RNA CHANGES IN ENVIRONMENTALLY INDUCED FLAX (LINUM USITATISSIMUM) GENOTROPHS

Abstract

Purified samples of WNA were obtained from large (L) and stnall (s) flax genotrophs envirc~nrneiatally induced within the flax genotype Stormont Cirrus. The RNA san~ples were electrophc~resed to separate 25S, 23S, 18S, 16S, 5 s and 4S RNA's, using 2.4% and 5% acrylamide gels. Nucleotide analysis of the total RNA of each of the samples was also carried out. Differences between L and S In the relative amounts of the variou:, RNA species were detectable. Analogous differences between I, and S for the relative amounts of the four bases in the total WN.4 were also detectable.Des dchantillons purifiks (B'ARN ant i t i tirds de grands ( L ) et des petits (S) ginotropes du Bi n crkk:, dans leur ~llilieu au sein d'un peuplernent de gknotype Stor~nont Cirrus. Les echrantillon:, ont et6 soumis a l'@lectrophorken gel acrylamide a 2,4% et Scii de f a~o n i permettre la cdparation des fractions 2 S S , 2 3 s . LMS, 16s. 5s et 4 s . L'anaiqse des nucliotides pour !'ensemble de I'ARN contenu dans chaque Cchantillon a kgalernent Ot C effectui'e, et on a constate I'existence d'une nette difference au iliveau de la quantitd dec divers types d9ARN entre Bes gknoeropes I, et S. Une diffOrence sembiiabie entre les deux ginotropes existait Cgalerrlent pour la proportion relative des quatre base:, nuclkiqucs.LTraduit par Ic journal]