RNA Helicases: Versatile ATP-Driven Nanomotors

Jankowsky, Eckhard; Fairman, Margaret E.; Yang, Quansheng

doi:10.1166/jnn.2005.508

Cited by 20 publications

(12 citation statements)

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“…µ was used to denote the expectation of yields when there was no mutant, hence µ A ( X i , Z,K ) could be considered the theoretical yield of phi29 virion at the condition X i . log 10 ( Y i )~ N (log 10 (µ A ( X i , Z,K )), 2 was assumed. Thus, measurement errors for Y i increased exponentially as the percentage of functional motors, A ( X i , Z,K ), increased.…”

Section: Resultsmentioning

confidence: 99%

Binomial distribution for quantification of protein subunits in biological Nanoassemblies and functional nanomachines

Fang¹,

Zhang²,

Huang³

et al. 2014

Nanomedicine: Nanotechnology, Biology and Medicine

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Living systems produce ordered structures and nanomachines that inspire the development of biomimetic nanodevices such as chips, MEMS, actuators, sensors, sorters, and apparatuses for single-pore DNA sequencing, disease diagnosis, drug or therapeutic RNA delivery. Determination of the copy numbers of subunits that build these machines is challenging due to small size. Here we report a simple mathematical method to determine the stoichiometry, using phi29 DNA-packaging nanomotor as a model to elucidate the application of a formula ∑M=0Ztrue(ZMtrue)pZ−MqM, where p and q are the percentage of wild-type and inactive mutant in the empirical assay; M is the copy numbers of mutant and Z is the stoichiometry in question. Variable ratios of mutants and wild-type were mixed to inhibit motor function. Empirical data were plotted over the theoretical curves to determine the stoichiometry and the value of K, which is the number of mutant needed in each machine to block the function, all based on the condition that wild-type and mutant are equal in binding affinity. Both Z and K from 1–12 were investigated. The data precisely confirmed that phi29 motor contains six copies (Z) of the motor ATPase gp16, and K = 1.

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Section: Resultsmentioning

confidence: 99%

Binomial distribution for quantification of protein subunits in biological Nanoassemblies and functional nanomachines

Fang¹,

Zhang²,

Huang³

et al. 2014

Nanomedicine: Nanotechnology, Biology and Medicine

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show abstract

“…These include RNA helicases, actin‐myosin, dyneins and kinesins, among others (Hess et al. 2004; Jankowsky et al. 2005).…”

Section: Applications In Nanobiotechnologymentioning

confidence: 99%

What Sperm Can Teach us About Energy Production

Mukai

Travis

2012

Reprod Domestic Animals

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Mammalian sperm have evolved under strict selection pressures that have resulted in a highly polarized and efficient design. A critical component of that design is the compartmentalization of specific metabolic pathways to specific regions of the cell. Although the restricted localization of mitochondria to the midpiece is the best known example of this design, the organization of the enzymes of glycolysis along the fibrous sheath is the primary focus of this review. Evolution of variants of these metabolic enzymes has allowed them to function when tethered, enabling localized energy production that is essential for sperm motility. We close by exploring how this design might be mimicked to provide an energy-producing platform technology for applications in nanobiotechnology.

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“…Obvious candidates for factors involved in remodeling RNPs during localization are RNA helicases. Many aspects of RNA metabolism involve helicases, which can act in an ATP-dependent manner to either unwind duplex RNA or alter protein-RNA interactions (reviewed in Jankowsky et al, 2005). Recently, Fairman et al (2004) demonstrated that RNA helicases can remove proteins from an RNA duplex without unwinding of the RNA suggesting that the ''RNPases'' can remodel a variety of RNP interactions.…”

Section: Remodeling By Rearrangement: Helicases At Work?mentioning

confidence: 99%