RNA profiling of fusarium head blight-resistant wheat addition lines containing the<i>Thinopyrum elongatum</i>chromosome 7E

Wang, J. R.; Wang, L.; Gulden, Sigrun; Rocheleau, Hélène; Balcerzak, Margaret; Hattori, Jiro; Cao, Wenguang; Han, Fangpu; Zheng, Youliang; Fedak, George; Ouellet, Thérèse

doi:10.1080/07060661003740512

Cited by 40 publications

(24 citation statements)

References 39 publications

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“…For global RNA profiling by microarray, plant total RNA from inoculated spikelet tissues (five spikes per biological replicate) was extracted using a guanidine isothiocyanate-cesium chloride method as described previously [31]. Two additional precipitations with ammonium acetate and ethanol were added at the end of the extraction to increase the quality of the RNAs.…”

Section: Rna Isolation and Cdna Synthesismentioning

confidence: 99%

Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1

Long

Balcerzak

Gulden

et al. 2015

Physiological and Molecular Plant Pathology

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Section: Rna Isolation and Cdna Synthesismentioning

confidence: 99%

Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1

Long

Balcerzak

Gulden

et al. 2015

Physiological and Molecular Plant Pathology

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“…For quantitative RT-PCR (qRT-PCR) analyses, RNA quality was monitored twice, with a nanometre and on agarose gel before and after being treated with an RNase-free DNase I (Qiagen, Mississauga, Canada). RNA clean up, cDNA synthesis, primer design, and qRT-PCR analyses were performed as described in Wang et al (2010). The primers used for qRT-PCR analyses are listed in Table 1.…”

Section: Gene Expression Analysesmentioning

confidence: 99%

Effect of salicylic acid on Fusarium graminearum, the major causal agent of fusarium head blight in wheat

et al. 2012

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“…Most frequently, b-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S or 26S ribosomal RNA (18S or 26S rRNA) and a-tubulin (TUB) had been validated as suitable internal control genes for qRT-PCR in the past years (Goidin et al 2001;Mitter et al 2009;Wang et al 2010). Unfortunately, many studies reported that those internal control genes were expressed irregularly and unsteadily in some experiments, questioning the concept of the ideal, universal internal control gene (Thellin et al 1999;Bustin 2002).…”

Section: Introductionmentioning

confidence: 96%

Genome-wide identification and evaluation of novel internal control genes for Q-PCR based transcript normalization in wheat

et al. 2010

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To accurately quantify gene expression using quantitative PCR amplification, it is vital that one or more ideal internal control genes are used to normalize the samples to be compared. Ideally, the expression level of those internal control genes should vary as little as possible between tissues, developmental stages and environmental conditions. In this study, 32 candidate genes for internal control were obtained from the analysis of nine independent experiments which included 333 Affymetrix GeneChip Wheat Genome arrays. Expression levels of the selected genes were then evaluated by quantitative real-time PCR with cDNA samples from different tissues, stages of development and environmental conditions. Finally, fifteen novel internal control genes were selected and their respective expression profiles were compared using NormFinder, geNorm, Pearson correlation coefficients and the twofold-change method. The novel internal control genes from this study were compared with thirteen traditional ones for their expression stability. It was observed that seven of the novel internal control genes were better than the traditional ones in expression stability under all the tested cDNA samples. Among the traditional internal control genes, the elongation factor 1-alpha exhibited strong expression stability, whereas the 18S rRNA, Alpha-tubulin, Actin and GAPDH genes had very poor expression stability in the range of wheat samples tested. Therefore, the use of the novel internal control genes for normalization should improve the accuracy and validity of gene expression analysis.

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RNA profiling of fusarium head blight-resistant wheat addition lines containing theThinopyrum elongatumchromosome 7E

Cited by 40 publications

References 39 publications

Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1

Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1

Effect of salicylic acid on Fusarium graminearum, the major causal agent of fusarium head blight in wheat

Genome-wide identification and evaluation of novel internal control genes for Q-PCR based transcript normalization in wheat

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