RNA-Loaded Chitosan Nanoparticles for Enhanced Growth, Immunostimulation and Disease Resistance in Fish

Ferosekhan, S.; Gupta, S. K.; Singh, Arvind; Rather, Mohd Ashraf; Kumari, Rakhi; Kothari, D.C.; Pal, A. K.; Jadhao, S. B.

doi:10.2174/1573413710666140115220300

Cited by 32 publications

(14 citation statements)

References 53 publications

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“…The sustained release of these nanoconjugated hormones results in decent reproductive output in fish. Chitosan nanoencapsulated RNA with particle size of 250 nm was developed and applied for increased growth performance, immunity and survival against bacterial challenge in fish (Ferosekhan, Gupta, Singh, Rather, Kumari, Kothari, Pal & Jadhao ). It is clear from above discussion that the nanoparticles developed in our study were much smaller in size, more stable with higher entrapment efficiency than the above reports.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization, tissue distribution of Follicle-Stimulating Hormone (FSH) beta subunit and effect of kisspeptin-10 on reproductive hormonal profile ofCatla catla(Hamilton, 1822)

et al. 2014

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Gonadotropin (GTH) hormones are glycoprotein which stimulates gonadal maturation in vertebrates. Follicle stimulating hormone is involved in initiation of gametogenesis and regulation of gonadal growth. FSHb has been cloned and characterized from the brain of Catla catla. The FSHb full-length of cDNA sequence of 523 bp comprised 3, 394 and 128 bp of 5 0 -UTR, open reading frame (ORF) 3 0 -UTR respectively. The coding region of C. catla FSHb encoded a peptide of 130 amino acids. Phylogenetic analysis of C. catla FSHb deduced amino acid sequence showed high similarity with Gobiocypris rarus followed by goldfish, Carassius auratus. The qPCR result shows that FSHb mRNA is mainly expressed in pituitary while moderate and low expression was observed in testis and ovary respectively. Chitosan-nanoconjugated kisspeptin-10 (CK-10) of particle size 125 nm, polydispersity index of 0.335 to 0.65 and zeta potential of À34.95 mV were synthesized and evaluated at against naked kisspeptin-10 for their reproductive hormonal profile. Treatment of fish with CK-10 showed controlled and sustained surge of the reproductive hormones (FSH & LH) with peak at 12 h. The hormone levels of naked kisspeptin-10 treated fish decline after 6 h. The sustained release of this CK-10 will help in reducing maturation age, synchronization of ovulation and spawning in fish. This is the first report on use of chitosannanoconjugated kisspeptin-10 (CK-10) for reproduction in fish.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization, tissue distribution of Follicle-Stimulating Hormone (FSH) beta subunit and effect of kisspeptin-10 on reproductive hormonal profile ofCatla catla(Hamilton, 1822)

et al. 2014

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“…Ferosekhan et al . () encapsulated dietary nucleotides with chitosan nanoparticles to recover low feed utilization efficiency in roho labeo ( Labeo rohita ) due to intestinal cell repulsion and its dependency on pH. They found that dietary chitosan nano‐sized RNA improved growth, immunity and disease resistance of fish compared with those fed bare RNA or chitosan alone.…”

Section: Chitin the Main Producer Of Chitosanmentioning

confidence: 99%

Effects of dietary chitosan supplementation on farmed fish; a review

Abdel‐Ghany

Salem

2019

Reviews in Aquaculture

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Chitosan is a cationic polysaccharide that is primarily derived from chitin, a natural polymer of N‐acetyl glucosamine mainly found in crustacean, insect exoskeletons and fungal cell walls, by exhaustive alkaline deacetylation to varying degrees. Chitosan has been found to have many favourable biological properties including biosafety, biodegradability and biocompatibility. These advantages make it a very promising material for diverse applications. For terrestrial animals, chitosan has been widely used as a feed additive because of its low side effects, enhancing the growth performance, improving immune functions, inhibition intestinal microbial pathogens and lowering cholesterol. Recently, it has been used as a feed additive in fish diets. Moreover, chitosan has gained an attention as an effective agent in aquaculture water treatment. A number of studies have been conducted on the effectiveness of the use of chitosan in aquaculture. Thus, the present review explored the current state of knowledge on the effects of dietary chitosan on farmed aquatic animals including antimicrobial, growth promoting, antioxidant activity and immunostimulation effects, with a particular emphasis on the optimal dose of chitosan. As well as, this review pointed out the possibility of using chitosan in aquaculture water treatment. According to the studies that were discussed in this review, chitosan is considered as a promising material with a massive potential of applications in aquaculture.

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“…Diets supplemented with chitosan for rainbow trout [ 83 ], olive flounder [ 84 ], koi [ 85 ], kelp grouper [ 86 ], turbot [ 87 ], gibel carp [ 88 ], mrigal carp [ 89 ], and Asian seabass [ 90 ] have proved that the chitosan could enhance growth, the innate immunity, disease, and stress resistance, improve haematological parameters and improve water quality. From fish immunological perspective, chitosan nanoparticles have been used for the delivery of vitamin C [ 91 ], RNA [ 92 ], or DNA [ 93 , 94 , 95 ] due to their positive charge and solubility in aqueous solution. In addition, chitosan protects encapsulated active compounds from the harsh conditions in the gastrointestinal tract and enhances their absorption [ 96 ].…”

Section: Nanodelivery Systemsmentioning

confidence: 99%

“…The body composition in terms of protein and lipid content was not affected by RNA-loaded chitosan nanoparticles (chitosan-NPs) while the growth, performance, immunity, and survival following a bacterial challenge ( Aeromonas hydrophila ) were significantly increased compared to only chitosan or bare RNA. Unaffected glucose and serum uric acid levels, and decreased transaminases and dehydrogenases, coupled with improved performance, indicated an enhanced energetic efficiency for anabolic processes and the safety of RNA-loaded chitosan-NPs as a nutraceutical [ 92 ] ( Table 2 ).…”

Section: Nanodelivery Systemsmentioning

confidence: 99%

Nanodelivery Systems as New Tools for Immunostimulant or Vaccine Administration: Targeting the Fish Immune System

Torrealba

Ruyra

et al. 2015

Biology

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Fish disease treatments have progressed significantly over the last few years and have moved from the massive use of antibiotics to the development of vaccines mainly based on inactivated bacteria. Today, the incorporation of immunostimulants and antigens into nanomaterials provide us with new tools to enhance the performance of immunostimulation. Nanoparticles are dispersions or solid particles designed with specific physical properties (size, surface charge, or loading capacity), which allow controlled delivery and therefore improved targeting and stimulation of the immune system. The use of these nanodelivery platforms in fish is in the initial steps of development. Here we review the advances in the application of nanoparticles to fish disease prevention including: the type of biomaterial, the type of immunostimulant or vaccine loaded into the nanoparticles, and how they target the fish immune system.

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RNA-Loaded Chitosan Nanoparticles for Enhanced Growth, Immunostimulation and Disease Resistance in Fish

Cited by 32 publications

References 53 publications

Molecular characterization, tissue distribution of Follicle-Stimulating Hormone (FSH) beta subunit and effect of kisspeptin-10 on reproductive hormonal profile ofCatla catla(Hamilton, 1822)

Molecular characterization, tissue distribution of Follicle-Stimulating Hormone (FSH) beta subunit and effect of kisspeptin-10 on reproductive hormonal profile ofCatla catla(Hamilton, 1822)

Effects of dietary chitosan supplementation on farmed fish; a review

Nanodelivery Systems as New Tools for Immunostimulant or Vaccine Administration: Targeting the Fish Immune System

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