Identification of Alkaline Salt Tolerance Genes in Brassica napus L. by Transcriptome Analysis

Abstract

Soil salt alkalization is one major abiotic factor reducing the productivity of crops, including rapeseed, an indispensable oil crop and vegetable. The mechanism studies of alkali salt tolerance can help breed highly resistant varieties. In the current study, rapeseed (B. napus) line 2205 exhibited more tolerance to alkaline salt than line 1423 did. In line 2205, the lesser plasma membrane damage index, the accumulated osmotic solute, and higher antioxidant enzyme activities contributed to alkaline tolerance. A more integrated mesophyll-cell structure was revealed under alkali salt stress by ultrastructure observation in line 2205, which also implied a lesser injury. Transcriptome analysis showed that more genes responded to alkaline salt in line 2205. The expression of specific-response genes in line 1423 was lower than in line 2205. However, most of the specific-response genes in line 2205 had higher expression, which was mainly enriched in carbohydrate metabolism, photosynthetic processes, ROS regulating, and response to salt stress. It can be seen that the tolerance to alkaline salt is attributed to the high expression of some genes in these pathways. Based on these, twelve cross-differentially expressed genes were proposed as candidates. They provide clues for further analysis of the resistance mechanism of rapeseed.

Integrated metabolomic and transcriptomic analysis reveals the role of phenylpropanoid biosynthesis pathway in tomato roots during salt stress

“…Currently, various omics techniques have become an effective strategy to better understand the response mechanisms to environmental stresses such as salt stress ( Dai et al., 2022 ). In recent years, there have been many reports on the study of salt tolerance in plants through transcriptomic approaches ( Chen et al., 2022a ; Ge et al., 2022 ; Wang et al., 2022a ; Xu et al., 2022 ). Recently, metabolomic studies on salt tolerance in plants have reported that increased accumulation of metabolites and related pathways that mainly regulate the antioxidant defense system in different Indian mustard ( Brassica juncea ) varieties under different periods of salt stress give the high-yielding variety CS60 an advantage over CS245-2-80-7 and may be the main tolerance mechanism for its resistance to salt stress ( Singh et al., 2022 ).…”

Integration of GWAS and RNA-Seq Analysis to Identify SNPs and Candidate Genes Associated with Alkali Stress Tolerance at the Germination Stage in Mung Bean

“…A transcriptomics analysis played a role in revealing the alkalinity adaptation strategies of lentils and in identifying the significantly upregulated differently expressed genes (DEGs) involved in abscisic acid (ABA) signaling and secondary metabolite synthesis [ 24 ]. Xu et al [ 25 ] identified 12 cross-differentially expressed genes in response to the alkali stress of rapeseed; the major genes that responded specifically in tolerant material were mainly enriched in carbohydrate metabolism, photosynthetic processes, ROS regulation, and the response to salt stress. In recent years, the combination of the GWAS and RNA-seq has proven to be reliable for identifying potential genes related to abiotic stress.…”