Abstract:RmaI, an isoschizomer of MaeI (1) has been isolated from Rhodothermus marinus (2). RmaI recognizes the palindromic sequence 5'-CTAG-3'. Like its isoschizomer RmaI cleaves the sequence, C/TAG, generating 5'-protruding TA-dinucleotides.The recognition sequence of RmaI was determined using double digestions on pBR322and phiXI74 DNAs (figure 1, lanes 2-6 and 8-12). The cleavage patterns obtained were compared with computer-derived mapping data (3). The data predicts the sequence 5'-CTAG-3'. The sequence was also t… Show more
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