RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus

Fāng, Yīng; Choi, Jae Young; Lee, Seok Hee; Kim, Jong Hoon; Park, Dong Hwan; Park, Min Gu; Woo, Ra Mi; Lee, Bo Ram; Kim, Woo Jin; Li, Shuo; Je, Yeon Ho

doi:10.1016/j.aspen.2017.08.011

Cited by 4 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA interference has now been widely used to study gene functions during virus transmission through their insect vectors [15,17,19]. To determine whether RNA interference can affect the expression of reference genes, we first silenced LsSYNJ1 expression in L. striatellus through microinjection.…”

Section: Resultsmentioning

confidence: 99%

“…RT-qPCR has been used to investigate gene expression in different tissues of L. striatellus nymphs, and in L. striatellus infected with different viruses [15,16]. It has also been used to validate the expression of specific genes identified through various transcriptomic and proteomic approaches, specific RNA transcript levels after the gene was silenced in L. striatellus , and virus accumulation in infected L. striatellus [6,7,15,17]. Because the reliability of RT-qPCR depends largely on the reference gene(s) used to normalize the data, it is crucial to know the stability of the reference gene(s) under specific experimental conditions prior to RT-qPCR [18].…”

Section: Introductionmentioning

confidence: 99%

“…The 18S ribosome RNA ( 18S rRNA ) gene was used as an reference gene for the quantification of RSV accumulation in L. striatellus at various developmental stages [22]. In a different report, ADP ribosylation factor ( LsARF ) was used to normalize the expression data of nuclear receptor E75 ( LsE75 ), which is known to suppress RSV transmission through L. striatellus [17]. The L. striatellus elongation factor 2 ( LsEF2 ) was used as a reference gene to determine the expression of host and viral genes in L. striatellus [23].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of Suitable RT-qPCR Reference Genes for Studies of Gene Functions in Laodelphax striatellus (Fallén)

Liu

Dong

et al. 2019

Genes

View full text Add to dashboard Cite

The reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been widely used to determine gene functions in Laodelphax striatellus (Fallén) (small brown planthopper). Selection of suitable reference gene(s) for normalizations of RT-qPCR data is critical for reliable results. To date, reports on identification of suitable L. striatellus reference genes are still very limited. L. striatellus is a destructive rice pest and it can transmit multiple viruses, including Rice black-streaked dwarf virus (RBSDV), Rice stripe virus (RSV), and Maize rough dwarf virus (MRDV), to many important cereal crops worldwide. In this study, we examined the stablity of seven selected candidate reference genes in L. striatellus at different developmental stages, in different tissues, in RBSDV- or RSV-infected L. striatellus or in RBSDV-infected and Lssynaptojanin 1 (LsSYNJ1)-silenced L. striatellus. The RT-qPCR data representing individual candidate genes were analyzed using five different methods: the delta Ct method, geNorm, NormFinder, BestKeeper, and the RefFinder algorithm, respectively. The most stable reference gene for the specific condition was selected according to a comprehensive analysis using the RefFinder method. Ribosomal protein L5 (LsRPL5) and LsRPL8 are the most stably expressed genes in L. striatellus at different developmental stages. Alpha-1-tubulin (Lsα-TUB) is the most stably expressed reference gene in different tissues of RBSDV viruliferous (RBSDV-V) or non-viruliferous (RBSDV-NV) L. striatellus. LsRPL8 is the most stably expressed reference gene in RBSDV-V or RSV viruliferous (RSV-V) L. striatellus, while beta-tubulin (Lsβ-TUB) is the most stably expressed reference gene in RBSDV-V and LsSYNJ1-silenced L. striatellus. The selected reference genes were further investigated during analyses of RBSDV P5-1 and P10 gene expression in different tissues from RBSDV-V or RBSDV-NV L. striatellus. The stably expressed reference genes identified in this study will benefit future gene function studies using L. striatellus.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Suitable RT-qPCR Reference Genes for Studies of Gene Functions in Laodelphax striatellus (Fallén)

