The Genera Rhodothermus, Thermonema, Hymenobacter and Salinibacter

Phylogeny and molecular signatures (conserved proteins and indels) that are specific for the Bacteroidetes and Chlorobi species

“…In contrast to these trees, when the same dataset was analyzed by means of the character compatibility or clique approach, S. ruber formed the deepest branch of the Bacteroidetes species and its specific association with this group was supported by 21 uniquely shared characters, indicating strongly that this affiliation was reliable [57]. These results provide evidence that the character compatibility approach, which removes all fast-evolving as well as homoplasic sites from a given dataset, provides a powerful means for obtaining correct topology in cases, such as that for S. ruber , whose phyletic affinity has proven difficult to establish by traditional phylogenetic methods [13,52,56,57]. …”

“…The bacteria from the Bacteroidetes phylum (previously known as the Cytophaga-Flavobacteria-Bacteroides (CFB) group) exhibit a potpourri of phenotypes including gliding behavior and their ability to digest and grow on a variety of complex substrates such as cellulose, chitin and agar [4-8]. They inhabit diverse habitats including the oral cavity of humans, the gastrointestinal tracts of mammals, saturated thalassic brines, soil and fresh water [9-13]. The Bacteroides species such as B. thetaiotaomicron and B. fragilis are among the dominant microbes in the large intestine of human and other animals [14,15].…”

Selective enrichment, isolation and molecular detection of Salinibacter and related extremely halophilic Bacteria from hypersaline environments

“…Salinibacter is inhibited by high levels of nutrients (Anton et al, 2002;Oren, 2004;Oren et al, 2004), and we therefore used low nutrient media throughout this study. Two antibiotics proved suitable as selective agents: anisomycin and bacitracin.…”

“…It is an obligate halophile that requires at least 150 g l -1 salt, and grows optimally between 200 and 300 g l -1 . Physiologically, the organism is surprisingly similar to the halophilic red Archaea: the cells maintain high intracellular K + concentrations, possess salt-dependent enzymes, and have proteins with a high content of acidic amino acids (Antó n et al, 2002;Oren & Mana, 2003;Oren et al, 2004;Oren, 2004). S. ruber is colored red due to the presence of a novel C-40 carotenoid acyl glucoside named salinixanthin (Lutnaes et al, 2002).…”

Salinibacter: an extremely halophilic bacterium with archaeal properties

“…Two approaches independently led to the recovery of similar isolates: (1) hybridization of red colonies with 16S rRNA gene probes directed against the EHB phylotype; and (2) polar lipid analysis by thin‐layer chromatography, showing that some red‐orange colonies contained not Archaea but Bacteria. The isolates were subjected to a taxonomic description (Antón et al ., , ; Oren, ). Procedures were designed to selectively enrich and isolate Salinibacter and relatives from hypersaline brines, based on the use of antibiotics (anisomycin, bacitracin) that inhibit Halobacteriaceae but do not affect Salinibacter (Elevi Bardavid et al ., ).…”