RNA-seq analysis revealed key genes associated with salt tolerance in rapeseed germination through carbohydrate metabolism, hormone, and MAPK signaling pathways

Mohamed, Ibrahim A. A.; Shalby, Nesma; El-Badri, Ali Mahmoud; Batool, Maria; Wang, Chunyun; Wang, Zongkai; Salah, Akram; Rady, Mostafa M.; Kuai, Jie; Wang, Bo; Zhou, Guangsheng

doi:10.1016/j.indcrop.2021.114262

Cited by 43 publications

(16 citation statements)

References 115 publications

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“…Huayouza 62 is an elite hybrid rapeseed variety, successfully promoted and planted in China's saline-alkali land due to its relatively strong salt tolerance. Zhongshuang 11 is an elite conventional rapeseed variety; however, it is very sensitive to salt stress [29]. Our results showed a significant difference between H62 and ZS11 in salt tolerance at the early-seedling stage (Figure 1).…”

Section: Discussionmentioning

confidence: 63%

“…However, the molecular mechanism of salt tolerance in H62 rapeseed is still unclear. Zhongshuang 11 (ZS11) is a conventional rapeseed variety, which is sensitive to salt stress [29]. In this study, the salt-tolerant variety (H62) and salt-sensitive variety (ZS11) were selected, and RNA-seq and metabolomics analysis methods were employed to analyze key salt stress response genes and metabolites in rapeseed under salt stress at the germination stage.…”

Section: Introductionmentioning

confidence: 99%

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Combined Transcriptomics and Metabolomics Analysis Reveals the Molecular Mechanism of Salt Tolerance of Huayouza 62, an Elite Cultivar in Rapeseed (Brassica napus L.)

Wan

Qian

Zhang

et al. 2022

IJMS

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Soil salinity is one of the most significant abiotic stresses affecting crop yield around the world. To explore the molecular mechanism of salt tolerance in rapeseed (Brassica napus L.), the transcriptome analysis and metabolomics analysis were used to dissect the differentially expressed genes and metabolites in two rapeseed varieties with significant differences in salt tolerance; one is an elite rapeseed cultivar, Huayouza 62. A total of 103 key differentially expressed metabolites (DEMs) and 53 key differentials expressed genes (DEGs) that might be related to salt stress were identified through metabolomics and transcriptomics analysis. GO and KEGG analysis revealed that the DEGs were mainly involved in ion transport, reactive oxygen scavenging, osmotic regulation substance synthesis, and macromolecular protein synthesis. The DEMs were involved in TCA cycle, proline metabolism, inositol metabolism, carbohydrate metabolic processes, and oxidation-reduction processes. In addition, overexpression of BnLTP3, which was one of the key DEGs, could increase tolerance to salt stress in Arabidopsis plants. This study reveals that the regulation mechanism of salt tolerance in rapeseed at the transcriptome and metabolism level and provides abundant data for further in-depth identification of essential salt tolerance genes.

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Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Combined Transcriptomics and Metabolomics Analysis Reveals the Molecular Mechanism of Salt Tolerance of Huayouza 62, an Elite Cultivar in Rapeseed (Brassica napus L.)

Wan

Qian

Zhang

et al. 2022

IJMS

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“…Salt stress is a severe hazard that adversely affects all stages of plant growth, such as inhibiting seed germination, slowing plant development, decreasing biomass yield, and decreasing crop yield [ 1 , 29 ]. Therefore, it is a great advantage to breed salt-tolerant rapeseed cultivars.…”

Section: Discussionmentioning

confidence: 99%

“…The expound on the alkaline salt tolerance mechanism is beneficial to the breeding of new resistant varieties. Although many rapeseed cultivars with high diversity in salt resistance have been reported [ 29 ], the details about genes and mechanisms of tolerance to alkalinity are largely unknown in rapeseed. Transcriptomic analysis can provide mRNA expression of genes at high throughput and is widely applied to screen candidate genes involved in stress responses [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…Although many rapeseed cultivars with high diversity in salt resistance have been reported [ 29 ], the details about genes and mechanisms of tolerance to alkalinity are largely unknown in rapeseed. Transcriptomic analysis can provide mRNA expression of genes at high throughput and is widely applied to screen candidate genes involved in stress responses [ 29 , 30 ]. In the present study, an RNA-seq was performed on alkaline-tolerant and -sensitive rapeseed cultivars to screen alkaline-salt-tolerance-related genes, which will lay a solid foundation for the breeding and mechanism of alkaline salt tolerance in B. napus .…”

Section: Introductionmentioning

confidence: 99%

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Identification of Alkaline Salt Tolerance Genes in Brassica napus L. by Transcriptome Analysis

Tao

Zhu

et al. 2022

Genes

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Soil salt alkalization is one major abiotic factor reducing the productivity of crops, including rapeseed, an indispensable oil crop and vegetable. The mechanism studies of alkali salt tolerance can help breed highly resistant varieties. In the current study, rapeseed (B. napus) line 2205 exhibited more tolerance to alkaline salt than line 1423 did. In line 2205, the lesser plasma membrane damage index, the accumulated osmotic solute, and higher antioxidant enzyme activities contributed to alkaline tolerance. A more integrated mesophyll-cell structure was revealed under alkali salt stress by ultrastructure observation in line 2205, which also implied a lesser injury. Transcriptome analysis showed that more genes responded to alkaline salt in line 2205. The expression of specific-response genes in line 1423 was lower than in line 2205. However, most of the specific-response genes in line 2205 had higher expression, which was mainly enriched in carbohydrate metabolism, photosynthetic processes, ROS regulating, and response to salt stress. It can be seen that the tolerance to alkaline salt is attributed to the high expression of some genes in these pathways. Based on these, twelve cross-differentially expressed genes were proposed as candidates. They provide clues for further analysis of the resistance mechanism of rapeseed.

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Role of Phytomelatonin in Promoting Ion Homeostasis During Salt Stress

El-Badri,

Batool,

Mohamed

et al. 2023

Plant in Challenging Environments

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RNA-seq analysis revealed key genes associated with salt tolerance in rapeseed germination through carbohydrate metabolism, hormone, and MAPK signaling pathways

Cited by 43 publications

References 115 publications

Combined Transcriptomics and Metabolomics Analysis Reveals the Molecular Mechanism of Salt Tolerance of Huayouza 62, an Elite Cultivar in Rapeseed (Brassica napus L.)

Combined Transcriptomics and Metabolomics Analysis Reveals the Molecular Mechanism of Salt Tolerance of Huayouza 62, an Elite Cultivar in Rapeseed (Brassica napus L.)

Identification of Alkaline Salt Tolerance Genes in Brassica napus L. by Transcriptome Analysis

Role of Phytomelatonin in Promoting Ion Homeostasis During Salt Stress

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