li et al, 2012, 2013; Guo et al, 2013; Sookruksawong et al, 2013; Xue et al, 2013; Zeng et al, 2013; Baranski et al, 2014; Yu et al, 2014). Therefore, the method herein has emerged as a new possibility for the transcriptome complexity analyses in face of varied production and/or experimental conditions.…”