Liu

Dong

et al. 2019

Genes

View full text Add to dashboard Cite

show abstract

“…In addition, the genome of L. striatellus was recently published, which will help to understand the genetic basis of virus–vector interactions . Several virus and insect proteins involved in RSV infection have been identified in some virus–vector combinations . However, the repertoire of genes involved in the RSV propagation process within the ovary during RSV infection has barely been explored.…”

Section: Introductionmentioning

confidence: 99%

The vitellogenin receptor has an essential role in vertical transmission of rice stripe virus during oogenesis in the small brown plant hopper

Lin

Ding

et al. 2018

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: The small brown plant hopper (SBPH), Laodelphax striatellus Fallén, is one of the most destructive pests on rice. This pest transmits rice stripe virus (RSV) both horizontally and vertically, leading to major yield and economic losses in rice production. However, the way that RSV particles enter oocytes of SBPH remains largely unknown. Thus, identification of key factors involved in the interaction between SBPH and RSV in the ovary is crucial. RESULTS: Transcriptome of non-viruliferous (NV) or high viruliferous (HV) SBPH ovaries at 24 and 48 h of emergence wassequenced. Differentially expressed genes analysis showed that vitellogenin receptor was significantly highly expressed in the ovary of the HV SBPH strains compared to NV strains. Quantitative real-time polymer chain reaction showed that the vitellogenin receptor in L. striatellus (LsVgR) was highly expressed in the ovaries of female adults and maintained a high level of expression at the early stage of ovary development. By using RNA interference, the expression of LsVgR in the ovaries of the HV strain was significantly decreased by 98.1%. RSV titer was reduced by 60.9% as quantified by viral RNA3 intergenic region and the transcripts of nucleocapsid protein gene (CP) reduced by 46.3%. The numbers of offspring hatched were significantly reduced in dsRNA-treated groups. The transcripts of CP were not affected by silencing LsVgR, whereas the abundance of RNA-dependent RNA polymerase increased by 15-fold in the member of surviving progenies. CONCLUSION: Our results suggest that vitellogenin receptor participates in regulating RSV replication during oogenesis.

show abstract

“…RNAi-mediated control of plant viruses in their vector insects could be achieved either directly by silencing of viral transcripts or indirectly by silencing host genes essential for viral replication (Kanakala and Ghanim, 2016). Replication of RSV in L. striatellus has been efficiently suppressed not only by application of exogenous RSV-specific dsRNAs (An et al, 2017) but also by transcriptional silencing of L. striatellus genes, specifically, CRP1 and LsE75 (Fang et al, 2017;Liu et al, 2015). In a previous comparative transcriptomic analysis, we identified L. striatellus genes that are strongly upregulated in RSV-viruliferous L. striatellus (Lee et al, 2013).…”

mentioning

confidence: 99%

Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs

et al. 2020

Self Cite

View full text Add to dashboard Cite

RNA interference (RNAi) has attracted attention as a promising approach to control plant viruses in their insect vectors. In the present study, to suppress replication of the rice stripe virus (RSV) in its vector, Laodelphax striatellus, using RNAi, dsRNAs against L. striatellus genes that are strongly upregulated upon RSV infection were delivered through a rice leaf-mediated method. RNAi-based silencing of peroxiredoxin, cathepsin B, and cytochrome P450 resulted in significant down regulation of the NS3 gene of RSV, achieving a transcriptional reduction greater than 73.6% at a concentration of 100 ng/µl and, possibly compromising viral replication. L. striatellus genes might play crucial roles in the transmission of RSV; transcriptional silencing of these genes could suppress viral replication in L. striatellus. These results suggest effective RNAi-based approaches for controlling RSV and provide insight into RSV-L. striatellus interactions.

show abstract

RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus

Cited by 4 publications

References 38 publications

Determination of Suitable RT-qPCR Reference Genes for Studies of Gene Functions in Laodelphax striatellus (Fallén)

Determination of Suitable RT-qPCR Reference Genes for Studies of Gene Functions in Laodelphax striatellus (Fallén)

The vitellogenin receptor has an essential role in vertical transmission of rice stripe virus during oogenesis in the small brown plant hopper

Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs

Contact Info

Product

Resources

About