Risperidone Release from Solid Lipid Nanoparticles (SLN): Validated HPLC Method and Modelling Kinetic Profile

Silva, Ana Catarina; Lopes, Carla Martins; Fonseca, Juliano; Soares, Maria Elisa; Santos, Delfim; Souto, Eliana B.; Ferreira, Domingos

doi:10.2174/157341212803341663

Cited by 28 publications

(20 citation statements)

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“…Several other mathematical dissolution models are built into the DDSolver program to estimate the drug release mechanisms, such as the Hixson-Crowell (H-C) model [34, 35], Baker-Lonsdale (B-L) model [36, 37], and Hopfenberg model [38]. Because the degradation of gelatin nanoparticles is characterized by a homogeneous erosion as mentioned above, the H-C model, which describes the release profile of drug particles with a diminishing surface area during dissolution [39], and the Hopfenberg model, which describes the heterogeneous erosion [40], cannot be applied.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the DDSolver Software Applications

Zuo

Gao

Bou‐Chacra

et al. 2014

BioMed Research International

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When a new oral dosage form is developed, its dissolution behavior must be quantitatively analyzed. Dissolution analysis involves a comparison of the dissolution profiles and the application of mathematical models to describe the drug release pattern. This report aims to assess the application of the DDSolver, an Excel add-in software package, which is designed to analyze data obtained from dissolution experiments. The data used in this report were chosen from two dissolution studies. The results of the DDSolver analysis were compared with those obtained using an Excel worksheet. The comparisons among three different products obtained similarity factors (f 2) of 23.21, 46.66, and 17.91 using both DDSolver and the Excel worksheet. The results differed when DDSolver and Excel were used to calculate the release exponent “n” in the Korsmeyer-Peppas model. Performing routine quantitative analysis proved to be much easier using the DDSolver program than an Excel spreadsheet. The use of the DDSolver program reduced the calculation time and has the potential to omit calculation errors, thus making this software package a convenient tool for dissolution comparison.

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Section: Resultsmentioning

confidence: 99%

Evaluation of the DDSolver Software Applications

Zuo

Gao

Bou‐Chacra

et al. 2014

BioMed Research International

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“…These results demonstrate the specificity of the proposed HPLC-PDA to identify and to quantify the trans isomeric form (tRES) released from SLN. Method specificity was analyzed with respect to the risk of interference between the lipids and surfactants composing SLN and their effect in the tRES retention time [46]. The three distinct samples were filtered before analysis by the proposed HPLC method.…”

Section: Resultsmentioning

confidence: 99%

“…Robustness can be measured by assessing the effect of small fluctuations in the chromatographic experimental conditions on the retention time and obtained RSD value [46]. Robustness was evaluated by analyzing the 50.0 µg/mL tRES standard solution.…”

Section: Resultsmentioning

confidence: 99%

“…Stearic acid has been selected as the solid lipid from the preliminary screening of the solubility of tRES in a set of solid lipids (data not shown), while the surfactant combination has been based on reports from scientific literature [46]. SLN with a mean hydrodynamic diameter of 237.03 ± 1.80 nm (F1.tRES) and 201 ± 2.04 nm (F2.tRES), and a polydispersity index of 0.206 ± 0.012 nm (F1.tRES) and 0.153 ± 0.061 nm (F2.tRES) were obtained by ultrasound technique, and the validated method was applied for the quantification of tRES released from SLN in Franz diffusion cells.…”

Section: Resultsmentioning

confidence: 99%

“…Specificity translates the capacity of the method to discriminate between tRES and the other closely related structures present in the samples, such as lipids and surfactants [46]. Specificity was assessed by analyzing the filtrate of drug-free SLN (10.000 NMWL membranes, Millipore, Billerica, MA, USA) and further comparing the resulting chromatogram with that obtained for 50.0 µg/mL tRES standard sample.…”

Section: Specificitymentioning

confidence: 99%

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Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array

Rigon

Fachinetti

Severino³

et al. 2019

Applied Sciences

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A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0–3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6–5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9–10 min. The flow rate was set at 1.0 mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1–100 µg/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 ± 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.

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