Preliminary characterization of peroxidase isozymes isolated from two flax genotrophs

Fieldes, Mary Ann; Deal, Cheri; Tyson, H.

doi:10.1139/b77-172

Cited by 23 publications

(5 citation statements)

References 16 publications

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“…However, the exact interrelationships between variation in phenotypic characteristics on the one hand, and variation in rRNA, tRNA and nuclear DNA on the other, remain obscure. Moreover, larger amounts of one particular enzyme, peroxidase, are produced in S compared to L (Fieldes et al, 1977), so that a correlation of this type in the genotrophs may well involve a complex of interactions between, for example, the plant hormone systems and their inhibitors or promoters.…”

Section: Resultsmentioning

confidence: 99%

RNA CHANGES IN ENVIRONMENTALLY INDUCED FLAX (LINUM USITATISSIMUM) GENOTROPHS

El-Gewely¹,

Tyson²

1978

Can. J. Genet. Cytol.

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Purified samples of WNA were obtained from large (L) and stnall (s) flax genotrophs envirc~nrneiatally induced within the flax genotype Stormont Cirrus. The RNA san~ples were electrophc~resed to separate 25S, 23S, 18S, 16S, 5 s and 4S RNA's, using 2.4% and 5% acrylamide gels. Nucleotide analysis of the total RNA of each of the samples was also carried out. Differences between L and S In the relative amounts of the variou:, RNA species were detectable. Analogous differences between I, and S for the relative amounts of the four bases in the total WN.4 were also detectable.Des dchantillons purifiks (B'ARN ant i t i tirds de grands ( L ) et des petits (S) ginotropes du Bi n crkk:, dans leur ~llilieu au sein d'un peuplernent de gknotype Stor~nont Cirrus. Les echrantillon:, ont et6 soumis a l'@lectrophorken gel acrylamide a 2,4% et Scii de f a~o n i permettre la cdparation des fractions 2 S S , 2 3 s . LMS, 16s. 5s et 4 s . L'anaiqse des nucliotides pour !'ensemble de I'ARN contenu dans chaque Cchantillon a kgalernent Ot C effectui'e, et on a constate I'existence d'une nette difference au iliveau de la quantitd dec divers types d9ARN entre Bes gknoeropes I, et S. Une diffOrence sembiiabie entre les deux ginotropes existait Cgalerrlent pour la proportion relative des quatre base:, nuclkiqucs.LTraduit par Ic journal]

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Section: Resultsmentioning

confidence: 99%

RNA CHANGES IN ENVIRONMENTALLY INDUCED FLAX (LINUM USITATISSIMUM) GENOTROPHS

El-Gewely¹,

Tyson²

1978

Can. J. Genet. Cytol.

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“…In contrast, A.Prx 1a had the same charge as A.Prx 1 and its molecular weight increase (about 8 000) suggested a difference in glycosylation. If so, estimates of carbohydrte contents for the anionic stem isozymes [6] indicate that the carbohydrate moiety of A.Prx 1a would be twice the size of that for A.Prx 1. Apart from these two situations, none of the other differences among the isozymes, in charge, M r , or both, could be explained in terms of altered post-translational modification.…”

Section: Discussionmentioning

confidence: 99%

“…There are also two root-specific isozymes; however, there are at least four other isozymes that are not tissuespecific and a number of additional isozymes that are induced when the plants are stressed [5]. Furthermore, five of the isozymes found in nonstressed plants are known to be glycosylated [6,7], and it is reasonable to expect that most, if not all, of the other isozymes will also be glycoproteins. N-linked glycosylation is a common fea- …”

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Charge and molecular weight differences for fourteen guaiacol peroxidase isozymes in flax indicate that most are encoded by different structural genes

Gerhardt

Fieldes

1999

Electrophoresis

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Ferguson plots, linear regressions of log(relative mobility) against polyacrylamide gel concentration, provide an electrophoretic method of simultaneously obtaining estimates of net charge and molecular weight for nondenatured proteins, and are a powerful method of accurately comparing these characteristics among isozymes within an enzyme system. Here, Ferguson plots compare the characteristics of the nine isozymes (three cationic and six anionic) usually seen in soluble extracts of flax (Linum usitatissimum) seedling tissues, and of five anionic isozymes induced by heavy metal stress. Despite some differences in apparent molecular weight, all but one of the isozymes separated from each other on the basis of net charge and, except for a second isozyme with a comparatively high molecular weight, the net charges of the isozymes ranked in order of their relative mobilities on nondenaturing gels. Differences among the isozymes make it unlikely that any are related, which indicates that they are encoded by different structural genes.

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“…Isozymes of the plant peroxidases extend their functional range. Structural features surrounding haem group may affect peroxidase compared with IAA oxidase activity (Job and Ricard 1975;Fieldes et al 1977;Gaspar et al 1980). One or more (-Asp-X-(Ser1Thr)-) sites are found, allowing oligosaccharide chains to be attached to the polypeptide and subsequently modified, e.g .…”

Section: Introductionmentioning

confidence: 99%

Relationships, derived from optimum alignments, among amino acid sequences of plant peroxidases

Tyson¹

1992

Can. J. Bot.

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TYSON, H. 1992. Relationships, derived from optimum alignments, among amino acid sequences of plant peroxidases. Can. J. Bot. 70: 543-556. The amino acid and (or) DNA sequences of 13 plant peroxidases (EC 1.1 1.1.7), which include isozymes within species, are currently available in data bases; all have similar lengths of approximately 300 amino acids. Sequence relationships among these 13, plus 2 microbial peroxidases of similar length, were examined. The 15 sequences were compared in all 105 pairwise combinations using optimum alignment procedures. Gap penalties were deterrnined from analysis of penalty change effects. Distances between sequences generated by optimum alignments were analysed by clustering techniques to generate dcndrograms. Specific distances, which provided pairwise distance measurements independent of the average distance for a sequence, were used to evaluate sequence similarities; closely related sequences produce closely correlated specific distances. Among the seven plant species, five subgroups were established: (1) horseradish isopcroxidases, (2) turnip and wheat, (3) cucumber and tobacco, (4) potato and tomato, and (5) in which cytochrome c peroxidase showed some similarity to ligninase, but both were only distantly related to plant peroxidases. Horseradish isoperoxidases werc related to sequences in subgroups 2 , 3 , and 4 but resembled subgroups 2 and 3 more closely than 4. Subgroup 2 was more related to 3 than any other.

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Preliminary characterization of peroxidase isozymes isolated from two flax genotrophs

Cited by 23 publications

References 16 publications

RNA CHANGES IN ENVIRONMENTALLY INDUCED FLAX (LINUM USITATISSIMUM) GENOTROPHS

RNA CHANGES IN ENVIRONMENTALLY INDUCED FLAX (LINUM USITATISSIMUM) GENOTROPHS

Charge and molecular weight differences for fourteen guaiacol peroxidase isozymes in flax indicate that most are encoded by different structural genes

Relationships, derived from optimum alignments, among amino acid sequences of plant peroxidases

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