RNA aptamers to the peptidyl transferase inhibitor chloramphenicol

Burke, Donald H.; Hoffman, David C.; Brown, Analisa; Hansen, Mark; Pardi, Arthur; Gold, Larry

doi:10.1016/s1074-5521(97)90116-2

Cited by 79 publications

(56 citation statements)

References 32 publications

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“…RNA 2100 -- (Burke et al, 1997) Tetracyclines RNA 1000 -- (Berens et al, 2001) DNA 64 Electrochemical 10 nM Tetracycline DNAElectrochemical 1 ng/mL (Zhang et al, 2010) -- (Niazi, Lee, Kim et al, 2008) Electrochemical -(Y. S. Oxytetracycline DNA 10…”

Section: Chloramphenicolmentioning

confidence: 99%

Advances in Aptamer-Based Biosensors for Food Safety

McKeague¹,

Giamberardino²,

DeRosa³

2011

Environmental Biosensors

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Section: Chloramphenicolmentioning

confidence: 99%

Advances in Aptamer-Based Biosensors for Food Safety

McKeague¹,

Giamberardino²,

DeRosa³

2011

Environmental Biosensors

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“…We mutated both the enriched pool and the best binding monoclone K11 by EP-PCR (Cadwell and Joyce 1992) and reselected under more stringent conditions. Off-rate selection has proven an efficient means to evolve tighter binders (Burke et al 1997;Zahnd et al 2004). In our case, the K D value of the original best binder K11 (4.1 mM) was reduced >10-fold.…”

Section: Discussionmentioning

confidence: 99%

RNA aptamers selectively modulate protein recruitment to the cytoplasmic domain of β-secretase BACE1 in vitro

Rentmeister¹,

Bill²,

Wahle³

et al. 2006

RNA

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The b-amyloid peptide (Ab) is a major component of the Alzheimer's disease (AD)-associated senile plaques and is generated by sequential cleavage of the b-amyloid precursor protein (APP) by b-secretase and g-secretase. Since BACE1 initiates Ab generation it represents a valuable target to interfere with Ab production and treatment of AD. While the enzymatic activity of BACE1 resides in the extracellular domain, the protein also contains a short cytoplasmic tail (B1-CT). This domain serves as a binding site for at least two proteins, the copper chaperone for superoxide dismutase-1 (CCS), and the Golgi-localized, g-earcontaining, ADP ribosylation factor-binding (GGA1) protein, and contains a single phosphorylation site. However, the precise role of the B1-CT for the overall biological function of this protein is largely unknown. Functional studies focusing on the activity of this domain would strongly benefit from the availability of domain-specific inhibitors. Here we describe the isolation and characterization of RNA aptamers that selectively target the B1-CT. We show that these RNAs bind to authentic BACE1 and provide evidence that the binding site is restricted to the membrane-proximal half of the C terminus. Aptamer-binding specifically interferes with the recruitment of CCS, but still permits GGA1 association and casein kinase-dependent phosphorylation, consistent with selective binding site targeting within this short peptide. Because phosphorylation and GGA1 binding to B1-CT regulate BACE1 transport, these RNA inhibitors could be applied to investigate B1-CT activity without affecting the subcellular localization of BACE1.

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“…Tried to image tenascin-C and nucleolin simultaneously using two types of aptamer-conjugated QDs. they showed that this imaging was reliable and cell line-specific; however, current requirements for cancer imaging technologies include the need for simple preparation methods and the ability to detect multiple cancer biomarkers and evaluate their intracellular localizations [65,66].…”

Section: Cancer Cell Imaging By Aptamer-conjugated Qdsmentioning

confidence: 99%

A Review on Aptamer-Conjugated Quantum Dot Nanosystems for Cancer Imaging and Theranostic

Johari-Ahar¹

2017

JNMR

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Over the last 10 years, fluorescent semiconductor QD (quantum dot)-aptamer conjugates have emerged as an efficient platform for cancer imaging and therapy in animal models and in vitro. In addition, these conjugates show potential in a wide range of applications in environmental monitoring, disease diagnosis, and bio-sensing. The present review represents the recent developments in QDaptamer bio-conjugates for applications in cancer studies. It starts with a brief introduction to Semiconductor Quantum dots (QDs), bio-conjugation of QDs and aptamer molecules, and advantages-disadvantages of using these novel tools for biochemical applications.

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RNA aptamers to the peptidyl transferase inhibitor chloramphenicol

Cited by 79 publications

References 32 publications

Advances in Aptamer-Based Biosensors for Food Safety

Advances in Aptamer-Based Biosensors for Food Safety

RNA aptamers selectively modulate protein recruitment to the cytoplasmic domain of β-secretase BACE1 in vitro

A Review on Aptamer-Conjugated Quantum Dot Nanosystems for Cancer Imaging and Theranostic

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